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Biologia




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Volume 63, Issue 2

Issues

Genetic variability within isolates of Colletotrichum lindemuthianum belonging to race 65 from the state of Minas Gerais, Brazil

Francine Ishikawa
  • Laboratório de Resistęncia de Plantas a Doenças, Departamento de Biologia, Universidade Federal de Lavras, Caixa Postal 3037, 37200-000, Lavras, Minas Gerais, Brazil
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/ Elaine Souza
  • Laboratório de Resistęncia de Plantas a Doenças, Departamento de Biologia, Universidade Federal de Lavras, Caixa Postal 3037, 37200-000, Lavras, Minas Gerais, Brazil
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/ Livia Davide
  • Laboratório de Resistęncia de Plantas a Doenças, Departamento de Biologia, Universidade Federal de Lavras, Caixa Postal 3037, 37200-000, Lavras, Minas Gerais, Brazil
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Published Online: 2008-03-27 | DOI: https://doi.org/10.2478/s11756-008-0039-6

Abstract

Colletotrichum lindemuthianum, the causal agent of anthracnose in the common bean (Phaseolus vulgaris), presents a wide genetic and pathogenic variability that gives rise to complications in the development of resistant bean cultivars. The aim of this study was to identify the variability within race 65 of C. lindemuthianum, the race most commonly encountered in Brazil, through randomly amplified polymorphic DNA (RAPD) and anastomosis analyses. Thirteen isolates of race 65, collected in different years and from various host cultivars located in diverse areas of the state of Minas Gerais, Brazil, were investigated. Twenty-four RAPD primers were employed and 83 polymorphic bands amplified. Genetic similarities were estimated from the Sorensen-Dice coefficient and ranged from 0.54 to 0.82. The dendrogram obtained by cluster analysis classified the isolates into 11 separate groups. For the purposes of the analysis of anastomosis, isolates were considered to be compatible when the fusion of hyphae from different isolates could be observed. The proportion of compatible reactions for each isolate was estimated and similarity estimates, based on the Russel & Rao coefficient, ranged from 0.28 to 0.85. Isolates were classified into 11 anastomosis groups, 10 of which were formed by only one isolate. Although isolates LV61, LV73 and LV58 were classified in the same anastomosis group, they were genetically distinct according to RAPD analysis. Results from both RAPD and anastomosis analyses revealed great variability within C. lindemuthianum race 65.

Keywords: Colletotrichum lindemuthianum; Phaseolus vulgaris; common bean; anthracnose; genetic similarity; molecular markers; anastomosis groups

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About the article

Published Online: 2008-03-27

Published in Print: 2008-04-01


Citation Information: Biologia, Volume 63, Issue 2, Pages 156–161, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-008-0039-6.

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© 2008 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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