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Volume 68, Issue 5 (Oct 2013)

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Molecular and morphological characterization of Pristionchus pacificus (Nematoda: Rhabditida: Neodiplogastridae), a new record of an entomophilic nematode from Iran

Mahnaz Hassani-Kakhki
  • Biocontrol and Insect Pathology Lab., Plant Protection Department, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • Email:
/ Javad Karimi
  • Biocontrol and Insect Pathology Lab., Plant Protection Department, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
  • Email:
/ Ebrahim Shokoohi
  • Plant Protection Department, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
  • Email:
Published Online: 2013-08-31 | DOI: https://doi.org/10.2478/s11756-013-0232-0

Abstract

During a survey of entomopathogenic nematodes from the Mashhad region, in northeastern Iran, an entomophilic nematode was isolated using the Galleria trap method. Morphological characterization showed that this isolate belongs to the family Neodiplogastridae. Detailed morphological and morphometric characterization including SEM data fit with Pristionchus pacificus Sommer, Carta, Kim et Sternberg, 1996, representing the first record of this genus and species from Iran. Iranian specimens of P. pacificus FUM 5 are characterized by the body length (0.69–0.83 mm), the stoma (6–10 μm) bearing a dorsal tooth and a conical elongated tail (146–220 μm). Molecular analyses using 18S and ITS rDNA genes further support this identification. Measurements, illustrations, and SEM photographs of the Iranian isolate are provided and the phylogenetic position of the species is discussed.

Keywords: Pristionchus pacificus; SEM; ITS; 18S rDNA; Iran

  • [1] Abebe E., Mekete T. & Thomas W.K. 2011. A critique of current methods in nematode taxonomy. Afr. J. Biotechnol. 10: 312–323. DOI: 10.5897/AJB10.1473 [Crossref]

  • [2] Bedding R.A. & Akhurst R.J. 1975. A simple technique for the detection of insect parasitic rhabditid nematodes in soil. Nematologica 21: 109–110. DOI: 10.1163/187529275X00419 http://dx.doi.org/10.1163/187529275X00419 [Crossref]

  • [3] Blaxter M. 2003. Counting angels with DNA. Nature 421: 122–124. DOI: 10.1038/421122a http://dx.doi.org/10.1038/421122a [Crossref]

  • [4] Blaxter M.L., De Ley P., Garey, J.R., Liu L.X., Scheldeman P., Vierstraete A., Vanfleteren J.R., Mackey L.Y., Dorris M., Frisse L.M., Vida J.T. & Thomas K. 1998. A molecular evolutionary framework for the phylum Nematoda. Nature 392: 71–75. DOI: 10.1038/32160 http://dx.doi.org/10.1038/32160 [Crossref]

  • [5] Brown F.D., D’anna I. & Sommer R.J. 2011. Host-finding behaviour in the nematode Pristionchus pacificus. Proc.R. Soc. B. 278: 3260–3269 DOI: 10.1098/rspb.2011.0129 http://dx.doi.org/10.1098/rspb.2011.0129 [Crossref] [Web of Science]

  • [6] Caldwell J.R. 1981. Biomass and respiration of nematode populations in two moss communities at Signy Island, Maritime Antarctic. Oikos 37: 160–166. http://dx.doi.org/10.2307/3544460 [Crossref]

  • [7] Click A., Savaliya C.H., Kienle S., Hermann M. & Pires-Dasilva A. 2009. Natural variation of outcrossing in the hermaphroditic nematode Pristionchus pacificus. BMC Evol. Biol. 9: 75. DOI: 10.1186/1471-2148-9-75 http://dx.doi.org/10.1186/1471-2148-9-75 [Web of Science] [Crossref]

  • [8] De Grisse A.T. 1969. Redscription ou modification de quelques techniques utilises dans l’etude des nematodes phytoparasitaries. Meded. Rijks Facultteit der Landbouwe Tenschappen Gent 34: 351–369.

  • [9] De Ley P., De Ley I., Morris K., Abebe E., Mundo M., Yoder M., Heras J., Waumann D., Rocha-olivares A., Burr J., Baldwin J. & Thomas W. 2005. An integrated approach to fast and informative morphological vouchering of nematodes for applications in molecular barcoding. Phil. Trans. R. Soc. B. 360: 1945–1958. DOI: 10.1098/rstb.2005.1726 http://dx.doi.org/10.1098/rstb.2005.1726 [Crossref]

  • [10] De Ley P., De Velde M.C., Mounport D., Baujard P. & Coomans A. 1995. Ultrastructure of the stoma in Cephalobidae, Panagrolaimidae and Rhaditidae, with a proposal for a revised stomaterminology in Rhabditida (Nematoda). Nematologica 41(4–5): 153–182. DOI: 10.1163/003925995X00143 http://dx.doi.org/10.1163/003925995X00143 [Crossref]

  • [11] De Man J.G. 1912. Odontopharynx longicaudata n. g. n. sp. Eine neue from von Anguilluliden. Zool. Jahrb. Jena Abt. Syst. 33: 637–642.

