Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz


IMPACT FACTOR 2017: 0.764
5-year IMPACT FACTOR: 0.787

CiteScore 2017: 0.88

SCImago Journal Rank (SJR) 2017: 0.271
Source Normalized Impact per Paper (SNIP) 2017: 0.545

ICV 2017: 154.48

Open Access
Online
ISSN
2391-5412
See all formats and pricing
More options …
Volume 10, Issue 1

Issues

Volume 10 (2015)

Application of chosen factors in the wide crossing method for the production of oat doubled haploids

Agata Nowakowska
  • The F. Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Cracow, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Edyta Skrzypek
  • The F. Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Cracow, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Izabela Marcińska
  • The F. Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Cracow, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ilona Czyczyło-Mysza
  • The F. Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Cracow, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Kinga Dziurka
  • The F. Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Cracow, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Katarzyna Juzoń
  • The F. Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Cracow, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Katarzyna Cyganek
  • The F. Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Cracow, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Marzena Warchoł
  • The F. Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Cracow, Poland
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-02-27 | DOI: https://doi.org/10.1515/biol-2015-0014

Abstract

Oat (Avena sativa L.) has recently gained importance due to the discovery of a variety of health benefits and new opportunities of use. There is no efficient protocol for the production of oat doubled haploid (DH) lines. The aim of this study was to increase the efficiency of obtaining DHs of oat by the wide crossing method. The study was performed on five oat genotypes. We have compared the induction of embryos after pollination with maize, sorghum and millet pollen as well as the development of haploid embryos isolated 2, 3 and 4 weeks after pollination and cultivated on media with different sugar content. Haploid plants were treated with colchicine after or before acclimation to natural conditions. Of the three types of pollen used, the largest number of haploid embryos was obtained using maize pollen. Three weeks after pollination was the most suitable time for the isolation and cultivation of the embryos. The most efficient medium enabling the development of embryos and conversion to plants was 190-2 containing 9% of maltose. Colchicine treatment of acclimated plants provided high survival rate.

Keywords: Doubled haploids; Avena sativa L.; Pollen donor; Maltose; Embryo development

References

  • [1] Peterson D.M., Oat – a multifunctional grain, In: P. Peltonen- Saino, M. Topi-Hulmi (Eds.), Proceedings of 7th International Oat Conference, Helsinki, Finland, Agrifood Research Reports, 2004, 51, 21-26 Google Scholar

  • [2] Rines H.W., Molnar S.J., Tinker N.A., Philips R.L., Oat, In: C. Kole (Ed.), Genome Mapping and Molecular Breeding in Plants, Vol. 1 Cereals and Millets, Springer-Verlag Berlin Heidelberg, 2006, 211-242 Google Scholar

  • [3] Kiviharju E., Moisander S., Laurila J., Improved green plant rates from oat anther culture and the agronomic performance of some DH lines, Plant Cell Tiss. Organ Cult., 2005, 81, 1-9 CrossrefGoogle Scholar

  • [4] Rines H.W., Dahleen L.S., Haploid oat plants produced by application of maize pollen to emasculated oat florets, Crop Sci., 1990, 30, 1073-1078 CrossrefGoogle Scholar

  • [5] Sidhu P.K., Howes N.K., Aung T., Zwer P.K., Davies A., Factors affecting oat haploid production following oat x maize hybridization, Plant Breed., 2006, 125, 243-247 Google Scholar

  • [6] Aung T., Development and use of doubled haploids in oats, In: J. Chong (Ed.), Oat Newsletter 44, 1998 Google Scholar

  • [7] Matzk F., Hybrids of Crosses between oat and Andropogoneae or Paniceae species, Crop Sci., 1996, 36, 17-21 CrossrefGoogle Scholar

  • [8] Okagaki R.J., Kynast R.G., Livingston S.M., Russell C.D., Rines H.W., Phillips R.L., Mapping maize sequences to chromosomes using oat-maize chromosome addition materials, Plant Physiol., 2001, 125(3), 1228-1235 CrossrefGoogle Scholar

  • [9] Ishii T.T. , Ueda H.T, Tsujimoto H., Chromosome elimination by wide hybridization between Triticeae or oat plant and pearl millet: pearl millet chromosome dynamics in hybrid embryo cells, Chromosome Res., 2010, 18 (7), 821-831 CrossrefGoogle Scholar

  • [10] Marcińska I., Nowakowska A., Skrzypek E., Czyczyło-Mysza I., Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.), Cent. Eur. J. Biol., 2013, 8(3), 306-313 Google Scholar

  • [11] Hoagland D.R., Arnon D.I., A water culture method for growing plants without soil, Circ. Univ. Calif., Agric. Exp. Stn., 1938, No. 347 Google Scholar

  • [12] Wang X.Z., Hu H., The effect of potato II medium for triticale anther culture, Plant Sci. Lett., 1984, 36, 237-239 CrossrefGoogle Scholar

  • [13] Murashige T., Skoog F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 1962, 15, 473-497 Google Scholar

  • [14] Galbraith D.W., Harkins K.R., Maddox J.M., Ayres N.M., Sharma D.P., Firoozabady E. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science, 1983, 220, 1049-1051 Google Scholar

  • [15] Wędzony M., Forster B.P., Żur I., Golemiec E., Szechyńska-Hebda M., Dubas E., Gołębiowska G., Progress in doubled Haploid Technology in Higher Plants, In: A. Touraev, B.P. Forster, S. M. Jain (Eds.), Advances in Haploid Production in Higher Plants, Springer, 2009, 1-33 Google Scholar

  • [16] Norstog K., New synthetic medium for the culture of premature barley embryos, In Vitro 1973, 8, 307-308 Google Scholar

  • [17] Tiara T., Larter E.N., Factors influencing development of wheat-rye hybrid embryos in vitro, Crop Sci., 1978, 18, 348-350 CrossrefGoogle Scholar

  • [18] Kiviharju E., Puolimatka M., Saastamoinen M., Pehu E., Extension of anther culture to several genotypes of cultivated oats, Plant Cell Rep., 2000, 19, 74–679 CrossrefGoogle Scholar

  • [19] Nowakowska A., Optimization of method for oat (Avena sativa L.) doubled haploid production (in Polish), 2012, PhD thesis, pp. 129 Google Scholar

About the article

Received: 2014-06-06

Accepted: 2014-09-19

Published Online: 2015-02-27


Citation Information: Open Life Sciences, Volume 10, Issue 1, ISSN (Online) 2391-5412, DOI: https://doi.org/10.1515/biol-2015-0014.

Export Citation

©2015 Agata Nowakowska et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Edyta Skrzypek, Tomasz Warzecha, Angelika Noga, Marzena Warchoł, Ilona Czyczyło-Mysza, Kinga Dziurka, Izabela Marcińska, Kamila Kapłoniak, Agnieszka Sutkowska, Zygmunt Nita, Krystyna Werwińska, Dominika Idziak-Helmcke, Magdalena Rojek, and Marta Hosiawa-Barańska
PeerJ, 2018, Volume 6, Page e5107

Comments (0)

Please log in or register to comment.
Log in