Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biologia




More options …
Volume 62, Issue 3

Issues

Effect of vitamin E and selenium on antioxidant enzymes in brain, kidney and liver of cigarette smoke-exposed mice

Aysun Ozkan / Kayahan Fiskin / Ayse Ayhan
Published Online: 2007-06-01 | DOI: https://doi.org/10.2478/s11756-007-0060-1

Abstract

The present study was conducted to evaluate the protective effects of vitamin E and selenium (Se) application on alteration of antioxidant enzyme activities against cigarette smoking induced oxidative damage in brains, kidneys and liver of mice. Male mice (balb/c) were exposed to cigarette smoke and treated with Se and/or vitamin E. Glutathione transferase (GST), glutathione peroxidase (GPX), glutathione reductase (GRX), superoxide dismutase (SOD) and catalase (CAT) enzyme activities in mice brain, kidney and liver were measured spectrophotometrically. GST, GPX, GRX, SOD and CAT enzyme activities in the brains of smoke-exposed mice were found lower than the enzymes activities of control mice and Se-and vitamin E-treated mice at the end of the three and five months. Opposite to brain, enzyme activities in kidneys and livers of smoke-exposed mice were found higher than the enzymes activities of control mice and Se-and vitamin E-treated mice at the end of the three and five months. Activities of GST, GPX, GRX SOD and CAT in the livers, kidneys and brains of smoke-exposed mice were found statistically different (p < 0.01) compared to control mice and Se-and vitamin E-treated mice. Combined application of vitamin E and Se had an additive protective effect against changing enzymes activities in smoke-exposed mice livers, kidneys and brains at the end of the both application periods. These results suggest that cigarette smoke exposure enhances the oxidative stress, thereby disturbing the tissue antioxidant defense system and combined application of vitamin E and Se protects the brain, kidney and liver from oxidative damage through their antioxidant potential.

Keywords: mice (balb/c); smoking; antioxidants

  • [1] Anbarasi K., Vani G., Balakrishna K. & Shyamala Devi C.S. 2006. Effect of bacoside A on brain antioxidant status in cigarette smoke exposed rats. Life Sci. 78: 1378–1384. http://dx.doi.org/10.1016/j.lfs.2005.07.030CrossrefGoogle Scholar

  • [2] Azzi Montecucco C. & Richter C. 1975. The use of acetylated ferricytochrome c for the detection of superoxide radicals produced in biological membranes. Biochem. Biophys. Res. Commun. 65: 597–603. http://dx.doi.org/10.1016/S0006-291X(75)80188-4CrossrefGoogle Scholar

  • [3] Baskaran S., Lakshmi S. & Prasad P.R. 1999. Effect of cigarette smoke on lipid peroxidation and antioxidant enzymes in albino rat. Ind. J. Exp. Biol. 37: 1996–1200. Google Scholar

  • [4] Brown K.M., Morrice P.C. & Duthie G.G. 1997. Erythrocyte vitamin E and plasma ascorbate concentrations in relation to erythrocyte peroxidation in smokers and non-smokers: dose response to vitamin E supplementation. Am. J. Clin. Nutr. 65: 496–502. Google Scholar

  • [5] Calberg I. & Mannervik B. 1985. Glutathione reductase. Methods Enzymol. 113: 484–499. Google Scholar

  • [6] Claycombe H.J. & Meydani S.N. 2001. Vitamin E and genome stability. Mutat. Res. 475: 37–44. Google Scholar

  • [7] El-Bayoumy K. 2001. The protective role of selenium on genetic damage and cancer. Mutat. Res. 475: 123–139. Google Scholar

  • [8] Flohe L. & Gunzler W.A. 1984. Glutathione peroxidase. Methods Enzymol. 105: 115–121. Google Scholar

  • [9] Greenwald P., Milner J.A., Anderson E.D. & McDonald S.S. 2002. Micronutrients in cancer chemoprevention. Cancer Metast. Rev. 21: 217–230. http://dx.doi.org/10.1023/A:1021202709003CrossrefGoogle Scholar

  • [10] Habig W.H. & Jakoby W.B. 1974. Assay for differentiation of glutathione S-transferases. Methods Enzymol. 77: 398–405. http://dx.doi.org/10.1016/S0076-6879(81)77053-8CrossrefGoogle Scholar

  • [11] Halliwel B. 1994. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence. Lancet 344: 721–724. http://dx.doi.org/10.1016/S0140-6736(94)92211-XCrossrefGoogle Scholar

  • [12] Handelman G.J., Packer L. & Cross C.E. 1996. Destruction of tocopherols, carotenoids and retinal in human plasma by cigarette smoke. Am. J. Clin. Nutr. 63: 559–565. Google Scholar

  • [13] Hilbert J. & Mohsenin V. 1996. Adaptation of lung antioxidants to cigarette smoking in humans. Chest 110: 916–920. Google Scholar

