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Biologia

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Volume 68, Issue 4

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Serum lipoprotein profile and oxidative stress biomarkers in Wistar rats fed drinking water containing iron and copper

Alexey Tinkov
  • Department of Biological Chemistry, Orenburg State Medical Academy; 6, Sovetskaya Street, Orenburg, 460000, Russia
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/ Alexandr Nikonorov
  • Department of Biological Chemistry, Orenburg State Medical Academy; 6, Sovetskaya Street, Orenburg, 460000, Russia
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Published Online: 2013-06-13 | DOI: https://doi.org/10.2478/s11756-013-0213-3

Abstract

The aim of the research was to estimate the effect of different doses and combinations of iron and copper consumption with drinking water on lipid profile and oxidative stress biomarkers in albino Wistar rats serum. Rats were given drinking water containing 3 mg L−1 and 6 mg L−1 iron; copper 4.88 and 9.76 mg L−1; a mixture of 3 mg L−1 iron and 4.88 mg L−1 copper. Control group obtained pure drinking water. Total cholesterol, lipoprotein spectrum and markers of lipid and protein oxidation were analyzed. It has been seen that administration of iron in concentration of 6 mg L−1 induces lipid peroxidation and protein oxidation, while copper given in the maximal doses leads only to protein oxidation. Free radical oxidation in rats obtaining combination of iron and copper with drinking water was more expressed than in case of administration of single metals in the same doses. Consumption of maximal doses of isolated metals leads to more expressed atherogenic changes, while combination of both metals in lower doses did not affect serum lipoprotein significantly. The data obtained show that chemical interaction of iron and copper in the organism has an additive effect on some vital parameters in comparison to isolated metal administration.

Keywords: iron; copper; oxidative stress; dyslipoproteinemia

  • [1] Abuja P.M. & Albertini R. 2001. Methods for monitoring oxidative stress, lipid peroxidation and oxidant resistance of lipoproteins. Clin. Chim. Acta 306: 1–17. DOI: 10.1016/S0009-8981(01)00393-X http://dx.doi.org/10.1016/S0009-8981(01)00393-XCrossrefGoogle Scholar

  • [2] Arredondo M., Martinez R., Nunez M.T., Ruz M. & Olivares M. 2006. Inhibition of iron and copper uptake by iron, copper and zinc. Biol. Res. 39(1): 95–102. DOI: 10.4067/S0716-97602006000100011 http://dx.doi.org/10.4067/S0716-97602006000100011CrossrefGoogle Scholar

  • [3] Arredondo M., Munoz P., Mura C.V. & Nunez M.T. 2003. DMT1, a physiologically relevant apical Cu1+ transporter of intestinal cells. Am. J. Physiol. Cell. Physiol. 284: 1525–1530. DOI:10.1152/ajpcell.00480.2002 http://dx.doi.org/10.1152/ajpcell.00480.2002CrossrefGoogle Scholar

  • [4] Aust S.D., Morehouse L.A. & Thomas C.E. 1985. Role of metals in oxygen radical reactions. J. Free Radic. Biol. Med. 1(1): 3–25. DOI: 10.1016/0748-5514(85)90025-X http://dx.doi.org/10.1016/0748-5514(85)90025-XCrossrefGoogle Scholar

  • [5] Chaitanya K.V., Pathan A.A.K., Mazumdar S.S., Chakravarthi G.P., Parine N. & Bobbarala V. 2010. Role of oxidative stress in human health: an overview. J. Pharm. Res. 3(6): 1330–1333. Google Scholar

  • [6] Chevion M. 1988. A site-specific mechanism for free radical induced biological damage: the essential role of redox-active transition metals. Free Radic. Biol. Med. 5(1): 27–37. DOI: 10.1016/0891-5849(88)90059-7 http://dx.doi.org/10.1016/0891-5849(88)90059-7CrossrefGoogle Scholar

  • [7] Dousset N., Ferretti G., Taus M., Valdiguie P. & Curatola G. 1994. Fluorescence analysis of lipoprotein peroxidation. Methods Enzymol. 233: 459–469. DOI: 10.1016/S0076-6879(94)33052-2 http://dx.doi.org/10.1016/S0076-6879(94)33052-2CrossrefGoogle Scholar

