Abstract
Four experiments were carried out in 2010 and 2011 to determine how cultivation period (spring or autumn), harvest season (summer or autumn), and plant water status (irrigated or rainfed) influenced content and composition of broccoli cultivar Parthenon F1 with respect to sulforaphane and phenolics under field conditions in Gödöllő, Hungary. Sulforaphane content was significantly higher in the autumn harvests, regardless of irrigation treatments. Harvest season also influenced total phenolics content, with the highest values occurring in the spring season. Harvest season also affected trolox equivalent antioxidant capacity (TEAC) and this capacity was also the greatest in spring. Caffeic acid glucoside was a major phenolics component in both spring and autumn season harvests. The season and irrigation related changes in other phenolic component contents were also characterised in this study.
[1] Björkman T., Pearson K.J., High temperature arrest of inflorescence development in broccoli (Brassica oleracea var. italica L.), J. Expt. Bot., 1998, 49, 101–106 10.1093/jxb/49.318.101Search in Google Scholar
[2] Bohinc T., Ban S.G., Ban D., Trdan S., Glucosinolates in plant protection strategies: A review, Arch. Biol. Sci., 2012, 64, 821–828 http://dx.doi.org/10.2298/ABS1203821B10.2298/ABS1203821BSearch in Google Scholar
[3] Jahangir M., Abdel-Farid I.B., Kim H.K., Choi Y.H., Verpoorte R., Healthy and unhealthy plants: The effect of stress on the metabolism of Brassicaceae, Environ. Expt. Bot., 2009, 67, 23–33 http://dx.doi.org/10.1016/j.envexpbot.2009.06.00710.1016/j.envexpbot.2009.06.007Search in Google Scholar
[4] Jeffery E.H., Brown A.F., Kurilich A.C., Keck A.S., Matusheski N., Klein B.P., et al., Variation in content of bioactive components in broccoli, J. Food Compos. Anal., 2003, 16, 323–330 http://dx.doi.org/10.1016/S0889-1575(03)00045-010.1016/S0889-1575(03)00045-0Search in Google Scholar
[5] Herr I., Büchler M.W., Dietary constituents of broccoli and other cruciferous vegetables: Implications for prevention and therapy of cancer, Cancer Treatment Rev., 2010, 36, 377–383 http://dx.doi.org/10.1016/j.ctrv.2010.01.00210.1016/j.ctrv.2010.01.002Search in Google Scholar
[6] Gu Z.X., Guo Q.H., Gu Y.J., Factors Influencing Glucoraphanin and Sulforaphane Formation in Brassica Plants: A Review, J. Integr. Agric., 2012, 11, 1804–1816 http://dx.doi.org/10.1016/S2095-3119(12)60185-310.1016/S2095-3119(12)60185-3Search in Google Scholar
[7] Höglund A.S., Lenman M., Rask L., Myrosinase is localized to the interior of myrosin grains and is not associated to the surrounding tonoplast membrane, Plant Sci., 1992, 85, 165–170 http://dx.doi.org/10.1016/0168-9452(92)90112-Y10.1016/0168-9452(92)90112-YSearch in Google Scholar
[8] Hopkins R.J., van Dam N.M., van Loon J.J.A., Role of glucosinolates in insect-plant relationships and multitrophic interactions, Ann. Rev. Entomol., 2009, 54, 57–83 http://dx.doi.org/10.1146/annurev.ento.54.110807.09062310.1146/annurev.ento.54.110807.090623Search in Google Scholar PubMed
[9] Kliebenstein D.J., Kroymann J., Mitchell-Olds T., The glucosinolate-myrosinase system in an ecological and evolutionary context, Curr. Opin. Plant Biol., 2005, 8, 264–271 http://dx.doi.org/10.1016/j.pbi.2005.03.00210.1016/j.pbi.2005.03.002Search in Google Scholar PubMed
[10] Charron C.S., Saxton A.M., Sams C.E., Relationship of climate and genotype to seasonal variation in the glucosinolate-myrosinase system. I. Glucosinolate content in ten cultivars of Brassica oleracea grown in fall and spring seasons, J. Sci. Food Agric., 2005, 85, 671–681 http://dx.doi.org/10.1002/jsfa.188010.1002/jsfa.1880Search in Google Scholar
