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Volume 65, Issue 3


Ethanol fermentation of mahula (Madhuca latifolia) flowers using free and immobilized bacteria Zymomonas mobilis MTCC 92

Shuvashish Behera / Rama Mohanty / Ramesh Ray
  • Microbiology Laboratory, Central Tuber Crops Research Institute (Regional Centre), Bhubaneswar, 751019, Orissa, India
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Published Online: 2010-04-24 | DOI: https://doi.org/10.2478/s11756-010-0041-7


Mahula (Madhuca latifolia L.) is a deciduous tree commonly found in the tropical rain forests of Asian and Australian continent. Corolla, the edible part of its flowers, is rich in fermentable sugar (37 ± 0.23%; on dry weight basis). Batch fermentation of mahula flowers was carried out using Zymomonas mobilis MTCC 92 free cells and cells immobilized in calcium alginate matrix. The ethanol productions were 122.9 ± 0.972 and 134.6 ± 0.104 g/kg flowers on dry weight basis using free and immobilized cells, respectively, after 96 h of fermentation, which showed that cells entrapped in calcium alginate matrix yielded 8.7% more ethanol than free cells. Further, the immobilized cells were physiologically active up to three more cycles of fermentation producing 132.7 ± 0.095, 130.5 ± 0.09 and 128.7 ± 0.056 g ethanol per kg flower in first, second and third cycle, respectively.

Keywords: bio-ethanol; cell immobilization; fermentation; Madhuca latifolia; Zymomonas mobilis

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About the article

Published Online: 2010-04-24

Published in Print: 2010-06-01

Citation Information: Biologia, Volume 65, Issue 3, Pages 416–421, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-010-0041-7.

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© 2010 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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