  • [14] Hochman A., Liang H., Offen D., Melamed E. & Sternin H. 2000. Developmental changes in antioxidant enzymes and oxidative damage in kidneys, liver and brain of bcl-2 knockout mice. Cell. Mol. Biol. 46: 41–52. Google Scholar

  • [15] Ip C., Dong Y. & Ganther H.E. 2002. New concepts in selenium chemoprevention. Cancer Metast. Rev. 21: 281–289. http://dx.doi.org/10.1023/A:1021263027659CrossrefGoogle Scholar

  • [16] Lowry O.H., Rosebrough N.J., Faar A.L. & Randall R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275. Google Scholar

  • [17] Luck H. 1963. Catalase, pp. 885–894. In: Bergmeyer H.U. (ed.), Methods of Enzymatic Analyses, Verlag Chemie, Academic Press, Weinheim, New York. Google Scholar

  • [18] McCord J.M. & Fridovich I.J. 1969. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244: 6049–6055. Google Scholar

  • [19] Muzakova V., Kandar R., Vojtisek P., Skalicky J., Vankova R., Cegan A. & Cervincova Z. 2001. Antioxidant vitamin levels and glutathione peroxidase activity during ischemia/reperfusion in myocardial infarction. Physiol. Res. 50: 389–396. Google Scholar

  • [20] Ozkan A. & Fiskin K. 2004. Free radicals, carcinogenesis and antioxidant enzymes. Tr. J. Hematol. Oncol. 14: 52–60. Google Scholar

  • [21] Phillips D.H. 2002. Smoking-related DNA and protein adducts in human tissue. Carcinogenesis 23: 1979–2004. http://dx.doi.org/10.1093/carcin/23.12.1979CrossrefGoogle Scholar

  • [22] Pryor W.A. & Stone K. 1993. Oxidants in cigarette smoke: radicals, hydrogen peroxide, peroxynitrate, and peroxynitrite. Ann. New York Acad. Sci. 686: 12–27. http://dx.doi.org/10.1111/j.1749-6632.1993.tb39148.xCrossrefGoogle Scholar

  • [23] Rani P.J.A. & Panneerselvam C. 2001. Carnitine as a free radical scavenger in aging. Exp. Geront. 36: 1713–1726. http://dx.doi.org/10.1016/S0531-5565(01)00116-4CrossrefGoogle Scholar

  • [24] Rohini G., Sabitha K.E. & Devi C.S.S. 2004. Bacopa monniera Linn. extract modulates antioxidant and marker enzyme status in fibrosarcoma bearing rats. Ind. J. Exp. Biol. 42: 776–780. Google Scholar

  • [25] Sumathy T., Subramanian S., Govindasamy S., Balakrishna K. & Veluchamy G. 2001. Protective role of Bacopa monniera on morphine induced hepatotoxicity in rats. Phytotherapy Res. 15: 643–645. http://dx.doi.org/10.1002/ptr.1007CrossrefGoogle Scholar

About the article

Published Online: 2007-06-01

Published in Print: 2007-06-01


Citation Information: Biologia, Volume 62, Issue 3, Pages 360–364, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-007-0060-1.

Export Citation

© 2007 Institute of Molecular Biology, Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
[2]
Kumar Ganesan, Muthukumaran Jayachandran, and Baojun Xu
Critical Reviews in Food Science and Nutrition, 2018, Volume 58, Number 7, Page 1165
[3]
Sha Li, Hor-Yue Tan, Ning Wang, Zhang-Jin Zhang, Lixing Lao, Chi-Woon Wong, and Yibin Feng
International Journal of Molecular Sciences, 2015, Volume 16, Number 12, Page 26087
[4]
Irena Musik, Anna Hordyjewska, Anna Boguszewska-Czubara, and Kazimierz Pasternak
Pharmacological Reports, 2009, Volume 61, Number 5, Page 885
[5]
Irena Musik, Małgorzata Kiełczykowska, and Joanna Kocot
BioMetals, 2013, Volume 26, Number 5, Page 763
[6]
Srikanta Ghosh, Shashi Shankar Tiwari, Sharad Srivastava, Anil Kumar Sharma, Sachin Kumar, D.D. Ray, and A.K.S. Rawat
Veterinary Parasitology, 2013, Volume 192, Number 1-3, Page 259
[7]
P. Reckziegel, N. Boufleur, R.C.S. Barcelos, D.M. Benvegnú, C.S. Pase, L.G. Muller, A.M. Teixeira, R. Zanella, A.C.P. Prado, R. Fett, J.M. Block, and M.E. Burger
Ecotoxicology and Environmental Safety, 2011, Volume 74, Number 6, Page 1770

Comments (0)

Please log in or register to comment.
Log in