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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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Volume 8, Issue 1


Volume 10 (2015)

A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols

Everaldo Attard
  • Institute of Earth Systems, Division of Rural Sciences and Food Systems, University of Malta, MSD 2080, Msida, Malta
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Published Online: 2012-11-21 | DOI: https://doi.org/10.2478/s11535-012-0107-3


Several methods have been described for the determination of phenolic compounds in animal and plant products using the Folin-Ciocalteu (FC) assay. Most of these methods describe the use of this reagent and sodium carbonate in spectrophotometric methods. The macro FC assay was compared with two micro FC assays carried out on a microplate reader. Excellent correlation was obtained among the three assays with a molar extinction coefficient of 5.228±0.187x103 M−1 cm−1. The micro assay may serve as a high throughput method for the rapid determination of polyphenols in various samples.

Keywords: UV-VIS spectrophotometry; Microplate reader; Methanolic extracts; Tannic acid

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About the article

Published Online: 2012-11-21

Published in Print: 2013-01-01

Citation Information: Open Life Sciences, Volume 8, Issue 1, Pages 48–53, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-012-0107-3.

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© 2013 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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