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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz


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Volume 10, Issue 1

Issues

Volume 10 (2015)

Testing antiplatelet and antioxidant activity of the extract of seven varieties of Allium cepa L.

Aniela Saplonţai-Pop
  • Faculty of General Medicine, Department of Cardiology, “Iuliu Haţieganu” University of Medicine and Pharmacy, 8 Victor Babeș street, Cluj-Napoca, 400012, Romania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Augustin Moţ
  • Department of Chemistry and Chemical Engeneering, “Babeș- Bolyai” University, 1 Mihail Kogălniceanu street, Cluj-Napoca, 400084, Romania
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  • De Gruyter OnlineGoogle Scholar
/ Marioara Moldovan
  • Department of Polymeric Composites, “Raluca Rîpan” Institute of Chemistry, 30 Fântânele street, Cluj-Napoca, 400294, Romania
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  • De Gruyter OnlineGoogle Scholar
/ Radu Oprean
  • Department of Analytical Chemistry, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 Louis Pasteur street, Cluj- Napoca, 400349, Romania
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  • De Gruyter OnlineGoogle Scholar
/ Radu Silaghi-Dumitrescu
  • Department of Chemistry and Chemical Engeneering, “Babeș- Bolyai” University, 1 Mihail Kogălniceanu street, Cluj-Napoca, 400084, Romania
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  • De Gruyter OnlineGoogle Scholar
/ Olga Orășan
  • 4th Medical Clinic, “Iuliu Haţieganu” University of Medicine and Pharmacy, 16-20 Republicii street, Cluj-Napoca, 400015, Romania
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  • De Gruyter OnlineGoogle Scholar
/ Marcel Pârvu
  • Department of Biology, “Babeș-Bolyai” University, 42 Gh. Bilascu street, Cluj-Napoca, 400015, Romania
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/ Emese Gal
  • Department of Chemistry and Chemical Engeneering, “Babeș- Bolyai” University, 1 Mihail Kogălniceanu street, Cluj-Napoca, 400084, Romania
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  • De Gruyter OnlineGoogle Scholar
/ Corina Ionescu
  • Department of Biochemistry, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 Louis Pasteur street, Cluj- Napoca, 400349, Romania
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Published Online: 2015-02-04 | DOI: https://doi.org/10.1515/biol-2015-0011

Abstract

Background: The extracts of Allium cepa are known for their medical use: antioxidant, antiinflammatory, antimicrobial, fibrinolytic and antiplatelet properties. Our study aims to establish, using in vitro tests, the antiplatelet and antioxidant character, the link between them and the extract acidity, from seven varieties of A. cepa. Methodology: The qualitative and quantitative presence of polyphenols and anthocyanins in the extracts was determined using UV-Vis and HPLC. Quantitative determination of the thiosulfinates compounds was calculated using their reaction with 4-mercaptopyridine. Antioxidant character was determined using 3 methods (FC, DPPH and TEAC), and antiplatelet effect was measured by in vitro tests on platelet rich plasma obtained from human blood. Principal Findings/Results: The white variety of A. cepa has the most alkaline pH, the largest amount of thiosulfinate compounds and the most powerful antiplatelet effect, but a very small amount of flavonoids and an antioxidant effect almost nonexistent, in contrast with red variety of A. cepa which is the opposite. Conclusions/Significance: The white variety of A. cepa had very high anitiplatelet activity suggesting the potential use of A. cepa extract in treating cardiovascular diseases.

This article offers supplementary material which is provided at the end of the article.

Keywords: antiplatelet activity; antioxidant activity; Allium cepa; pH; thiosulfinates

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About the article

Received: 2014-04-07

Accepted: 2014-07-21

Published Online: 2015-02-04


Citation Information: Open Life Sciences, Volume 10, Issue 1, ISSN (Online) 2391-5412, DOI: https://doi.org/10.1515/biol-2015-0011.

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©2015 Aniela Saplonţai-Pop et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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