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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz


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Volume 6, Issue 3

Issues

Volume 10 (2015)

Combined action of X-rays and nonylphenol on mouse sperm

Małgorzata Dobrzyńska
  • Department of Radiation Protection and Radiobiology, National Institute of Public Health — National Institute of Hygiene, 00-791, Warsaw, Poland
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Published Online: 2011-04-27 | DOI: https://doi.org/10.2478/s11535-011-0021-0

Abstract

The aim of this study was to assess the effects of 2-weeks’ X-ray and/or nonylphenol (NP) exposure on male mice’s sperm count and quality. Pzh:SFIS mice were exposed to X-rays (0.05 Gy, 0.10 Gy, 0.20 Gy) or to nonylphenol (25 mg/kg bw, 50 mg/kg bw, 100 mg/kg bw) or to both agents (0.05 Gy + 25 mg/kg bw NP, 0.10 Gy + 50 mg/kg bw NP). At 24 h and 5 weeks after the end of exposure the sperm count, morphology and frequency of DNA damage in the male germ cells were estimated. Each agent alone diminished sperm count and morphology. The dose of 0.05 Gy of X-rays decreased the frequency of DNA damage. Combined exposure to lower doses of both agents significantly improved sperm morphology and decreased the level of DNA damage compared to one agent alone. Combined exposure to higher doses reduced the frequency of DNA damage compared to the effect of the appropriate dose of NP. Results of combined exposure to low doses of both agents suggest that 0.05 Gy of X-rays stimulate the DNA damagecontrol system and in consequence repair of DNA caused by X-rays and NP. It may be correlated with increased antioxidant capacity.

Keywords: Sperm count and morphology; Dna damage; X-Rays; Nonylphenol; Combined exposure

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About the article

Published Online: 2011-04-27

Published in Print: 2011-06-01


Citation Information: Open Life Sciences, Volume 6, Issue 3, Pages 320–329, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-011-0021-0.

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