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Biologia




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Volume 64, Issue 4

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Expression of antiapoptotic protein survivin in malignant melanoma

Marian Adamkov
  • Institute of Histology and Embryology, Jessenius Faculty of Medicine, Comenius University, Malá Hora 4, SK-03601, Martin, Slovakia
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/ L’udovít Lauko / Július Rajčáni / Soňa Bálentová
  • Institute of Histology and Embryology, Jessenius Faculty of Medicine, Comenius University, Malá Hora 4, SK-03601, Martin, Slovakia
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/ Silvia Rybárová
  • Department of Anatomy, Faculty of Medicine, P. J. Šafárik University, Šrobárova 2, SK-04180, Košice, Slovakia
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/ Dušan Mištuna
  • Clinic of Surgery, Jessenius Faculty of Medicine, Comenius University, MFN, Kollárova 2, SK-03601, Martin, Slovakia
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/ Dagmar Statelová
  • Clinic of Stomatology and Maxillofacial Surgery, Jessenius Faculty of Medicine, Comenius University, MFN, Kollárova 2, SK-03601, Martin, Slovakia
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Published Online: 2009-07-17 | DOI: https://doi.org/10.2478/s11756-009-0134-3

Abstract

We examined the expression of potential tumor marker survivin by immunohistochemical staining using antisurvivin antibody (DAKO, Clone 12C4) in a panel of 25 malignant melanomas. In each section, we assessed the percentage of positively stained tumor cells, the intensity of staining and its subcellular localization. Survivin was present in 23 out of 25 cases (92%). Nuclear staining was found in 2 of these 23 cases (8.7%) only, while cytoplasmic staining only was seen in 3 of them (13%). The combined nuclear as well as cytoplasmic localization of survivin was demonstrated in 18 out of 23 cases (78.3%). In 2 cases revealing nuclear staining only, the worse histological features were more pronounced than in 3 cases with cytoplasmic staining only. Our results suggest that nuclear positivity of survivin may correlate with the degree of malignancy. In addition, we conclude that overexpression of survivin involved in the pathogenesis of melanoma represents an important diagnostic marker.

Keywords: melanoma; survivin; immunohistochemical staining; diagnostic marker

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About the article

Published Online: 2009-07-17

Published in Print: 2009-08-01


Citation Information: Biologia, Volume 64, Issue 4, Pages 840–844, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-009-0134-3.

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© 2009 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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