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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz


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2391-5412
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Volume 7, Issue 4

Issues

Volume 10 (2015)

Plant tissue cultures from four Tuscan globe artichoke cultivars

Laura Bedini / Mariella Lucchesini / Francesco Bertozzi / Alberto Graifenberg
Published Online: 2012-06-03 | DOI: https://doi.org/10.2478/s11535-012-0064-x

Abstract

The aim of the study was to examine the possibility of propagating in vitro four of the most common cultivars in Tuscany (central Italy): Terom, Violetto di Toscana, Chiusure and Empolese. The first three belong to the “Violetti” group, while cv Empolese belongs to the “Romaneschi” group. Explants were cultured on an induction medium (IM), which is a modified MS medium consisting of nitrate concentrations reduced by one quarter, 0.8 mg L−1 6-benzylaminopurine (BA) and 0.2 mg L−1 3-indole butyric acid (IBA). Explants were then transferred to a proliferation medium (PM) consisting of the same basal medium together with 0.03 mg L−1 BA and 0.05 mg L−1 gibberellic acid (GA3). A rooting double-phase was then established. The pre-rooting medium (PRM), consisting of a basal MS medium with half strength nitrate concentrations, 0.5 mg L−1 indole-3-acetic acid (IAA) and 1 mg L−1 paclobutrazol (PBZ) was used for two weeks. Over the next four weeks, a rooting medium (MR) was used, consisting of a basal MS medium with 2 mg L−1 β-cyclodextrin and 2 mg L−1 α-naphthaleneacetic acid sodium salt (NAA). The cv Empolese provided the highest number of proliferated explants and rooted plantlets using the method described.

Keywords: Cynara scolymus L.; Exponential growth curve; In vitro rooting; Paclobutrazol; Proliferation rate

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About the article

Published Online: 2012-06-03

Published in Print: 2012-08-01


Citation Information: Open Life Sciences, Volume 7, Issue 4, Pages 680–689, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-012-0064-x.

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© 2012 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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