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Biologia




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Volume 66, Issue 2

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Cloning and expression of β-glucosidase gene from Bacillus licheniformis into E. coli BL 21 (DE3)

Sana Zahoor / Muhammad Javed / Muhammad Aftab / Ikram-ul-Haq
Published Online: 2011-02-20 | DOI: https://doi.org/10.2478/s11756-011-0020-7

Abstract

A 1.4 Kb fragment of Bacillus licheniformis ATCC 14580 encoding β-glucosidase was cloned and expressed in Escherichia coli. β-Glucosidase expressed by E. coli harboring cloned gene was located entirely in the intracellular fraction. Recombinant β-glucosidase protein was purified to homogeneity level and the molecular weight was found to be 53 kDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. It gave maximum activity at 50°C and pH 6. K m and V max were 0.206 mM and 1.26 U/mg, respectively, with p-nitrophenyl-β-D-glucopyranoside, while activation energy Ea, enthalpy of activation ?H and entropy of activation ΔS were found to be 66.31 kJ/mol, 64.04 kJ/mol and 48.28 J/mol/K, respectively. The pKa1 and pKa2 of the ionizable groups of active site residues involved in Vmax were found to be 5.5 and 7.0, respectively. When the recombinant β-glucosidase protein was used as a member of consortium with endoglucanase and exoglucanase for the saccharification of wheat straw, 123% increase in saccharification was observed.

Keywords: β-glucosidase; Bacillus licheniformis; cloning; expression

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About the article

Published Online: 2011-02-20

Published in Print: 2011-04-01


Citation Information: Biologia, Volume 66, Issue 2, Pages 213–220, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-011-0020-7.

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© 2011 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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