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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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Volume 10, Issue 1


Volume 10 (2015)

Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil

Anca Hermenean
  • Department of Histology, Faculty of Medicine, Pharmacy and Dentistry, Vasile Goldis Western University of Arad, Romania
  • Institute of Life Sciences, Vasile Goldis Western University of Arad, Romania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Miruna Stan / Aurel Ardelean / Luminița Pilat
  • Department of Biochemistry, Faculty of Medicine, Pharmacy and Dentistry, Vasile Goldis Western University of Arad, Romania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ciprian Valentin Mihali / Cristina Popescu
  • Institute of Life Sciences, Vasile Goldis Western University of Arad, Romania
  • Department of Pharmaceutical Sciences, Faculty of Medicine, Pharmacy and Dentistry, Vasile Goldis Western University of Arad, Romania
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lajos Nagy / György Deák / Miklós Zsuga / Sándor Kéki / Ildikó Bácskay / Ferenc Fenyvesi / Marieta Costache / Anca Dinischiotu / Miklós Vecsernyés
Published Online: 2015-04-02 | DOI: https://doi.org/10.1515/biol-2015-0017


This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.

Keywords: Silybum marianum; seed oil; CCl4; oxidative stress; antioxidant; hepatoprotection


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About the article

Received: 2014-03-04

Accepted: 2014-08-20

Published Online: 2015-04-02

Citation Information: Open Life Sciences, Volume 10, Issue 1, ISSN (Online) 2391-5412, DOI: https://doi.org/10.1515/biol-2015-0017.

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©2015 Anca Hermenean et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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