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Volume 62, Issue 3

Issues

Chlorophyll a extraction from freshwater algae — a reevaluation

Michael Schagerl / Gabriela Künzl
Published Online: 2007-06-01 | DOI: https://doi.org/10.2478/s11756-007-0048-x

Abstract

In this study, two extracting methods (sonication and dispersing) and three solvents (90% acetone, N,N′-dimethylformamide and methanol) were compared for their ability to extract chlorophyll a of freshwater phytoplankton. Measurements were performed with both spectrophotometry and high-performance liquid chromatography. Results showed that (i) cell disruption is essential and that (ii) the method of cell disruption and solvent applied differed significantly. Dispersing in acetone surpassed all other combinations. Sonication in N,N′-dimethylformamide was found less effective. N,N′-dimethylformamide and methanol seem to promote the formation of degradation products (chlorophyllide a, allomer, epimer and phaeophytin a) which lead to overestimates of chlorophyll a of about 10% by means of spectrophotometry.

Keywords: chlorophyll a; extraction; phytoplankton; HPLC; spectrophotometry; sonication

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About the article

Published Online: 2007-06-01

Published in Print: 2007-06-01


Citation Information: Biologia, Volume 62, Issue 3, Pages 270–275, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-007-0048-x.

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© 2007 Institute of Botany, Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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