  • [8] Fenton H.J.H. 1894. Oxidation of tartaric acid in the presence of iron. J. Chem. Soc., Trans. 65: 899–910. DOI: 10.1039/CT8946500899 http://dx.doi.org/10.1039/ct8946500899CrossrefGoogle Scholar

  • [9] Durackova Z. 2010. Some current insights into oxidative stress. Physiol. Res. 59(4): 459–469. Google Scholar

  • [10] Friedewald W.T., Levy R.I. & Fredrickson D.S. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18(6): 499–502. Google Scholar

  • [11] Galhardi C.M., Diniz Y.S., Faine L.A., Rodrigues H.G., Burneiko R.C., Ribas B.O. & Novelli E.L. 2004. Toxicity of copper intake: lipid profile, oxidative stress and susceptibility to renal dysfunction. Food Chem. Toxicol. 42(12): 2053–2060. DOI:10.1016/j.fct.2004.07.020 http://dx.doi.org/10.1016/j.fct.2004.07.020CrossrefGoogle Scholar

  • [12] Giulivi C. & Davies K.J. 1994. Dityrosine: a marker for oxidatively modified proteins and selective proteolysis. Methods Enzymol. 233: 363–371. http://dx.doi.org/10.1016/S0076-6879(94)33042-5Google Scholar

  • [13] Graham R.M., Chua A.C., Carter K.W., Delima R.D., Johnstone D., Herbison C.E., Firth M.J., O’Leary R., Milward E.A., Olynyk J.K. & Trinder D. 2010. Hepatic iron loading in mice increases cholesterol biosynthesis. Hepatology 52(2): 462–471. DOI: 10.1002/hep.23712 http://dx.doi.org/10.1002/hep.23712CrossrefWeb of ScienceGoogle Scholar

  • [14] Jomova K. & Valko M. 2011. Thermodynamics of free radical reactions and the redox environment of a cell. Chapter 3, pp. 71–82. In: Andreescu S. & Hepel M. (eds), Oxidative Stress: Diagnostics, Prevention, and Therapy, ACS Symposium Series Vol. 1083; American Chemical Society: Washington, DC. 438 pp. ISBN-10: 0841226830, ISBN-13: 978-0841226838 Chapter DOI: 10.1021/bk-2011-1083.ch003 http://dx.doi.org/10.1021/bk-2011-1083.ch003Google Scholar

  • [15] Kato Y., Kitamoto N., Kawai Y. & Osawa T. 2001. The hydrogen peroxide/copper ion system, but not other metal-catalyzed oxidation systems, produces protein-bound dityrosine. Free Radic. Biol. Med. 31(5): 624–632. DOI: 10.1016/S0891-5849(01)00623-2 http://dx.doi.org/10.1016/S0891-5849(01)00623-2CrossrefGoogle Scholar

  • [16] Letelier M.E., Sánchez-Jofre S., Peredo-Silva L., Cortés-Troncoso J. & Aracena-Parks P. 2010. Mechanisms underlying iron and copper ions toxicity in biological systems: Pro-oxidant activity and protein-binding effects. Chem. Biol. Interact. 188(1): 220–227. DOI:10.1016/j.cbi.2010.06.013 http://dx.doi.org/10.1016/j.cbi.2010.06.013CrossrefWeb of ScienceGoogle Scholar

  • [17] Levine R.L., Garland D., Oliver C.N., Amici A., Climent I., Lenz A.G., Ahn B.W., Shaltiel S. & Stadtman E.R. 1990. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol. 186: 464–478. http://dx.doi.org/10.1016/0076-6879(90)86141-HGoogle Scholar

  • [18] Lowry O.H., Rosebrough N.J., Farr A.L. & Randall R.J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193(1): 265–275. Google Scholar

  • [19] Lynch S.M. & Frei B. 1993. Mechanisms of copper- and iron-dependent oxidative modification of human low density lipoprotein. J. Lipid Res. 34(10): 1745–1753. Google Scholar

