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Biologia

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Volume 64, Issue 5

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Molecular identification of mycorrhizal fungi in Dactylorhiza sambucina (Orchidaceae)

Giuseppe Pellegrino / Francesca Bellusci
Published Online: 2009-09-10 | DOI: https://doi.org/10.2478/s11756-009-0175-7

Abstract

We amplified and sequenced internal transcribed spacer (ITS) region of the nuclear ribosomal repeat from fungi in roots of Dactylorhiza sambucina (Orchidaceae) and used the extended database to identify the mycorrhizal fungi. We molecularly identified three ITS recovered from D. sambucina roots, one belonging to Rhizoctonia group, and two to ascomycetes, for the first time in Orchidoidae. In many cases, two sequence types were found from the same orchid root, providing that two taxa may be involved in mycorrhizal formation (multiple mycobiont colonization). Moreover, we demonstrated that D. sambucina plants, irrespective of their colour polymorphism, possess roots containing several fungi belonging to both asco- and basidiomycetes.

Keywords: Dactylorhiza sambucina; ITS; mycorrhizal fungi; Orchidaceae

  • [1] Bidartondo M.I., Burghardt B., Gebauer G., Bruns T.D. & Read D.J. 2004. Changing partners in the dark: isotopic and molecular evidence of ectomycorrhizal liaisons between forest orchids and trees. Proc. R. Soc. London Series B-Biol. 271: 1799–1806. http://dx.doi.org/10.1098/rspb.2004.2807CrossrefGoogle Scholar

  • [2] Clements M.A. 1988. Orchid mycorrhizal associations. Lindleyana 3: 73–86. Google Scholar

  • [3] Currah R.S., Zettler L.W. & McInnis T.M. 1997. Epulorhiza inquilina sp. nov. from Platanthera (Orchidaceae) and a key to Epulorhiza species. Mycotaxon 61: 335–342. Google Scholar

  • [4] Curtis J.T. 1939. The relation of specificity of orchid mycorrhizal fungi to the problem of symbiosis. Am. J. Bot. 26: 390–399. http://dx.doi.org/10.2307/2436839CrossrefGoogle Scholar

  • [5] Doyle J.J. 1991. DNA protocols for plants — CTAB total DNA isolation, pp. 283–293. In: Hewitt G.M. & Johnston A. (eds), Molecular techniques in taxonomy. Springer Verlag, Berlin, Germany. Google Scholar

  • [6] Dressler R.L. 1981. The orchids: natural history and classification. Harvard University Press, Cambridge, MA, USA. Google Scholar

  • [7] Gardes M. & Bruns T.D. 1993. ITS primers with enhanced specificity for Basidiomycetes-application to the identification of mycorrhizae and rusts. Mol.Ecol. 2: 113–118. http://dx.doi.org/10.1111/j.1365-294X.1993.tb00005.xCrossrefGoogle Scholar

  • [8] Girlanda M., Selosse M.A., Cafasso D., Brilli F., Delfine S., Fabbian R., Ghignone S., Pinelli P., Segreto R., Loreto F., Cozzolino S. & Perotto S. 2006 Inefficient photosynthesis in the Mediterranean orchid Limodorum abortivum is mirrored by specific association to ectomycorrhizal Russulaceae. Mol. Ecol. 15: 491–504. http://dx.doi.org/10.1111/j.1365-294X.2005.02770.xCrossrefGoogle Scholar

  • [9] Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 41: 95–98. Google Scholar

  • [10] Kjøller R. & Rosendahl S. 2000. Detection of arbuscular mycorrhizal fungi (Glomales) in roots by nested PCR and SSCP (single strand conformation polymorphism). Plant Soil 226: 189–196. http://dx.doi.org/10.1023/A:1026499923717CrossrefGoogle Scholar

  • [11] Kristiansen K.A., Taylor D.L., Kjøller R., Rasmussen H.N. & Rosendahl S. 2001. Identification of mycorrhizal fungi from single pelotons of Dactylorhiza majalis (Orchidaceae) using single-strand conformation polymorphism and mitochondrial ribosomal large subunit DNA sequences. Mol. Ecol. 10: 2089–2093. http://dx.doi.org/10.1046/j.0962-1083.2001.01324.xCrossrefGoogle Scholar

  • [12] Kristiansen K.A., Freudenstein J.V., Rasmussen F.N. & Rasmussen H.N. 2004. Molecular identification of mycorrhizal fungi in Neuwiedia veratrifolia (Orchidaceae). Mol. Phylogenet. Evol. 33: 251–258. http://dx.doi.org/10.1016/j.ympev.2004.05.015CrossrefGoogle Scholar

  • [13] Leake J.R. 1994. The biology of myco-heterotrophic (’saprophytic’) plants. New Phytol. 127: 171–216. http://dx.doi.org/10.1111/j.1469-8137.1994.tb04272.xCrossrefGoogle Scholar

  • [14] Masuhara G. & Katsuya K. 1991. Fungal coil formation of Rhizoctonia repens in seedlings of Galeola septentrionalis (Orchidaceae). Bot. Mag. Tokyo 104: 275–281. http://dx.doi.org/10.1007/BF02488381CrossrefGoogle Scholar

  • [15] Masuhara G., Katsuya K. & Yamaguchi K. 1993. Potential for symbiosis of Rhizoctonia solani and binucleate Rhizoctonia with seeds of Spiranthes amoena var. amoena in vitro. Mycol. Res. 97: 746–752. http://dx.doi.org/10.1016/S0953-7562(09)80156-1CrossrefGoogle Scholar