[11] Charron C.S., Saxton A.M., Sams C.E., Relationship of climate and genotype to seasonal variation in the glucosinolate-myrosinase system. II. Myrosinase activity in ten cultivars of Brassica oleracea grown in fall and spring seasons, J. Sci. Food Agric., 2005, 85, 682–690 http://dx.doi.org/10.1002/jsfa.203110.1002/jsfa.2031Search in Google Scholar
[12] Rosa E.A.S., Rodrigues A.S., Total and individual glucosinolate content in 11 broccoli cultivars grown in early and late seasons, Hortscience, 2001, 36, 56–59 10.21273/HORTSCI.36.1.56Search in Google Scholar
[13] Clarke J.D., Dashwood R.H., Ho E., Multi-targeted prevention of cancer by sulforaphane, Cancer Lett., 2008, 269, 291–304 http://dx.doi.org/10.1016/j.canlet.2008.04.01810.1016/j.canlet.2008.04.018Search in Google Scholar PubMed PubMed Central
[14] Dinkova-Kostova A.T., Jenkins S.N., Fahey J.W., Ye L., Wehage S.L., Liby K.T., et al., Protection against UV-light-induced skin carcinogenesis in SKH-1 high-risk mice by sulforaphane-containing broccoli sprout extracts, Cancer Lett., 2006, 240, 243–252 http://dx.doi.org/10.1016/j.canlet.2005.09.01210.1016/j.canlet.2005.09.012Search in Google Scholar PubMed
[15] Gills J.J., Jeffery E.H., Matusheski N.V., Moon R.C., Lantvit D.D., Pezzuto J.M., Sulforaphane prevents mouse skin tumorigenesis during the stage of promotion, Cancer Lett., 2006, 236, 72–79 http://dx.doi.org/10.1016/j.canlet.2005.05.00710.1016/j.canlet.2005.05.007Search in Google Scholar PubMed
[16] Guerrero-Beltrán C.E., Calderón-Oliver M., Pedraza-Chaverri J., Chirino Y.I., Protective effect of sulforaphane against oxidative stress: Recent advances, Exp. Toxicol. Pathol., 2012, 64, 503–508 http://dx.doi.org/10.1016/j.etp.2010.11.00510.1016/j.etp.2010.11.005Search in Google Scholar PubMed
[17] Guerrero-Beltrán C.E., Mukhopadhyay P., Horváth B., Rajesh M., Tapia E., García-Torres I., et al., Sulforaphane, a natural constituent of broccoli, prevents cell death and inflammation in nephropathy, J. Nutr. Biochem., 2012, 23, 494–500 http://dx.doi.org/10.1016/j.jnutbio.2011.02.00410.1016/j.jnutbio.2011.02.004Search in Google Scholar PubMed PubMed Central
[18] Anupama M., Murgan S.S., Balakrishna Murthy P., Broccoli flower head extract reduces mitomycin-C induced sister chromatid exchange in cultured human lymphocytes, Food Chem. Toxicol., 2008, 46, 3351–3353 http://dx.doi.org/10.1016/j.fct.2008.08.00910.1016/j.fct.2008.08.009Search in Google Scholar PubMed
[19] Brown A.F., Yousef G.G., Jeffery E.H., Klein B.P., Wallig M.A., Kushad M.M., et al., Glucosinolate profiles in broccoli: Variation in levels and implications in breeding for cancer chemoprotection, J. Amer. Soc. Hort. Sci., 2002, 127, 807–813 10.21273/JASHS.127.5.807Search in Google Scholar
[20] Vallejo F., Tomás-Barberán F.A., García-Viguera C., Effect of climatic and sulphur fertilisation conditions, on phenolic compounds and vitamin C, in the inflorescences of eight broccoli cultivars, Eur. Food Res. Technol., 2003, 216, 395–401 10.1007/s00217-003-0664-9Search in Google Scholar
[21] Pérez-Balibrea S., Moreno D.A., García-Viguera C., Genotypic effects on the phytochemical quality of seeds and sprouts from commercial broccoli cultivars, Food Chem., 2011, 125, 348–354 http://dx.doi.org/10.1016/j.foodchem.2010.09.00410.1016/j.foodchem.2010.09.004Search in Google Scholar
