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Volume 62, Issue 1


Isolation of pathogenic bacteria from Oberea linearis (Coleptera: Cerambycidae)

Ali Bahar / Zihni Demirbağ
Published Online: 2007-02-01 | DOI: https://doi.org/10.2478/s11756-007-0009-4


The bacterial flora of the Oberea linearis (Coleoptera: Cerambycidae) was investigated and 13 different bacteria were isolated from O. linearis larvae. Seven of these bacteria were performed and characterized at species level and the rest of them were characterized at genus level. In this study, we determined morphological and physiological characteristics of the bacterial isolates by conventional and routine techniques, biochemical properties and metabolic enzyme profiles by API20E and Phoenix 1000A panel test systems. Additionally, 16S rRNA gene sequence analysis was also performed to identify the isolates at the molecular level. The isolates were identified as Acinetobacter calcoaceticus (Ol1), Enterobacter aerogenes (Ol2), Pseudomonas sp. (Ol3), Flavobacterium sp. (Ol4), Microbacterium sp. (Ol5), Enterobacter agglomerans (Ol6), Xanthomonas sp. (Ol7), Pseudomonas syringae (Ol8), Pseudomonas sp. (Ol9), Xanthomonas sp. (Ol10), Enterobacter cancerogenus (Ol11), Xanthomonas maltophilia (Ol12), and Serratia marcescens (Ol13). This is the first record of bacterial isolates (Ol5, Ol8, Ol11, Ol12) from any insect. All these bacteria were tested against O. linearis larvae, and Serratia marcescens was found to cause the highest mortality (65%). On the other hand, we determined 90% mortality against this pest within four days by utilizing spore and crystal mixture of Bacillus thuringiensis isolated from Melolontha melolontha.

Keywords: Oberea linearis; bacterial flora; microbial control; insect pathology

  • [1] Alsina M. & Blanch A.R. 1994. A set of keys for biochemical identification of environmental Vibrio species. J. Appl. Bacteriol. 76: 79–85. CrossrefGoogle Scholar

  • [2] Altschul S.F., Gish W., Miller W., Myers E.W. & Lipman D.J. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403–410. http://dx.doi.org/10.1006/jmbi.1990.9999CrossrefGoogle Scholar

  • [3] Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W. & Lipman D.J. 1997. Gapped BLAST and PSIBLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389–3402. http://dx.doi.org/10.1093/nar/25.17.3389CrossrefGoogle Scholar

  • [4] Anonymous. 1995. Zirai Mucadele Teknik Talimatlari, Cilt 3. Tarim ve Koyisleri Bakanligi, Ankara. Google Scholar

  • [5] Aptosoglou S.G., Sivropoulou A. & Koliais S.I. 1997. Plasmid patterns of Bacillus thuringiensis strains and isolates. Microbios 91: 203–214. PubMedGoogle Scholar

  • [6] Bucher G.E. 1967. Pathogens of tobacco and tomato hornworms. J. Invertebr. Pathol. 9: 82–89. http://dx.doi.org/10.1016/0022-2011(67)90047-XCrossrefGoogle Scholar

  • [7] Case C.L. & Johnson T.R. 1992. Laboratory Experiments in Microbiology, 3rd Ed. Benjamin Cummings Publishing Company, Inc., California, 350 pp. Google Scholar

  • [8] Demir I., Sezen K. & Demirbag Z. 2002. The first study on bacterial flora and biological control agent of Anoplus roboris (Sufr., Coleoptera). J. Microbiol. 40: 104–108. Google Scholar

  • [9] Hurst G.D.D., Bandi C., Sacchi L., Cochrane A.G., Bertrand D., Karaca I. & Majerus M.E.N. 1999. Adonia variegata (Coleoptera: Coccinellidae) bears maternally inherited Flavobacteria that kill males only. Parasitology 118: 125–134. http://dx.doi.org/10.1017/S0031182098003655CrossrefGoogle Scholar

  • [10] Jackson T.A., Boucias D.G. & Thaler J.O. 2001. Pathobiology of amber disease, caused by Serratia spp., in the New Zeland grass grub, Costelytra zealandica. J. Invertebr. Pathol. 78: 232–243. http://dx.doi.org/10.1006/jipa.2002.5078CrossrefGoogle Scholar

  • [11] Jeyaprakash A., Hoy M.A. & Allsopp M.H. 2003. Bacterial diversity in worker adults of Apis mellifera capensis and Apis mellifera scutellata (Insecta: Hymenoptera) assessed using 16S rRNA sequences. J. Invertebr. Pathol. 84: 96–103. http://dx.doi.org/10.1016/j.jip.2003.08.007CrossrefGoogle Scholar