  • [16] McCormick M.K., Whigham D.F. & O’Neill J. 2004. Mycorrhizal diversity in photosynthetic terrestrial orchids. New Phytol. 163: 425–438. http://dx.doi.org/10.1111/j.1469-8137.2004.01114.xCrossrefGoogle Scholar

  • [17] McKendrick S.L., Leake J.R., Taylor D.L. & Read D.J. 2002. Symbiotic germination and development of the myco-heterotrophic orchid Neottia nidus-avis in nature and its requirement for locally distributed Sebacina spp. New Phytol. 154:233–247. http://dx.doi.org/10.1046/j.1469-8137.2002.00372.xCrossrefGoogle Scholar

  • [18] Nilsson L.A. 1980. The pollination ecology of Dactylorhiza sambucina (Orchidaceae). Bot. Not. 133: 367–385. Google Scholar

  • [19] Otero J.T., Ackerman J.D. & Bayman P. 2002. Diversity and host specificity of endophytic Rhizoctonia -like fungi from tropical orchids. Am. J. Bot. 89: 1852–1858. http://dx.doi.org/10.3732/ajb.89.11.1852CrossrefGoogle Scholar

  • [20] Perkins A.J. & McGee P.A. 1995. Distribution of the orchid mycorrhizal fungus Rhizoctonia solani, in relation to it’s host, Pterostylis acuminata, in the field. Aust. J. Bot. 43: 565–575. http://dx.doi.org/10.1071/BT9950565CrossrefGoogle Scholar

  • [21] Peterson R.L., Uetake Y. & Zelmer C. 1998. Fungal symbioses with orchid protocorms. Symbiosis 25: 29–55. Google Scholar

  • [22] Rasmussen H.N. 1995. Terrestrial orchids: from seed to mycotrophic plant. Cambridge University Press New York, NY, USA. Google Scholar

  • [23] Selosse M.A., Weiss M., Jany J.L. & Tillier A. 2002. Communities and populations of sebacinoid Basidiomycetes associated with the achlorophyllous orchid Neottia nidus-avis (L.) L.C.M. Rich. and neighbouring tree ectomycorrhizae. Mol. Ecol. 11: 1831–1844. http://dx.doi.org/10.1046/j.1365-294X.2002.01553.xCrossrefGoogle Scholar

  • [24] Selosse M.A., Faccio A., Scappaticci G. & Bonfante P. 2004. Chlorophyllous and achlorophyllous specimens of Epipactis microphylla (Neottieae, Orchidaceae) are associated with ectomycorrhizal septomycetes, including truffles. Microb. Ecol. 47: 416–426. http://dx.doi.org/10.1007/s00248-003-2034-3CrossrefGoogle Scholar

  • [25] Shefferson R.P., Weiss M., Kull T. & Taylor D.L. 2005. High specificity generally characterizes mycorrhizal association in rare lady’s slipper orchids, genus Cypripedium. Mol. Ecol. 14: 613–626. http://dx.doi.org/10.1111/j.1365-294X.2005.02424.xCrossrefGoogle Scholar

  • [26] Shefferson R.P., Kull T. & Tali K. 2008. Mycorrhizal interactions of orchids colonizing Estonian mine tailing hills. Am. J. Bot. 95: 156–164. http://dx.doi.org/10.3732/ajb.95.2.156Web of ScienceCrossrefGoogle Scholar

  • [27] Smith S.E. & Read D.J. 1997. Mycorrhizal symbiosis. Academic Press, San Diego, CA, USA. Google Scholar

  • [28] Taylor D.L. & Bruns T.D. 1997. Independent, specialized invasions of ectomycorrhizal mutualism by two nonphotosynthetic orchids. Proc. Natl. Acad. Sci. USA 94: 4510–4515. http://dx.doi.org/10.1073/pnas.94.9.4510CrossrefGoogle Scholar

  • [29] Taylor D.L. & Bruns T.D. 1999. Community structure of ectomycorrhizal fungi in a Pinus muricata forest: minimal overlap between the mature forest and resistant propagule communities. Mol. Ecol. 8: 1837–1850. http://dx.doi.org/10.1046/j.1365-294x.1999.00773.xCrossrefGoogle Scholar

  • [30] Taylor D.L. & McCormick M.K. 2008. Internal transcribed spacer primers and sequences for improved characterization of basidiomycetous orchid mycorrhizas. New Phytol. 177: 1020–1033. http://dx.doi.org/10.1111/j.1469-8137.2007.02320.xCrossrefWeb of ScienceGoogle Scholar

  • [31] Taylor D.L., Bruns T.D., Leake J.R. & Read D.J. 2002. Mycorrhizal specificity and function in myco-heterotrophic plants, pp. 375–414. In: van der Heijden M.G.A.& Sanders R.I. (eds), The ecology of mycorrhizas. Springer Verlag, Berlin, Germany. Google Scholar

  • [32] Taylor D.L., Bruns T.D., Szaro T.M. & Hodges S.A. 2003. Divergence in mycorrhizal specialization within Hexalectris spicata (Orchidaceae), a nonphotosynthetic desert orchid. Am. J. Bot. 90: 1168–1179. http://dx.doi.org/10.3732/ajb.90.8.1168CrossrefGoogle Scholar

  • [33] Tutin T.G., Heywood V.H., Burges N.A., Moore D.M., Valentine D.H., Walters S.M. & Webb D.A. 1980. Flora Europea. Cambridge Univ. Press, Cambridge, UK. Google Scholar

About the article

Published Online: 2009-09-10

Published in Print: 2009-10-01


Citation Information: Biologia, Volume 64, Issue 5, Pages 893–897, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-009-0175-7.

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© 2009 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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