[22] Mewis I., Khan M.A.M., Glawischnig E., Schreiner M., Ulrichs C., Water Stress and Aphid Feeding Differentially Influence Metabolite Composition in Arabidopsis thaliana (L.), PLoS ONE, 2012, 7, e48661 http://dx.doi.org/10.1371/journal.pone.004866110.1371/journal.pone.0048661Search in Google Scholar PubMed PubMed Central
[23] Podsedek A., Natural antioxidants and antioxidant capacity of Brassica vegetables: A review, LWTFood Sci. Tech., 2007, 40, 1–11 10.1016/j.lwt.2005.07.023Search in Google Scholar
[24] Vallejo F., Tomás-Barberán F.A., Garcia-Viguera C., Glucosinolates and vitamin C content in edible parts of broccoli florets after domestic cooking, Eur. Food Res. Technol., 2002, 215, 310–316 http://dx.doi.org/10.1007/s00217-002-0560-810.1007/s00217-002-0560-8Search in Google Scholar
[25] Cartea M.E., Francisco M., Soengas P., Velasco P., Phenolic compounds in Brassica vegetables, Molecules, 2011, 16, 251–280 http://dx.doi.org/10.3390/molecules1601025110.3390/molecules16010251Search in Google Scholar PubMed PubMed Central
[26] Conaway C.C., Getahun S.M., Liebes L.L., Pusateri D.J., Topham D.K.W., Botero-Omary M., et al., Disposition of glucosinolates and sulforaphane in humans after ingestion of steamed and fresh broccoli, Nutr. Cancer, 2000, 38, 168–178 http://dx.doi.org/10.1207/S15327914NC382_510.1207/S15327914NC382_5Search in Google Scholar PubMed
[27] Gliszczyńska-Świgło A., Kałuzewicz A., Lemańska K., Knaflewski M., Tyrakowska B., The effect of solar radiation on the flavonol content in broccoli inflorescence, Food Chem., 2007, 100, 241–245 http://dx.doi.org/10.1016/j.foodchem.2005.09.04810.1016/j.foodchem.2005.09.048Search in Google Scholar
[28] Cogo S.L.P., Chaves F.C., Schirmer M.A., Zambiazi R.C., Nora L., Silva J.A., et al., Low soil water content during growth contributes to preservation of green colour and bioactive compounds of coldstored broccoli (Brassica oleraceae L.) florets, Postharvest Biol. Technol., 2011, 60, 158–163 http://dx.doi.org/10.1016/j.postharvbio.2010.12.00810.1016/j.postharvbio.2010.12.008Search in Google Scholar
[29] Fortier E., Desjardins Y., Tremblay N., Bélec C., Coté M., Influence of irrigation on broccoli polyphenolics concentration, Acta Hort., 2010, 856, 55–62 10.17660/ActaHortic.2010.856.6Search in Google Scholar
[30] Khan M.A.M., Ulrichs C., Mewis I., Water stress alters aphid-induced glucosinolate response in Brassica oleracea var. italica differently, Chemoecology, 2011, 21, 235–242 http://dx.doi.org/10.1007/s00049-011-0084-410.1007/s00049-011-0084-4Search in Google Scholar
[31] Khan M.A.M., Ulrichs C., Mewis I., Effect of water stress and aphid herbivory on flavonoids in broccoli (Brassica oleracea var. italica Plenck), J. Appl. Bot. Food Qual., 2011, 84, 178–182 Search in Google Scholar
[32] Sakata Vegetables Europe S.A.S., Parthenon F1, 2012 Search in Google Scholar
[33] Helyes L., Varga G., Irrigation demand of tomato according to the results of three decades, Acta Hort., 1994, 376, 323–328 10.17660/ActaHortic.1994.376.44Search in Google Scholar
[34] Nakagawa K., Umeda T., Higuchi O., Tsuzuki T., Suzuki T., Miyazawa T., Evaporative light-scattering analysis of sulforaphane in broccoli samples: Quality of broccoli products regarding sulforaphane contents, J. Agr. Food Chem., 2006, 54, 2479–2483 http://dx.doi.org/10.1021/jf051823g10.1021/jf051823gSearch in Google Scholar
[35] Liang H., Yuan Q.P., Dong H.R., Liu Y.M., Determination of sulforaphane in broccoli and cabbage by high-performance liquid chromatography, J. Food Compos. Anal., 2006, 19, 473–476 http://dx.doi.org/10.1016/j.jfca.2005.11.00510.1016/j.jfca.2005.11.005Search in Google Scholar
