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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz


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Volume 9, Issue 2

Issues

Volume 10 (2015)

Toxigenic Fusarium species infecting wheat heads in Poland

Halina Wiśniewska / Łukasz Stępień
  • Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, 60-479, Poznań, Poland
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/ Agnieszka Waśkiewicz / Monika Beszterda / Tomasz Góral / Jolanta Belter
Published Online: 2013-11-09 | DOI: https://doi.org/10.2478/s11535-013-0262-1

Abstract

Toxigenic Fusarium species are common pathogens of wheat and other cereals worldwide. In total, 449 wheat heads from six localities in Poland, heavily infected with Fusarium during 2009 season, were examined for Fusarium species identification. F. culmorum was the most common species (72.1% on average) with F. graminearum and F. avenaceum the next most commonly observed, but much less frequent (13.4 and 12.5% respectively). F. cerealis was found in 1.8% of all samples, and F. tricinctum was found only in one sample (0.2%). Subsequent quantification of the three major mycotoxins (deoxynivalenol, zearalenone and moniliformin) in grain and chaff fractions with respect to associated prevailing pathogen species uncovered the following patterns. Moniliformin (MON) was found in low amounts in all samples with F. avenaceum present. In contrast, deoxynivalenol (DON) and zearalenone (ZEA) were the contaminants of F. culmorum- and F. graminearum-infected heads. The highest concentration of DON was recorded in grain sample collected in Radzików (77 µg g−1). High temperatures in Central Poland during July and August accompanied with high rainfall in July were responsible for this high DON accumulation. Trichothecene, zearalenone, enniatin and beauvericin chemotypes were identified among 21 purified isolates using gene-specific PCR markers.

Keywords: Deoxynivalenol; DNA markers; Fusarium head blight; Moniliformin; Mycotoxins; Zearalenone

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About the article

Published Online: 2013-11-09

Published in Print: 2014-02-01


Citation Information: Open Life Sciences, Volume 9, Issue 2, Pages 163–172, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-013-0262-1.

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