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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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Volume 7, Issue 4


Volume 10 (2015)

In vitro propagation of Gentiana dinarica Beck.

Branka Vinterhalter
  • Plant Physiology Department, Institute for Biological Research “Siniša Stanković”, University of Belgrade, 11 000, Belgrade, Serbia
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/ Dijana Milošević
  • Plant Physiology Department, Institute for Biological Research “Siniša Stanković”, University of Belgrade, 11 000, Belgrade, Serbia
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/ Teodora Janković / Jelena Milojević
  • Plant Physiology Department, Institute for Biological Research “Siniša Stanković”, University of Belgrade, 11 000, Belgrade, Serbia
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/ Dragan Vinterhalter
  • Plant Physiology Department, Institute for Biological Research “Siniša Stanković”, University of Belgrade, 11 000, Belgrade, Serbia
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Published Online: 2012-06-03 | DOI: https://doi.org/10.2478/s11535-012-0059-7


Gentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l−1 BA and 0.1 mg l−1 NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5–1.0 mg l−1 IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.

Keywords: Gentiana dinarica; In vitro propagation; Micropropagation; Rooting; Endangered species

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About the article

Published Online: 2012-06-03

Published in Print: 2012-08-01

Citation Information: Open Life Sciences, Volume 7, Issue 4, Pages 690–697, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-012-0059-7.

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© 2012 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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