[1] Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W. & Lipman D.J. 1997. Gapped BLAST and PSIBLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402. http://dx.doi.org/10.1093/nar/25.17.3389CrossrefGoogle Scholar

[2] Benson D.A., Karsch-Mizrachi I., Lipman D.J., Ostell J. & Wheeler D.L. 2007. GenBank. Nucleic Acids Res. 35 (Database issue): D21–D25. http://dx.doi.org/10.1093/nar/gkl986CrossrefGoogle Scholar

[3] Gasteiger E., Gattiker A., Hoogland C., Ivanyi I., Appel R.D. & Bairoch A. 2003. ExPASy: the proteomics server for indepth protein knowledge and analysis. Nucleic Acids Res 31: 3784–3788. Harmon A.C., Gribskov M. & Harper J.F. 2000. CDPKs — a kinase for every Ca2+ signal? Trends Plant Sci. 5: 154–159. http://dx.doi.org/10.1093/nar/gkg563CrossrefGoogle Scholar

[4] Harper J.F., Bimder B.M. & Sussman M.R. 1993. Calcium and lipid regulation of an A. thaliana protein kinase expressed in Escherichia coli. Biochemistry 32: 3282–3290. http://dx.doi.org/10.1021/bi00064a010CrossrefGoogle Scholar

[5] Harper J.F., Sussman M.R., Schaller G.E., Putnam-Evans C., Charbonneau H. & Harman A.C. 1991. A calcium-dependent protein kinase with a regulatory domain similar to calmodulin. Science 252: 951–954. http://dx.doi.org/10.1126/science.1852075CrossrefGoogle Scholar

[6] Hrabak E.M., Chan C.W., Gribskov M., Harper J.F., Choi J.H., Halford N., Kudla J., Luan S., Nimmo H.G., Sussman M.R., Thomas M., Walker-Simmons K., Zhu J.K., Harmon A.C. 2003. The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol. 132: 666–680. http://dx.doi.org/10.1104/pp.102.011999CrossrefGoogle Scholar

[7] Jaakola L., Pirtila A.M., Halonen M. & Hohtola A. 2001. Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit. Mol. Biotechnol. 19: 201–203. http://dx.doi.org/10.1385/MB:19:2:201CrossrefGoogle Scholar

[8] Kashkush K., Feldman M. & Levy A.A. 2003. Transcriptional activation of retrotransposons alters the expression of adjacent genes in wheat. Nat. Genet. 33: 102–106. http://dx.doi.org/10.1038/ng1063CrossrefGoogle Scholar

[9] Kumar S., Tamura K. & Nei M. 2004. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform. 5: 150–163. http://dx.doi.org/10.1093/bib/5.2.150CrossrefGoogle Scholar

[10] Lee H.S. & Chen Z.J. 2001. Protein-coding genes are epigenetically regulated in Arabidopsis polyploids. Proc. Natl. Acad. Sci. USA 98: 6753–6758. http://dx.doi.org/10.1073/pnas.121064698CrossrefGoogle Scholar

[11] Llop-Tous I., Dominguez-Puigjaner E. & Vendrell M. 2002. Characterization of a strawberry cDNA clone homologous to calcium-dependent protein kinases that is expressed during fruit ripening and affected by low temperature. J. Exp. Bot. 53: 2283–2285. http://dx.doi.org/10.1093/jxb/erf103CrossrefGoogle Scholar

[12] Lu B.B., Pan X.Z., Zhang L., Huang B.B., Sun L.N., Li B., Yi B., Zheng S.Q., Yu X.J., Ding R.X. & Chen W.S. 2006. Genes responsive to autopolyploidy in Isatis indigotica using Arabidopsis thaliana affymetrix genechips. Plant Mol. Biol. Rep. 24: 197–204. http://dx.doi.org/10.1007/BF02914058CrossrefGoogle Scholar

