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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz


IMPACT FACTOR 2018: 0.504
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2391-5412
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Volume 8, Issue 1

Issues

Volume 10 (2015)

A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols

Everaldo Attard
  • Institute of Earth Systems, Division of Rural Sciences and Food Systems, University of Malta, MSD 2080, Msida, Malta
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Published Online: 2012-11-21 | DOI: https://doi.org/10.2478/s11535-012-0107-3

Abstract

Several methods have been described for the determination of phenolic compounds in animal and plant products using the Folin-Ciocalteu (FC) assay. Most of these methods describe the use of this reagent and sodium carbonate in spectrophotometric methods. The macro FC assay was compared with two micro FC assays carried out on a microplate reader. Excellent correlation was obtained among the three assays with a molar extinction coefficient of 5.228±0.187x103 M−1 cm−1. The micro assay may serve as a high throughput method for the rapid determination of polyphenols in various samples.

Keywords: UV-VIS spectrophotometry; Microplate reader; Methanolic extracts; Tannic acid

  • [1] Häkkinen S., Heinonen M., Kärenlampi S., Mykkänen H., Ruuskanen J., Törrönen R., Screening of selected flavonoids and phenolic acids in 19 berries, Food Res Int, 1999, 32, 345–353 http://dx.doi.org/10.1016/S0963-9969(99)00095-2CrossrefGoogle Scholar

  • [2] Schieber A., Keller P., Carle R., Determination of phenolic acids and flavonoids of apple and pear by high-performance liquid chromatography, J Chrom., 2001, 910, 265–273 http://dx.doi.org/10.1016/S0021-9673(00)01217-6CrossrefGoogle Scholar

  • [3] Stratil P., Klejdus B., Kubáň V., Determination of Total Content of Phenolic Compounds and Their Antioxidant Activity in Vegetables — Evaluation of Spectrophotometric Methods, J Agric Food Chem., 2006, 54, 607–616 http://dx.doi.org/10.1021/jf052334jCrossrefGoogle Scholar

  • [4] Folin O., Ciocalteu V., On Tyrosine and Tryptophane Determinations in Proteins, J Biol Chem., 1927, 73, 627–650 Google Scholar

  • [5] Singhvi I., Goyal A., Visible spectrophotometric estimation of aceclofenac and indapamide from tablets using Folin-Cocalteu reagent, Indian J Pharmaceut Sci., 2007, 69, 164–165 http://dx.doi.org/10.4103/0250-474X.32140CrossrefGoogle Scholar

  • [6] Siddappa K., Metre M., Reddy T., Tambe, M., Gavanna M., Sensitive Spectrophotometric Methods for Quantitative Determination of Hydralazine Hydrochloride in Pure and Pharmaceutical Formulation, World J Chem., 2009, 4, 15–20 Google Scholar

  • [7] Singh D.K, Maheshwari G., Spectrophotometric determination of penicillins in pure and pharmaceutical formulations using Folin-Ciocalteu reagent, Drug Test Anal., 2010, 10, 503–506 http://dx.doi.org/10.1002/dta.157CrossrefGoogle Scholar

  • [8] Jagota S.K., Dani H.M., A new colorimetric technique for estimation of vitamin C using Folin phenol reagent, Anal Biochem., 1982, 127, 178–182 http://dx.doi.org/10.1016/0003-2697(82)90162-2CrossrefGoogle Scholar

  • [9] Waterhouse A.L., Determination of total phenolics, In: Wrolstad R.E. (Ed.), Current Protocols in Food Analytical Chemistry, John Wiley & Sons, New York, 2002 Google Scholar

  • [10] Medina-Remón A., Barrionuevo-González A., Zamora-Ros R., Andres-Lacueva C., Estruch R., Martínez-González M.-A., et al., Rapid Folin- Ciocalteu method using microtitre 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake, Anal Chim Acta., 2009, 634, 54–60 http://dx.doi.org/10.1016/j.aca.2008.12.012CrossrefGoogle Scholar

  • [11] Magalhães L.M., Santos F., Segundo M.A., Reis S., Lima J.L.F.C., Rapid microplate high-throughput methodology for assessment of Folin-Ciocalteu reducing capacity, Talanta., 2010, 83, 441–447 http://dx.doi.org/10.1016/j.talanta.2010.09.042CrossrefGoogle Scholar

  • [12] McDonald S., Prenzler P.D., Autolovich M., Robards K., Phenolic content and antioxidant activity of olive extracts, Food Chem., 2001, 73, 73–84 http://dx.doi.org/10.1016/S0308-8146(00)00288-0CrossrefGoogle Scholar

  • [13] Jung E.J., Bae M.S., Jo E.K., Jo Y.H., Lee S.C., Antioxidant activity of different parts of eggplant, J Med Plant Res., 2011, 18, 4610–4615 Google Scholar

  • [14] Ferreira I.C.F.R., Barros L., Soares M.E., Bastos M.L., Antioxidant activity and phenolic contents of Olea europaea L. leaves sprayed with different copper formulations, Food Chem., 2007, 103, 188–195 http://dx.doi.org/10.1016/j.foodchem.2006.08.006CrossrefGoogle Scholar

  • [15] Tzanakis E., Kalogeropoulos Th., Tzimas St., Chatzilazarou A., Katsoyannos E., Phenols and antioxidant activity of apple, quince, pomegranate, bitter orange and almond-leaved pear methanolic extracts, E-J Sci Tech., 2006, 1, 16–28 Google Scholar

  • [16] Abd El-aal H.A., Halaweish F.T., Food Preservative Activity of Phenolic Compounds in Orange Peel Extracts (Citrus sinensis L.), Lucrări Ştiinţifice., 2007, 53, 457–464 Google Scholar

  • [17] Magda R.A., Awad A.M., Selim K.A., Evaluation of Mandarin and Navel Orange. Peels as Natural Sources of Antioxidant in Biscuits, Alex J Pharm Sci., 2008, 75–82 Google Scholar

  • [18] Mangion Randon A., Attard E., The in vitro Immunomodulatory Activity of Oleuropein, a Secoiridoid Glycoside from Olea europaea L., Nat Prod Commun., 2007, 2, 515–519 Google Scholar

  • [19] Altıok E., Bayçın D., Bayraktar O., Ülkü S., Isolation of polyphenols from the extracts of olive leaves (Olea europaea L.) by adsorption on silk fibroin, Sep Purif Technol, 2008, 62, 342–348 http://dx.doi.org/10.1016/j.seppur.2008.01.022CrossrefGoogle Scholar

  • [20] Klimczak I., Malecka M., Szlachta M., Gliszczynska-Swiglo A., Effect of storage on the content of polyphenols, vitamin C and the antioxidant activity of orange juices, J Food Comp Anal., 2007, 20, 313–322 http://dx.doi.org/10.1016/j.jfca.2006.02.012CrossrefGoogle Scholar

  • [21] Olson R.M., Teo A., Singh A.P., Luthria D., Banuelos G.S., Pasakdee S.M., et al., Polyphenol content and antioxidant capacity of eggplant skin, FASEBJ., 2008, 22, 890.22 Google Scholar

  • [22] Beutler H.-O., L-Ascorbate and L-Dehydroascorbate; In: Bergmeyer H.U. (Ed.), Methods of Enzymatic Analysis (in Methods of Enzymatic Analysis) VCH Publishers (UK) Ltd., Cambridge, UK, 1984 Google Scholar

About the article

Published Online: 2012-11-21

Published in Print: 2013-01-01


Citation Information: Open Life Sciences, Volume 8, Issue 1, Pages 48–53, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-012-0107-3.

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© 2013 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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