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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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Volume 7, Issue 1


Volume 10 (2015)

Effectiveness of the entomopathogenic nematode Steinernema feltiae in agar gel formulations against larvae of the Colarado potato beetle, Leptinotarsa decemlineata (Say.) (Coleoptera: Chrysomelidae)

Hany Hussein
  • Biology Centre, Academy of Sciences of the Czech Republic, Institute of Entomology, 370 05, České Budějovice, Czech Republic
  • Pests & Plant Protection Department, National Research Centre, 12311, Cairo, Egypt
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/ Manal Adel / Ivan Gelbič
  • Biology Centre, Academy of Sciences of the Czech Republic, Institute of Entomology, 370 05, České Budějovice, Czech Republic
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Published Online: 2011-12-25 | DOI: https://doi.org/10.2478/s11535-011-0090-0


The entomopathogenic nematode Steinernema feltiae strain Ustinov Russia was used on potato foliage to control larvae of the Colorado potato beetle, Leptinotarsa decemlineata (Say.) (Coleoptera: Chrysomelidae). Nematodes were applied in formulations of agar at 4%, 2%, 1% and 0.5% concentrations and compared to a control application of nematodes in water for nematode survival. Agar formulation significantly improved efficacy by increasing nematode survival through reduction in desiccation when compared to water-based formulation. More than 70% of infective juvenile nematodes (IJs) died after being incubated in the highest concentration of agar for 12 h, while only 8% mortality was recorded at the 1% concentration. Suspension of nematodes in 1% agar gel was shown to be efficacious in both laboratory and greenhouse tests for extension of the nematodes’ survival. Agar formulation droplets dried slower than control droplets by 127.8 min. Leptinotarsa decemlineata mortality significantly increased when insects were exposed to infective juvenile nematodes for four hours after application. In conclusion, the agar formulation enhanced nematode survival by providing a suitable environment thereby delaying dryingand increasing the possibility for nematodes to invade their host on the foliage.

Keywords: Anti-desiccant; Foliar application; Drought; Foliar treatments; Extended survival time

  • [1] Metcalf R.L., Metcalf R.A., Destructive and Useful Insects, 5th ed. McGraw-Hill Book Co., New York, 1993, pp. 1443–1445 Google Scholar

  • [2] Jörg E., Beck W., Yield losses caused by the Colorado Potato Beetle and its control, Kartoffelbau, 2000, 51, 202–204 (in German) Google Scholar

  • [3] Schroer S., Ehlers R.U., Foliar application of the entomopathogenic nematode, Steinernema carpocapsae for biological control of diamond black moth larvae (Plutella xylostella), Biological Control, 2005, 33, 81–86 http://dx.doi.org/10.1016/j.biocontrol.2004.12.009CrossrefGoogle Scholar

  • [4] Schroer S., Ziermann D., Ehlers R.U., Mode of action of a surfactant-polymer formulation to support performance of the entomopathogenic nematode Steinernema carpocapsae for control of diamondback moth larvae (Plutella xylostella), Biocontrol Sci. Techn., 2005, 15, 601–613 http://dx.doi.org/10.1080/09583150500088694CrossrefGoogle Scholar

  • [5] Schroer S., Sulistyanto D., Ehlers R.U., Control of Plutella xylostella using polymerformulated Steinernema carpocapsae and Bacillus thuringiensis in cabbage fields, J. Appl. Nemato., 2005, 129, 198–204 Google Scholar

  • [6] Piggott S.J., Wright D.J. Matthews G.A., Polymeric formulation for the application of entomopathogenic nematodes against foliar pest, l Proceding of the Britsh Crop Production Council BCPC Conference on Pests and Diseases, Brighton, UK, 3, 2000, pp. 1063–1068 Google Scholar

  • [7] Navon A., Nagalakshmi V.K., Shlomit L., Salame L. Glazer I., Effectiveness of entomopathogenic nematodes in an alginate gel formulation against lepidopterous pests, Biocontrol Sci. Techn., 2002, 12, 737–746 http://dx.doi.org/10.1080/0958315021000039914CrossrefGoogle Scholar

