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Volume 68, Issue 5 (Oct 2013)


Molecular and morphological characterization of Pristionchus pacificus (Nematoda: Rhabditida: Neodiplogastridae), a new record of an entomophilic nematode from Iran

Mahnaz Hassani-Kakhki
  • Biocontrol and Insect Pathology Lab., Plant Protection Department, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • Email:
/ Javad Karimi
  • Biocontrol and Insect Pathology Lab., Plant Protection Department, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • Email:
/ Ebrahim Shokoohi
  • Plant Protection Department, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
  • Email:
Published Online: 2013-08-31 | DOI: https://doi.org/10.2478/s11756-013-0232-0


During a survey of entomopathogenic nematodes from the Mashhad region, in northeastern Iran, an entomophilic nematode was isolated using the Galleria trap method. Morphological characterization showed that this isolate belongs to the family Neodiplogastridae. Detailed morphological and morphometric characterization including SEM data fit with Pristionchus pacificus Sommer, Carta, Kim et Sternberg, 1996, representing the first record of this genus and species from Iran. Iranian specimens of P. pacificus FUM 5 are characterized by the body length (0.69–0.83 mm), the stoma (6–10 μm) bearing a dorsal tooth and a conical elongated tail (146–220 μm). Molecular analyses using 18S and ITS rDNA genes further support this identification. Measurements, illustrations, and SEM photographs of the Iranian isolate are provided and the phylogenetic position of the species is discussed.

Keywords: Pristionchus pacificus; SEM; ITS; 18S rDNA; Iran

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About the article

Published Online: 2013-08-31

Published in Print: 2013-10-01

Citation Information: Biologia, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-013-0232-0. Export Citation

© 2013 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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