[36] Price K.R., Casuscelli F., Colquhoun I.J., Rhodes M.J.C., Hydroxycinnamic acid esters from broccoli florets, Phytochemistry, 1997, 45, 1683–1687 http://dx.doi.org/10.1016/S0031-9422(97)00246-X10.1016/S0031-9422(97)00246-XSearch in Google Scholar
[37] Brand-Williams W., Cuvelier M.E., Berset C., Use of a free radical method to evaluate antioxidant activity,. LWT — Food Sci. Technol., 1995, 28, 25–30 http://dx.doi.org/10.1016/S0023-6438(95)80008-510.1016/S0023-6438(95)80008-5Search in Google Scholar
[38] Robbins R.J., Keck A.S., Banuelos G., Finley J.W., Cultivation conditions and selenium fertilization alter the phenolic profile, glucosinolate, and sulforaphane content of broccoli, J. Med. Food, 2005, 8, 204–214 http://dx.doi.org/10.1089/jmf.2005.8.20410.1089/jmf.2005.8.204Search in Google Scholar PubMed
[39] Vallejo F., Garcia-Viguera C., Tomas-Barberan F.A., Changes in Broccoli (Brassica oleracea L. var. italica) health-promoting compounds within florescence development, J. Agr. Food Chem., 2003, 51, 3776–3782 http://dx.doi.org/10.1021/jf021233810.1021/jf0212338Search in Google Scholar PubMed
[40] Aires A., Fernandes C., Carvalho R., Bennett R.N., Saavedra M.J., Rosa E.A.S., Seasonal effects on bioactive compounds and antioxidant capacity of six economically important Brassica vegetables, Molecules, 2011, 16, 6816–6832 http://dx.doi.org/10.3390/molecules1608681610.3390/molecules16086816Search in Google Scholar PubMed PubMed Central
[41] Koh E., Wimalasiri K.M.S., Chassy A.W., Mitchell A.E., Content of ascorbic acid, quercetin, kaempferol and total phenolics in commercial broccoli, J. Food Compos. Anal., 2009, 22, 637–643 http://dx.doi.org/10.1016/j.jfca.2009.01.01910.1016/j.jfca.2009.01.019Search in Google Scholar
[42] Aldrich H.T., Kendall P., Bunning M., Stonaker F., Kulen O., Stushnoff C., Environmental temperatures influence antioxidant properties and mineral content in broccoli cultivars grown organically and conventionally, J. Agro Crop Sci., 2011, 2, 1–10 Search in Google Scholar
[43] Krumbein A., Saeger-Fink H., Schonhof I., Changes in quercetin and kaempferol concentrations during broccoli head ontogeny in three broccoli cultivars. J. Appl. Bot. Food Qual., 2007, 81, 136–139 Search in Google Scholar
[44] Soengas P., Cartea M.E., Francisco M., Sotelo T., Velasco P., New insights into antioxidant activity of Brassica crops, Food Chem., 2012, 134, 725–733 http://dx.doi.org/10.1016/j.foodchem.2012.02.16910.1016/j.foodchem.2012.02.169Search in Google Scholar PubMed
[45] Fernandez-Leon M.F., Fernandez-Leon A.M., Lozano M., Ayuso M.C., Gonzalez-Gomez D., Identification, quantification and comparison of the principal bioactive compounds and external quality parameters of two broccoli cultivars, J. Funct. Foods, 2012, 4, 465–473 http://dx.doi.org/10.1016/j.jff.2012.02.00510.1016/j.jff.2012.02.005Search in Google Scholar
[46] Nilsson J., Olsson K., Engqvist G., Ekvall J., Olsson, M., Nyman M., et al., Variation in the content of glucosinolates, hydroxycinnamic acids, carotenoids, total antioxidant capacity and lowmolecular-weight carbohydrates in Brassica vegetables, J. Sci. Food Agric., 2006, 86, 528–538 http://dx.doi.org/10.1002/jsfa.235510.1002/jsfa.2355Search in Google Scholar
[47] Vallejo F., Barberán F.A., Viguera G., Potential bioactive compounds in health promotion from broccoli cultivars grown in Spain, J. Sci. Food Agric., 2002, 82, 1293–1297 http://dx.doi.org/10.1002/jsfa.118310.1002/jsfa.1183Search in Google Scholar
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