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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz


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2391-5412
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Volume 10, Issue 1

Issues

Volume 10 (2015)

In vitro plant regeneration of lotus (Nelumbo nucifera)

Xia Yu
  • State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Luojia Hill, Wuhan, Hubei, 430072, P.R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jiajing Sheng
  • State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Luojia Hill, Wuhan, Hubei, 430072, P.R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lingling Zhao
  • State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Luojia Hill, Wuhan, Hubei, 430072, P.R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ying Diao
  • State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Luojia Hill, Wuhan, Hubei, 430072, P.R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Xingwen Zheng / Keqiang Xie / Mingquan Zhou / Zhongli Hu
  • State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Luojia Hill, Wuhan, Hubei, 430072, P.R. China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-03-13 | DOI: https://doi.org/10.1515/biol-2015-0016

Abstract

In this study, an efficient and reproducible plant regeneration system for lotus (Nelumbo nucifera Gaertn.) was established using shoot apical meristems from the buds and one-week-old aseptically germinated embryos as explants. Multiple shoot clumps were induced on Murashige and Skoog (MS) basal medium supplemented with various combinations of N6-Benzylaminopurine (6-BA) and α-Naphthalene acetic acid (NAA). The maximum response was obtained with 2.22 μM 6-BA, and produced 21.33 shoots per explant after four weeks. After five subcultures, multiple shoot clumps were transferred to MS basal medium supplemented with various combinations of 3-Indolebutyric acid (IBA), NAA and sucrose for root induction. After four weeks, plantlets with well-developed roots were achieved on MS basal medium supplemented with 0.54 μM NAA and 30 g/L sucrose with 100% rooting rate. The successfully acclimated plantlets were transferred to pots with the addition of 2 g/L KMnO4 into the soils, and finally fertile plants with much bigger leaves were obtained in the greenhouse. The survival rate was 97.33%.

Keywords: Shoot apical meristem; Multiple shoot clumps induction; Root induction; Plant regeneration

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About the article

Received: 2014-06-23

Accepted: 2014-08-24

Published Online: 2015-03-13


Citation Information: Open Life Sciences, Volume 10, Issue 1, ISSN (Online) 2391-5412, DOI: https://doi.org/10.1515/biol-2015-0016.

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©2015 Xia Yu et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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