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Biologia




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Volume 66, Issue 1

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Assessment of genetic variability among selected species of Apocynaceae

Tariq Mahmood
  • Plant Biochemistry and Molecular Biology Laboratory, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, PK-45320, Pakistan
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/ Anna Iqbal
  • Plant Biochemistry and Molecular Biology Laboratory, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, PK-45320, Pakistan
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/ Nazia Nazar
  • Plant Biochemistry and Molecular Biology Laboratory, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, PK-45320, Pakistan
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/ Ishrat Naveed / Bilal Abbasi / Syed Naqvi
Published Online: 2010-12-21 | DOI: https://doi.org/10.2478/s11756-010-0134-3

Abstract

Family Apocynaceae is an economically important family grown as ornamental plants and many wild species have medicinal uses as well. The aim of the present study was to understand the level and pattern of genetic variability among the selected individuals of Apocynaceae. For this purpose, three species of different genera of Apocynaceae, Thevetia peruviana, Alstonia scholaris and Catharanthus roseus, were collected from Rawalpindi and Quaid-i-Azam University forest, Islamabad. To evaluate the level of polymorphism within the species and members of different species, randomly amplified polymorphic DNA (RAPD) markers were used. A series of OPC RAPD primers were used; only six primers of OPC series gave amplification. Highest genetic variation at interspecific and intraspecific levels was shown by OPC 9 and the lowest polymorphism was observed in OPC 4. The data was analyzed by using software Statistica 5.5. In total 105 monomorphic and 272 polymorphic bands were produced from all primers. Therefore, out of 322 amplified products, 26% were monomorphic and 68% were polymorphic. Low genetic diversification was observed both at intraspecific and interspecific level. At the molecular level Alstonia scholaris and Catharanthus roseus (subfamily Plumerioideae) appeared in a group and Thevetia peruviana (subfamily Rauvolfoideae) formed another group, confirming the classification based on morphological characters.

Keywords: Apocynaceae; genetic diversity; RAPD

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About the article

Published Online: 2010-12-21

Published in Print: 2011-02-01


Citation Information: Biologia, Volume 66, Issue 1, Pages 64–67, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-010-0134-3.

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© 2011 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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