[13] Qiao C.Z. & Li H. 1994. Cultivation and popularization for tetraploidy strain of Isatis indigotica. Chinese Traditional Herb 17: 3–6. Google Scholar

[14] Qiao C.Z., Wu M.S., Dai F.B., Cui X. & Li L. 1989. Studies on polyploid breeding of Isatis indigotica Fort. Acta Botanica Sinica 31: 678–683. Google Scholar

[15] Romeis T., Ludwig A., Martin R. & Jones J. 2001. Calciumdependent protein kinases play an essential role in a plant defence response. EMBO J. 20: 5556–5567. http://dx.doi.org/10.1093/emboj/20.20.5556CrossrefGoogle Scholar

[16] Rudd J.J. & Franklin-Tong V.E. 2001. Unravelling response-specificity in Ca2+ signaling pathways in plant cells. New Phytol. 151: 7–33. http://dx.doi.org/10.1046/j.1469-8137.2001.00173.xCrossrefGoogle Scholar

[17] Saijo Y., Hata S., Sheen J. & Izui K. 1997. cDNA cloning and prokaryotic expression of maize calcium-dependent protein kinases. Biochim. Biophys. Acta 1350: 109–114. Google Scholar

[18] Schranz M.E. & Osborn T.C. 2000. Novel flowering time and variation in the resynthesized polyploid Brassica napus. J. Heredity 91: 242–246. http://dx.doi.org/10.1093/jhered/91.3.242CrossrefGoogle Scholar

[19] Siow Y.L., Gong Y., Au-Yeung K.K., Woo C.W. & Choy P.C. 2005. Emerging issues in traditional Chinese medicine. Can. J. Physiol. Pharmacol. 82: 321–334. http://dx.doi.org/10.1139/y05-029CrossrefGoogle Scholar

[20] The Arabidopsis Initiative 2000. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796–815. http://dx.doi.org/10.1038/35048692CrossrefGoogle Scholar

[21] Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. & Higgins D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24: 4876–4882. http://dx.doi.org/10.1093/nar/25.24.4876CrossrefGoogle Scholar

[22] Urao T., Katagiri T., Mizoguchi T., Yamaguchi-Shinozaki K., Hayashida N. & Shinozaki K. 1994. An Arabidopsis thaliana cDNA encoding Ca2+-dependent protein kinase. Plant Physiol. 105: 1461–1462. http://dx.doi.org/10.1104/pp.105.4.1461Google Scholar

[23] Wang Y., Qiao C.Z., Liu S. & Hang H.M. 2000. Evaluation on antiendotoxic action and antiviral action in vitro of tetraploid Isatis indigotica. Chinese Traditional Herb 25: 327–329. Google Scholar

[24] Wu Y.C., Hsu J.H., Liu I.M., Liou S.S., Su H.C. & Cheng J.T. 2002. Increase of insulin sensitivity in diabetic rats received Die-Huang-Wan, a herbal mixture used in Chinese traditional medicine. Acta Pharmacologica Sinica 23: 1181–1187. Google Scholar

[25] Xie C., Kokubun T., Houghton P.J. & Simmonds M.S. 2004. Antibacterial activity of the Chinese traditional medicine, Zi Hua Di Ding. Phytother. Res. 18: 497–500. http://dx.doi.org/10.1002/ptr.1497CrossrefGoogle Scholar

[26] Yoon G.M., Cho H.S., Ha H.J., Liu J.R. & Lee H.S.P. 1999. Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein. Plant Mol. Biol. 39: 991–1001. http://dx.doi.org/10.1023/A:1006170512542CrossrefGoogle Scholar

[27] Zhang B., Wang Z.F., Tang M.Z. & Shi Y.L. 2005. Growth inhibition and apoptosis-induced effect on human cancer cells of toosendanin, a triterpenoid derivative from Chinese traditional medicine. Invest. New Drugs 23: 547–553. http://dx.doi.org/10.1007/s10637-005-0909-5CrossrefGoogle Scholar