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Volume 63, Issue 6


Comparative study of chloroplast morphology and ontogeny in Asterochloris (Trebouxiophyceae, Chlorophyta)

Pavel Škaloud
  • Faculty of Science, Department of Botany, Charles University in Prague, Benátská 2, CZ-12801, Praha 2, Czech Republic
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/ Ondřej Peksa
  • Faculty of Science, Department of Botany, Charles University in Prague, Benátská 2, CZ-12801, Praha 2, Czech Republic
  • The Westbohemian Museum in Pilsen, Kopeckého sady 2, CZ-30100, Plzeň, Czech Republic
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Published Online: 2008-12-04 | DOI: https://doi.org/10.2478/s11756-008-0115-y


Confocal laser scanning microscopy was utilized to compare the chloroplast morphology and ontogeny among five strains of the green alga Asterochloris. Parsimony analysis inferred from the rDNA ITS sequences confirmed their placement in three distinct lineages: Asterochloris phycobiontica, Trebouxia pyriformis and Asterochloris sp. Examination by confocal microscopy revealed the existence of interspecific differences in the chloroplast ontogeny of Asterochloris; this was based upon either specific chloroplast structures observed in a single species, or on the differential timing of particular ontogenetic sequences. The occurrence of flat parietal chloroplasts prior to cell division, considered as a basic morphological discriminative character of Asterochloris, was clearly associated with the process of aplanosporogenesis. By contrast, chloroplast transformation prior to the formation of autospores proceeded simply by the multiple fission of the chloroplast matrix in the cell lumen.

Keywords: Asterochloris; Trebouxia; chloroplast morphology; confocal microscopy; ITS; molecular phylogeny

  • [1] Abramoff M.D., Magelhaes P.J. & Ram S.J. 2004. Image processing with Image J. Biophoton. Int. 11: 36–42. Google Scholar

  • [2] Ahmadjian V. 1960. Some new and interresting species of Trebouxia, a genus of lichenized algae. Am. J. Bot. 47: 677–683. http://dx.doi.org/10.2307/2439519CrossrefGoogle Scholar

  • [3] Ahmadjian V. 1993. The lichen symbiosis. John Wiley & Sons, New York, NY, USA, 250 pp. Google Scholar

  • [4] Beck A., Friedl T. & Rambold G. 1998. Selectivity of photobiont choice in a defined lichen community: inferences from cultural and molecular studies. New Phytol. 139: 709–720. http://dx.doi.org/10.1046/j.1469-8137.1998.00231.xCrossrefGoogle Scholar

  • [5] Bischoff H.W. & Bold H.C. 1963. Phycological Studies. IV. Some soil algae from enchanted rock and related algal species. Univ. Texas Publ. 6318: 1–95. Google Scholar

  • [6] DePriest P.T. 2004. Early molecular investigations of lichenforming symbionts: 1986–2001. Annu. Rev. Microbiol. 58: 273–301. http://dx.doi.org/10.1146/annurev.micro.58.030603.123730CrossrefGoogle Scholar

  • [7] Doyle J.J. & Doyle J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15. Google Scholar

  • [8] Friedl T. 1995. Inferring taxonomic positions and testing genus level assignments in coccoid green lichen algae: A phylogenetic analysis of 18S ribosomal RNA sequences from Dictyochloropsis reticulata and from members of the genus Myrmecia (Chlorophyta, Trebouxiophyceae Cl. Nov.). J. Phycol. 31: 632–639. http://dx.doi.org/10.1111/j.1529-8817.1995.tb02559.xCrossrefGoogle Scholar

  • [9] Friedl T. & Büdel B. 1996. Photobionts, pp. 8–23. In: Nash T.H. (ed.), Lichen biology. Cambridge University Press, Cambridge, UK. Google Scholar

  • [10] Friedl T. & Gärtner G. 1988. Trebouxia (Pleurastrales, Chlorophyta) as a phycobiont in the lichen genus Diploschistes. Arch. Protistenkd. 135: 147–158. Google Scholar

  • [11] Friedl T. & Rokitta C. 1997. Species relationships in the lichen alga Trebouxia (Chlorophyta, Trebouxiophyceae): Molecular phylogenetic analyses of nuclear-encoded large subunit rRNA gene sequences. Symbiosis 23: 125–148. Google Scholar

  • [12] Friedl T. & Zeltner C.T. 1994. Assessing the relationships of some coccoid green lichen algae and the Microthamniales (Chlorophyta) with 18S ribosomal RNA gene sequence comparisons. J. Phycol. 30: 500–506. http://dx.doi.org/10.1111/j.0022-3646.1994.00500.xCrossrefGoogle Scholar