  • [8] Lewis E.E., Gaugler R., Harrison R., Effectiveness of the entomopathogenic nematode Steinernema feltiae in agar gel formulations against Colorado potato beetle, Leptinotarsa decemlineata (Say.) (Coleoptera: Chrysomelidae), larvae, Entomopathogenic host finding: response to host cues by cruise and ambush foragers, Parasitology, 1992, 105, 309–315 http://dx.doi.org/10.1017/S0031182000074230CrossrefGoogle Scholar

  • [9] Grewal P.S., Gauler R., Lewis E.E., Host recognition behavior by entomopathogenic nematodes during contact with insect gut contents, J. Parasitol., 1993, 79, 495–503 http://dx.doi.org/10.2307/3283373CrossrefGoogle Scholar

  • [10] McGuire M.R., Shasha B.S., Adherent starch granules for encapsulation of insect control agents, J. Econ. Entomol., 1992, 85, 1425–1433 Google Scholar

  • [11] Woodring J.L., Kaya H.K., Steinernematid and heterorhabditid nematodes: a handbook of techniques, Arkansas Agricultural Experiment Station Southern Cooperative Series Bulletin 331, 1988, pp. 30 Google Scholar

  • [12] Mauléon H., Briand S., Laumond C., Bonifassi E., Use of digestive enzymes for the study of the parasitism of Steinernema and Heterorhabditis in insect larvae, Fundam. Appl. Nematol., 1993, 16, 185–191 (in French) Google Scholar

  • [13] Shapiro-Ilan D.I., Gouge G.H., Piggott S.J., Patterson Fife J., Application technology and environmental considerations for use of entomopathogenic nematodes in biological control, Biological Control, 2006, 38, 124–133 http://dx.doi.org/10.1016/j.biocontrol.2005.09.005CrossrefGoogle Scholar

  • [14] Lacey L.A., Shapiro-Ilan D.I., Microbial control of insect pests in temperate orchard systems: potential for incorporation into IPM, Annu Rev Entomol, 2008, 53, 121–144 http://dx.doi.org/10.1146/annurev.ento.53.103106.093419CrossrefGoogle Scholar

  • [15] Glazer I., Klein M., Navon A., Nakache Y., Comparison of efficacy of entomopathogenic nematodes combined with anti-desiccants applied by canopy sprays against three cotton pests (Lepidoptera: Noctuidae), J Econ Entomol, 1992, 85, 1636–1641 Google Scholar

  • [16] Baur M.E., Kaya H.K., Gaugler R., Tabashnik B.E., Effects of adjuvants on entomopathogenic nematode persistence and efficacy against Plutella xylostella, Biocontrol Sci Tech, 1997, 7, 513–525 http://dx.doi.org/10.1080/09583159730587CrossrefGoogle Scholar

  • [17] Head J., Lawrence A.J., Walters K.F.A., Efficacy of the entomopathogenic nematode, Steinernema feltiae, against Bemisia tabaci in relation to plant species, J Appl Entomol, 2004, 128, 543–547 http://dx.doi.org/10.1111/j.1439-0418.2004.00882.xCrossrefGoogle Scholar

  • [18] Shapiro-Ilan D.I., Cottrell T.E., Mizell III R.F., Horton D.L., Behle R.W., Dunlap C.A., Efficacy of Steinernema carpocapsae for control of the lesser peachtree borer, Synanthedon pictipes: Improved aboveground suppression with a novel gel application, Biological Control, 2010, 54, 23–28 http://dx.doi.org/10.1016/j.biocontrol.2009.11.009CrossrefGoogle Scholar

About the article

Published Online: 2011-12-25

Published in Print: 2012-02-01

Citation Information: Open Life Sciences, Volume 7, Issue 1, Pages 77–82, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-011-0090-0.

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© 2011 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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