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Volume 63, Issue 6


Domain evolution in the GH13 pullulanase subfamily with focus on the carbohydrate-binding module family 48

Martin Machovič / Štefan Janeček
  • Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, SK-84551, Bratislava, Slovakia
  • Department of Biotechnologies, Faculty of Natural Sciences, University of SS. Cyril and Methodius, Nám. J. Herdu 2, SK-91701, Trnava, Slovakia
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Published Online: 2008-12-04 | DOI: https://doi.org/10.2478/s11756-008-0162-4


Glycoside hydrolase (GH) family 13 comprises about 30 different specificities. Four of them have been proposed to form the GH13 pullulanase subfamily: pullulanase, isoamylase, maltooligosyl trehalohydrolase and branching enzyme forming the seven CAZy GH13 subfamilies: GH13 8-GH13 14. Recently, a new family of carbohydrate-binding modules (CBMs), the family CBM48 has been established containing the putative starch-binding domains from the pullulanase subfamily, the β-subunit of AMP-activated protein kinase and some other GH13 enzymes with pullulanase and/or α-amylase-pullulanase specificity. Since all of these enzymes are multidomain proteins and the structure for at least one representative of each enzyme specificity has already been determined, the main goal of the present study was to elucidate domain evolution within this GH13 pullulanase subfamily (84 real enzymes) focusing on the CBM48 module. With regard to CBM48 positioning in the amino acid sequence, the N-terminal end of a protein appears to be a predominant position. This is especially true for isoamylases and maltooligosyl trehalohydrolases. Secondary structure-based alignment of CBM modules from CBM48, CBM20 and CBM21 revealed that several residues known as consensus for CBM20 and CBM21 could also be identified in CBM48, but only branching enzymes possess the aromatic residues that correspond with the two tryptophans forming the evolutionary conserved starch-binding site 1 in CBM20. The evolutionary trees constructed for the individual domains, complete alignment, and the conserved sequence regions of the α-amylase family were found to be comparable to each other (except for the C-domain tree) with two basic parts: (i) branching enzymes and maltooligosyl trehalohydrolases; and (ii) pullulanases and isoamylases. Taxonomy was respected only within clusters with pure specificity, i.e. the evolution of CBM48 reflects the evolution of specificities rather than evolution of species. This is a feature different from the one observed for the starch-binding domain of the family CBM20 where the starch-binding domain evolution reflects the evolution of species.

Keywords: α-amylase enzyme family; pullulanase subfamily; starch-binding domain; domain evolution; evolutionary tree

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Published Online: 2008-12-04

Published in Print: 2008-12-01

Citation Information: Biologia, Volume 63, Issue 6, Pages 1057–1068, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-008-0162-4.

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Biopolymers, 2015, Volume 103, Number 4, Page 203
Il-Nam Oh, Jay-lin Jane, Kan Wang, Jong-Tae Park, and Kwan-Hwa Park
Extremophiles, 2015, Volume 19, Number 2, Page 363
Marek Gabriško and Štefan Janeček
Journal of Molecular Evolution, 2011, Volume 72, Number 1, Page 104
Fu-Pang Lin, Yi-Hsuan Ho, Hsu-Yang Lin, and Hui-Ju Lin
Extremophiles, 2012, Volume 16, Number 3, Page 395
Catherine Eyre, Wafa Muftah, Jennifer Hiscox, Julie Hunt, Peter Kille, Lynne Boddy, and Hilary J. Rogers
Fungal Biology, 2010, Volume 114, Number 8, Page 646
Camilla Christiansen, Maher Abou Hachem, Štefan Janeček, Anders Viksø-Nielsen, Andreas Blennow, and Birte Svensson
FEBS Journal, 2009, Volume 276, Number 18, Page 5006
Fu-Pang Lin, Hsiu-Yen Ma, Hui-Ju Lin, Shiu-Mei Liu, and Wen-Shyong Tzou
Applied Biochemistry and Biotechnology, 2011, Volume 165, Number 3-4, Page 1047
Štefan Janeček, Birte Svensson, and E. Ann MacGregor
Enzyme and Microbial Technology, 2011, Volume 49, Number 5, Page 429
Malene Bech Vester-Christensen, Maher Abou Hachem, Birte Svensson, and Anette Henriksen
Journal of Molecular Biology, 2010, Volume 403, Number 5, Page 739
Eun-Seong Seo, Camilla Christiansen, Maher Abou Hachem, Morten Nielsen, Kenji Fukuda, Sophie Bozonnet, Andreas Blennow, Nushin Aghajari, Richard Haser, and Birte Svensson
Biologia, 2008, Volume 63, Number 6

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