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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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2391-5412
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Volume 10, Issue 1 (Nov 2014)

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Isolation and preliminary characterization of cyanobacteria strains from freshwaters of Greece

Spyros Gkelis
  • Department of Botany, School of Biology, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Macedonia, Greece
  • Email:
/ Pablo Fernández Tussy
  • Department of Botany, School of Biology, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Macedonia, Greece
/ Nikos Zaoutsos
  • Department of Botany, School of Biology, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Macedonia, Greece
Published Online: 2014-11-20 | DOI: https://doi.org/10.1515/biol-2015-0006

Abstract

Cyanobacterial harmful algal blooms (or CyanoHABs) represent one of the most conspicuous waterborne microbial hazards. The characterization of the bloom communities remains problematic because the cyanobacterial taxonomy of certain genera has not yet been resolved. In this study, 29 planktic and benthic cyanobacterial strains were isolated from freshwaters located in Greece. The strains were assigned to the genera Chroococcus, Microcystis, Synechococcus, Jaaginema, Limnothrix, Pseudanabaena, Anabaena, and Calothrix and screened for the production of the cyanotoxins microcystins (MCs), cylindrospermopsins (CYNs), and saxitoxins (STXs) using molecular (PCR amplification of seven genes implicated in cyanotoxin biosynthesis) and immunological (ELISA) methods. This study presents, for the first time, a cyanobacteria culture collection from Greece, thus providing missing study material for the understanding of bloom formation and cyanotoxin production in the Mediterranean and for the polyphasic characterization of important components of the phytoplankton. The combined use of molecular and immunochemical methods allowed the identification of MC producing strains, but further data are needed for CYNand STX-producing cyanobacteria. The high percentage of MC-producing Microcystis strains in the urban Lakes Kastoria and Pamvotis, frequently used for agriculture irrigation, fishing and recreation, highlights the potential risk for human health.

Keywords: Microcystis; Anabaena; Limnothrix; Calothrix; cyanotoxins; molecular detection; lakes; ELISA

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About the article

Received: 2014-01-28

Accepted: 2014-08-21

Published Online: 2014-11-20



Citation Information: Open Life Sciences, ISSN (Online) 2391-5412, DOI: https://doi.org/10.1515/biol-2015-0006. Export Citation

©2015 Spyros Gkelis et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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