  • [13] Gärtner G. 1985. Die Gattung Trebouxia Puymaly (Chlorellales, Chlorophyceae). Arch. Hydrobiol. Suppl., Algol. Stud. 41: 495–548. Google Scholar

  • [14] Hesse M. 2001. Zum Gedenken an Frau emer. O. Prof. Dr. Elisabeth Woess. Verh. Zool.-Bot. Ges. Österreich 138: 275–278. Google Scholar

  • [15] Hildreth K.C. & Ahmadjian V. 1981. A study of Trebouxia and Pseudotrebouxia isolates from different lichens. Lichenologist 13: 65–86. http://dx.doi.org/10.1017/S0024282981000078CrossrefGoogle Scholar

  • [16] Kroken S. & Taylor J.W. 2000. Phylogenetic species, reproductive mode, and specifity of the green alga Trebouxia forming lichens with the fungal genus Letharia. Bryologist 103: 645–660. http://dx.doi.org/10.1639/0007-2745(2000)103[0645:PSRMAS]2.0.CO;2CrossrefGoogle Scholar

  • [17] Kumar S., Tamura K. & Nei M. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief. Bioinform. 5: 150–163. http://dx.doi.org/10.1093/bib/5.2.150CrossrefGoogle Scholar

  • [18] Neustupa J., Eliáš M. & Šejnohová L. 2007. A taxonomic study of two Stichococcus species (Trebouxiophyceae, Chlorophyta) with a starch-enveloped pyrenoid. Nova Hedwigia 84: 51–63. http://dx.doi.org/10.1127/0029-5035/2007/0084-0051CrossrefWeb of ScienceGoogle Scholar

  • [19] Piercey-Normore M.D. & DePriest P.T. 2001. Algal switching among lichen symbioses. Am. J. Bot. 88: 1490–1498. http://dx.doi.org/10.2307/3558457CrossrefGoogle Scholar

  • [20] Rambold G., Friedl T. & Beck A. 1998. Photobionts in lichens: Possible indicators of phylogenetic relationships? Bryologist 101: 392–397. CrossrefGoogle Scholar

  • [21] Schwendener S. 1867. Über die wahre Natur der Flechtengonidien. Verh. Schweiz. Naturforsch. Ges. 9–11. Google Scholar

  • [22] Škaloud P., Neustupa J., Radochová B. & Kubínová L. 2005. Confocal microscopy of chloroplast morphology and ontogeny in three strains of Dictyochloropsis (Trebouxiophyceae, Chlorophyta). Phycologia 44: 261–269. http://dx.doi.org/10.2216/0031-8884(2005)44[261:CMOCMA]2.0.CO;2CrossrefGoogle Scholar

  • [23] Swofford D.L. 2003. PAUP*. Phylogenetic analysis using parsimony (and other methods). Version 4. Sinauer Associates, Sunderland, Massachusetts. Google Scholar

  • [24] Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. & Higgins D.G. 1997. The ClustalX Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acid. Res. 25: 4876–4882. http://dx.doi.org/10.1093/nar/25.24.4876CrossrefGoogle Scholar

  • [25] Tschermak-Woess E. 1980. Asterochloris phycobiontica, gen. at spec., nov., der Phycobiont der Flechte Varicellaria carneonivea. Pl. Syst. Evol. 135: 279–294. http://dx.doi.org/10.1007/BF00983192CrossrefGoogle Scholar

  • [26] Tschermak-Woess E. 1988. The algal partner, pp. 39–92. In: Galun M. (ed.), CRC Handbook of Lichenology, Vol. 1., CRC-Press, Boca Raton, Florida. Google Scholar

  • [27] Tschermak-Woess E. 1989. Developmental studies in trebouxioid algae and taxonomical consequences. Pl. Syst. Evol. 164: 161–195. http://dx.doi.org/10.1007/BF00940436CrossrefGoogle Scholar

  • [28] White T.J., Bruns T., Lee S. & Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 345–322. In: Innis M.A., Gelfand D.H., Sninsky J.J. & White T.J. (eds), PCR Protocols: A Guide to Methods and Applications, Academic Press, San Diego. Google Scholar

About the article

Published Online: 2008-12-04

Published in Print: 2008-12-01

Citation Information: Biologia, Volume 63, Issue 6, Pages 873–880, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-008-0115-y.

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© 2008 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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