  • [20] Meerson F.Z., Tverdokhlib V.P., Nikonorov A.A., Filippov V.K. & Frolov B.A. 1988. Rolj podavleniya aktivnosti pechenochnoĭ holesterin-7-aljfa-gidroksilazi v razvitii aterogennoy stressornoy dislipidemii [The role of suppression of cholesterol 7-hydroxylase activity of the liver in the development of atherogenic stress-induced dyslipoproteinemia]. Kardiologiya 28(9): 85–87. PMID: 3236653 Google Scholar

  • [21] Ohguchi S., Ichimiya H., Yagi A., Hayashi H. & Sakamoto N. 1988. Copper-induced hypercholesterolemia of golden hamsters: enhanced synthesis of cholesterol in the liver. Gastroenterol. Jpn. 23(6): 629–632. DOI: 10.1007/BF02782947 CrossrefGoogle Scholar

  • [22] Ohkawa H., Ohishi N. & Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95(2): 351–358. DOI: 10.1016/0003-2697(79)90738-3 http://dx.doi.org/10.1016/0003-2697(79)90738-3CrossrefGoogle Scholar

  • [23] Sharp P. 2004. The molecular basis of copper and iron interactions. Joint Meeting of the Nutrition-Society/Food-Standards-Agency, London, England, Feb. 11–12, 2004. Proc. Nutr. Soc. 63(4): 563–569. DOI: 10.1079/PNS2004386 http://dx.doi.org/10.1079/PNS2004386CrossrefGoogle Scholar

  • [24] Stadler N., Lindner R.A. & Davies M.J. 2004. Direct detection and quantification of transition metal ions in human atherosclerotic plaques: evidence for the presence of elevated levels of iron and copper. Arterioscler. Thromb. Vasc. Biol. 24(5): 949–954. DOI: 10.1161/01.ATV.0000124892.90999.cb http://dx.doi.org/10.1161/01.ATV.0000124892.90999.cbPubMedCrossrefGoogle Scholar

  • [25] Steinberg D. 1997. Low density lipoprotein oxidation and its pathobiological significance. J. Biol. Chem. 272(34): 20963–20966. DOI:10.1074/jbc.272.34.20963 http://dx.doi.org/10.1074/jbc.272.34.20963CrossrefGoogle Scholar

  • [26] Stohs S.J. & Bagchi D. 1995. Oxidative mechanisms in the toxicity of metal ions. Free Radic. Biol. Med. 18(2): 321–336. DOI: 10.1016/0891-5849(94)00159-H http://dx.doi.org/10.1016/0891-5849(94)00159-HCrossrefGoogle Scholar

  • [27] Tinkov A.A., Ajsuvakova O.P., Shehtman A.M., Boev V.M. & Nikonorov A.A. 2012. Influence of iron and copper consumption on weight gain and oxidative stress in adipose tissue of Wistar rats. Interdiscip. Toxicol. 5(3): 127–132. DOI: 10.2478/v10102-012-0021-6 http://dx.doi.org/10.2478/v10102-012-0021-6CrossrefGoogle Scholar

  • [28] Valko M., Morris H. & Kronin M.T.D. 2005. Metals, toxicity and oxidative stress. Cur. Med. Chem. 12(10): 1161–1208. PMID: 15892631 http://dx.doi.org/10.2174/0929867053764635Google Scholar

  • [29] Vogiatzi G., Tousoulis D. & Stefanadis C. 2009. The role of oxidative stress in atherosclerosis. Hellenic J. Cardiol. 50(5): 402–409. Google Scholar

  • [30] Witztum J.L. & Steinberg D. 1991. Role of oxidized low density lipoprotein in atherogenesis. J. Clin. Invest. 88(6): 1785–1792. DOI: 10.1172/JCI115499 http://dx.doi.org/10.1172/JCI115499CrossrefGoogle Scholar

About the article

Published Online: 2013-06-13

Published in Print: 2013-08-01


Citation Information: Biologia, Volume 68, Issue 4, Pages 738–742, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-013-0213-3.

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© 2013 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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