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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz


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2391-5412
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Volume 9, Issue 2

Issues

Volume 10 (2015)

Mediated amperometry reveals two distinct modes of yeast responses to glucose

Rasa Garjonyte / Vytautas Melvydas / Algimantas Paškevičius / Valerijus Rašomavičius / Albertas Malinauskas
Published Online: 2013-11-09 | DOI: https://doi.org/10.2478/s11535-013-0257-y

Abstract

Mediated amperometry was exploited to monitor intracellular redox activity without cell disruption. Continuous measurements of menadione-mediated glucose currents at carbon paste electrodes with various immobilized intact wild type yeasts (Saccharomyces cerevisiae, Candida pulcherrima, Clavispora lusitaniae, Wickerhamomyces anomalus, Pichia guilliermondii, Kluyveromyces lactis var. lactis, Debaryomyces hansenii, Candida zeymolaydes and Candida tropicalis) revealed two distinct and previously unreported modes of development of the currents during the first 2 to 3 min. after subjection to glucose. A correlation among the values of the currents and the capacities of wild type yeasts to secrete various substances was observed.

Keywords: Yeasts; Menadione; Mediated amperometry; Carbon paste; Glucose

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About the article

Published Online: 2013-11-09

Published in Print: 2014-02-01


Citation Information: Open Life Sciences, Volume 9, Issue 2, Pages 173–181, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-013-0257-y.

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