  • [12] Krieg N.R. & Holt J.G. 1986. Gram-negative aerobic rods and cocci, pp. 140–218. In: Palleroni N.J. (ed.) Bergey’s Manual of Systematic Bacteriology, Vol. 1, Williams and Wilkins, Baltimore. Google Scholar

  • [13] Kuzina L.V., Peloquin J.J., Vacek D.C. & Miller T.A. 2001. Isolation and identification of bacteria associated with adult laboratory Mexican fruit flies, Anastrepha ludens (Diptera: Tephritidae). Curr. Microbiol. 42: 290–294. Google Scholar

  • [14] Lipa J.J. & Wiland E. 1972. Bacteria isolated from cutworms and their infectivity to Agrotis sp. Acta Microbiol. Polonica Ser. B 4: 127–140. Google Scholar

  • [15] Lynch R.E., Lewis L.C. & Brindley T.A. 1976. Bacteria associated with eggs and first-instar larvae of the European corn borer: identification and frequency of occurrence. J. Invertebr. Pathol. 27: 229–237. http://dx.doi.org/10.1016/0022-2011(76)90150-6CrossrefGoogle Scholar

  • [16] Milenkovic S. & Mitrovic M. 2001. Hazelnut pests in Serbia. Acta Horticulturae 556: 403–406. Google Scholar

  • [17] Moar W.J., Pusztzai-Carey M. & Mack T.P. 1995. Toxicity of purified proteins and the HD-1 strain from Bacillus thuringiensis againts lesser cornstalk borer (Lepidoptera: Pyralidae). J. Econ. Entomol. 88: 606–609. CrossrefGoogle Scholar

  • [18] Osborn F., Berlioz L., Vitelli-Flores J., Monsalve W., Dorta B. & Lemoine V.R. 2002. Pathogenic effects of bacteria isolated from larvae of Hylesia metabus crammer (Lepidoptera: Saturniidae). J. Invertebr. Pathol. 80: 7–12. http://dx.doi.org/10.1016/S0022-2011(02)00037-XCrossrefGoogle Scholar

  • [19] Poinar G.O. & Thomas G.M. 1978. Diagnostic Manual for the Identification of Insect Pathogens. Plenum Press, New York, 218 pp. Google Scholar

  • [20] Sambrook J., Fritsch E.F. & Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor. Google Scholar

  • [21] Sandra W.W. & Douglas I.G. 2004. Microorganisms associated with field-collected Chrysoperla rufilabris (Neuroptera: Chrysopidae) adults with emphasis on yeast symbionts. Biol. Control 29: 155–168. http://dx.doi.org/10.1016/S1049-9644(03)00139-7CrossrefGoogle Scholar

  • [22] Sezen K. & Demirbag Z. 1999. Isolation and insecticidal activity of some bacteria from the hazelnut beetle (Balaninus nucum L.). Appl. Entomol. Zool. 34: 85–89. Google Scholar

  • [23] Sezen K., Demir I., Kati H. & Demirbag Z. 2005. Investigations on bacteria as a potential biological control agent of summer chafer, Amphimallon solstitiale L. (Coleoptera: Scarabaeidae). J. Microbiol. 43: 463–468. Google Scholar

  • [24] Sezen K., Yaman M. & Demirbag Z. 2001. Insecticidal potential of Serratia marcescens Bn10. Biologia 56: 333–336. Google Scholar

  • [25] Sneath P.H.A., Mair N.S., Sharpe M.E. & Holt J.G. 1986. Regular, nonsporing Grampositive rods, pp. 1208–1260. In: Kandler O. & Weiss N. (eds) Bergey’s Manual of Systematic Bacteriology, Vol. 2, Williams and Wilkins, Baltimore. Google Scholar

  • [26] Sramova H., Daniel M., Absolonova V., Dedicova D., Jedlickova Z., Lhotova H., Petras P. & Subertova V. 1992. Epidemiological role of arthropods detectable in health facilities. J. Hosp. Infect. 20: 281–292. http://dx.doi.org/10.1016/0195-6701(92)90006-8CrossrefGoogle Scholar

  • [27] Thiery I. & Frachon E. 1997. Idendification, isolation, culture and preservation of enthomopathogenic bacteria, pp. 55–73. In: Lacey A.L. (ed.) Manual of Techniques in Insect Pathology, Academic Press, London. Google Scholar

  • [28] William G.W., Susan M.B., Dale A.P. & David J.L. 1991. 16S Ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173: 697–703. Google Scholar

About the article

Published Online: 2007-02-01

Published in Print: 2007-02-01

Citation Information: Biologia, Volume 62, Issue 1, Pages 13–18, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-007-0009-4.

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© 2007 Institute of Molecular Biology, Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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