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Volume 62, Issue 4


New larval agar-based diet for laboratory rearing of Mediterranean fruit fly Ceratitis capitata (Diptera, Tephritidae)

Martina Pašková
Published Online: 2007-08-01 | DOI: https://doi.org/10.2478/s11756-007-0091-7


Five larval diets for laboratory rearing of Ceratitis capitata were tested. These diets were based on wheat bran, microcellulose, potato starch and agar. To evaluate the quality of diet, pupal rearing efficacy and pupal weight were checked. The best results were obtained with an agar based diet used for Manduca sexta laboratory rearing. To simplify the preparation and to reduce the cost of this diet, a new formulation was developed. Larvae reared on the new agar-based diet achieved higher pupal rearing efficacy than larvae reared on bran diet recently used in medfly mass rearing facilities. Heat treated medflies reared on the new agar-based diet achieved similar pupal rearing efficacy with heat treated medflies reared on bran diet. When testing population density, higher pupal rearing efficacy was again achieved on new diet. The highest pupal rearing efficacy was achieved with 100 eggs per 25 g of diet, lowest with 500 eggs per 25 g of diet. Concerning pupal weight, there was no difference in results achieved on Petri dishes with different larval population densities. Larvae reared on new agar-based diet reached better results than larvae reared on bran diet. The preparation of the new diet is simple and the cost is low, so it is good for laboratory tests and rearing.

Keywords: Ceratitis capitata; larval diet; laboratory rearing; bran; agar

  • [1] Bell R.A. & Joachim F.A. 1976. Techniques for rearing laboratory colonies of tobacco hornworms and pink bollworms. Ann. Entomol. Soc. Am. 69: 365–373. CrossrefGoogle Scholar

  • [2] Caceres C. 2002. Mass rearing of temperature sensitive genetic sexing strains in the Mediterranean fruit fly (Ceratitis capitata). Genetica 116: 107–116. http://dx.doi.org/10.1023/A:1020967810703CrossrefGoogle Scholar

  • [3] Chan H.T. Jr., Ako H., Niino-Duponte R.Y., Carpenter J.R. & Jang E.B. 2000. Composition of Mediterranean fruit fly third instar larvae (Diptera: Tephritidae) and diet: nutrient balance studies on amino acids, minerals and nutrient composition in fresh and spent mass rearing diets, pp. 567–576. In: Tan K.H. (ed.), Area-Wide Management of Fruit Flies and Other Major Insect Pests, Universiti Sains Malaysia Press, Penang, Malaysia. Google Scholar

  • [4] Chang C.L., Kurashima R. & Albrecht C. 2000. Effect of limiting concentrations of growth factors in mass rearing diets for Ceratitis capitata larvae (Diptera: Tephritidae). Ann. Entomol. Soc. Am. 93: 898–903. http://dx.doi.org/10.1603/0013-8746(2000)093[0898:EOLCOG]2.0.CO;2CrossrefGoogle Scholar

  • [5] Chang C.L., Kurashima R. & Albrecht C.P. 2001. Larval development of Ceratitis capitata (Diptera: Tephritidae) on a meridic diet. Ann. Entomol. Soc. Am. 94: 433–437. http://dx.doi.org/10.1603/0013-8746(2001)094[0433:LDOCCA]2.0.CO;2CrossrefGoogle Scholar

  • [6] Dukas R., Prokopy R.J. & Duan J.J. 2001. Effects of larval competition on survival and growth in Mediterranean fruit flies. Ecol. Entomol. 26: 587–593. http://dx.doi.org/10.1046/j.1365-2311.2001.00359.xCrossrefGoogle Scholar

  • [7] Eymann M. & Friend W.G. 1985. Effects of vitamin supplements on the development of onion maggots (Diptera: Anthomyiidae) on axenic onion agar. Can. Entomol. 117: 475–479. http://dx.doi.org/10.4039/Ent117475-4CrossrefGoogle Scholar

  • [8] Fay H.A.C. & Wornoayporn V. 2002. Inert reusable substrates as potential replacements for wheat brain in larval diets for Mediterranean fruit fly, Ceratitis capitata (Wied.) (Diptera: Tephritidae). J. Appl. Entomol. 126: 92–96. http://dx.doi.org/10.1046/j.1439-0418.2002.00624.xCrossrefGoogle Scholar

  • [9] Hooper G.H.S. 1987. Application of quality control procedures to large scale rearing of the Mediterranean fruit fly. Entomol. Exp. Appl. 44: 161–167. http://dx.doi.org/10.1007/BF00367625CrossrefGoogle Scholar

  • [10] IAEA/FAO/USDA 1998. A Manual of Quality Control for Fruit Flies. Version 4.0. International Atomic Energy Agency, Vienna, Austria. Google Scholar

  • [11] Kumar N.P. & Anad M. 1992. Effects of vitamins on the growth and survival of Dacus dorsalis (Hendel) maggots. Indian J. Entomol. 54: 139–149. Google Scholar

  • [12] Lindquist D.A. 2000. Pest management strategies: area-wide and conventional, pp. 13–19. In: Tan K.H. (ed.), Area-Wide Management of Fruit Flies and Other Major Insect Pests, Universiti Sains Malaysia Press, Penang, Malaysia. Google Scholar

  • [13] Mitchell S., Tanaka N. & Steiner L.F. 1965. Methods of mass culturing melon flies and oriental and Mediterranean fruit flies. USDA ARS, pp. 33–104. Google Scholar

  • [14] Tanaka N., Steiner L.F., Ohinata K. & Okamoto R. 1969. Low cost larval rearing medium for mass production of Oriental and Mediterranean fruit flies. J. Econ. Entomol. 62: 967–968. CrossrefGoogle Scholar

  • [15] Vargas R.I., Mitchell S., Hsu C.L. & Walsh W.A. 1994. Laboratory evaluation of diets of processed corncob, torula yeast, and wheat germ on four developmental stages of Mediterranean fruit fly (Diptera: Tephritidae). J. Econ. Entomol. 87: 91–95. Google Scholar

About the article

Published Online: 2007-08-01

Published in Print: 2007-08-01

Citation Information: Biologia, Volume 62, Issue 4, Pages 477–481, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-007-0091-7.

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© 2007 Institute of Zoology, Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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