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Biologia




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Volume 66, Issue 2

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Rapid differentiation of phenotypically and genotypically similar Synechococcus elongatus strains by PCR fingerprinting.

Gangatharan Muralitharan
  • Department of Microbiology, School of Life Sciences, Bharathidasan University, Palkalaiperur, Tiruchirappalli, 620 024, Tamilnadu, India
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/ Nooruddin Thajuddin
  • Department of Microbiology, School of Life Sciences, Bharathidasan University, Palkalaiperur, Tiruchirappalli, 620 024, Tamilnadu, India
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Published Online: 2011-02-20 | DOI: https://doi.org/10.2478/s11756-011-0003-8

Abstract

PCR amplification techniques viz., repetitive DNA element PCR (REP-PCR), short tandemly repeated repetitive PCR (STRR-PCR) and arbitrarily primed PCR (RAPD-PCR) were used for the taxonomic discrimination among the strains of the unicellular cyanobacterium Synechococcus elongatus collected across the coastal regions of the Indian subcontinent. These strains showed similar phenotypic and genotypic characteristics. Data obtained from genomic fingerprinting were used to perform cluster analysis and demonstrated ability to differentiate strains at intra-specific level. Polymorphisms of different PCR amplification products can serve as strain-specific molecular fingerprints. In comparison with the STRR and RAPD, the REP primer set generates fingerprints of lower complexity, but still the phenogram clearly differentiated the strains. In conclusion, described PCR fingerprinting methods can be considered as promising tools for the differentiation at the strain level of cyanobacteria from the same species.

Keywords: Synechococcus elongatus; genetic diversity; PCR-fingerprinting; RAPD-PCR; REP-PCR; STRR-PCR

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About the article

Published Online: 2011-02-20

Published in Print: 2011-04-01


Citation Information: Biologia, Volume 66, Issue 2, Pages 238–243, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-011-0003-8.

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© 2011 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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