  • [12] Dereeper A., Guignon V., Blanc G., Audic S., Bufet S., Chevenet F., Dufayard J.-F., Guindon S., Lefort V., Lescot M., Claverie J.M. & Gascuel O. 2008. Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 36: W465–W469. DOI: 10.1093/nar/gkn180 http://dx.doi.org/10.1093/nar/gkn180 [Web of Science] [Crossref]

  • [13] Dorris M., De Ley P. & Blaxter M.L. 1999. Molecular analysis of nematode diversity and the evolution of parasitism. Parasitol. Today 15: 188–193. DOI: 10.1016/S0169-4758(99)01439-8 http://dx.doi.org/10.1016/S0169-4758(99)01439-8 [Crossref]

  • [14] Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791. DOI: 10.2307/2408678 http://dx.doi.org/10.2307/2408678 [Crossref]

  • [15] Floyd R., Eyualem A., Papert A. & Blaxter M. 2002. Molecular barcodes for soil nematode identification. Mol. Ecol. 11: 839–850. DOI: 10.1046/j.1365-294X.2002.01485.x http://dx.doi.org/10.1046/j.1365-294X.2002.01485.x [Crossref]

  • [16] Herrmann M., Mayer W.E., Hong R.L., Kienle S., Minasaki R. & Sommer R.J. 2007. The nematode Pristionchus pacificus (Nematoda: Diplogastridae) is associated with the oriental beetle Exomala orientalis (Coleoptera: Scarabaeidae) in Japan. Zool. Sci. 24: 883–889. DOI: 0289-0003-24-9-883 http://dx.doi.org/10.2108/zsj.24.883 [Crossref] [Web of Science]

  • [17] Hong R.L. & Sommer R.J. 2006. Pristionchus pacificus: a wellrounded nematode. Bioessays 28: 651–659. DOI: 10.1002/bies.20404 http://dx.doi.org/10.1002/bies.20404 [Crossref]

  • [18] Hong R.L., Svatos A., Herrmann M. & Sommer R.J. 2008a. The species-specific recognition of beetle cues by the nematode Pristionchus maupasi. Evol. Dev. 10(3): 273–279. http://dx.doi.org/10.1111/j.1525-142X.2008.00236.x [Crossref] [Web of Science]

  • [19] Hong R.L., Witte H. & Sommer R.J. 2008b. Natural variation in Pristionchus pacificus insect pheromone attraction involves the protein kinase EGL-4. Proc. Natl. Acad. Sci. U.S.A. 105: 7779–7784. DOI: 10.1073/pnas.0708406105 http://dx.doi.org/10.1073/pnas.0708406105 [Crossref]

  • [20] Kanzaki N., Taki H. Masuya H., Okabe K. Tanaka R. & Abe F. 2011. Diversity of stag beetle-associated nematodes in Japan. Environ. Entomol. 40: 281–288. DOI: 10.1603/EN10182 http://dx.doi.org/10.1603/EN10182 [Web of Science] [Crossref]

  • [21] Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111–120. DOI: 10.1007/BF01731581 http://dx.doi.org/10.1007/BF01731581 [Crossref]

  • [22] Kreis H.A. 1932. Beiträge zur Kenntnis pflanzenparasitischer Nematoden. Zeitschr. Parasitenkd. 5: 184–194. DOI: 10.1007/BF02120641 http://dx.doi.org/10.1007/BF02120641 [Crossref]

  • [23] Kumari S. & Liskova M. 2009. Molecular confirmation of Xiphinema italiae Meyl, 1953 (Nematoda: Longidoridae) from the Slovak Republic. Helminthologia 46: 131–134. DOI: 10.2478/s11687-009-0025-8 http://dx.doi.org/10.2478/s11687-009-0025-8 [Web of Science] [Crossref]

  • [24] Mayer W.E., Herrmann M. & Sommer R.J. 2007. Phylogeny of the nematode genus Pristionchus and implications for biodiversity, biogeography and the evolution of hermaphroditism. BMC Evol. Biol. 7: 104. DOI: 10.1186/1471-2148-7-104 http://dx.doi.org/10.1186/1471-2148-7-104 [Web of Science] [Crossref]

  • [25] Mayer W.E., Herrmann M. & Sommer R.J. 2009. Molecular phylogeny of beetle associated diplogastrid nematodes suggests host switching rather than nematode-beetle coevolution. BMC Evol. Biol.9: 212. DOI: 10.1186/1471-2148-9-212 http://dx.doi.org/10.1186/1471-2148-9-212 [Crossref] [Web of Science]

  • [26] Morgank K., Mcgaughran A., Villate L., Herrmann M., Witte H., Bartelmes G., Rochat J. & Sommer R.J. 2012. Multi locus analysis of Pristionchus pacificus on La Re’union Island reveals an evolutionary history shaped by multiple introductions, constrained dispersal events and rare out-crossing. Mol. Ecol. 21: 250–266. DOI: 10.1111/j.1365-294X.2011.05382.x http://dx.doi.org/10.1111/j.1365-294X.2011.05382.x [Crossref] [Web of Science]

  • [27] Rae R., Riebesell M., Dinkelacker I., Wang O., Herrmann M., Weller A.M., Dieterich C. & Sommer R.J. 2008. Isolation of naturally associated bacteria of necromenic Pristionchus nematodes and fitness consequences. J. Exp. Biol. 211: 1927–1936. DOI: 10.1242/jeb.014944 http://dx.doi.org/10.1242/jeb.014944 [Crossref] [Web of Science]

  • [28] Rudel D., Riebesell M. & Sommer R.J. 2005. Gonadogenesis in Pristionchus pacificus and organ evolution: development, adult morphology and cell-cell interactions in the hermaphrodite gonad. Dev. Biol. 277: 200–221. DOI: 10.1016/j.ydbio.2004.09.021 http://dx.doi.org/10.1016/j.ydbio.2004.09.021 [Crossref]

  • [29] Saitou N. & Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–425. [Web of Science]

  • [30] Sudhaus W. & Furst V.L.A. 2003. A phylogenetic classification and catalogue of the Diplogastridae (Secernentea, Nematoda). J. Nematode Morp. Syst. 6: 43–90.

  • [31] Shokoohi E. & Abolafia J. 2011. New data and SEM observations of six known species of the superfamily Rhabditoidea Örley, 1880 (Rhabditida) from Tehran province (Iran). J. Nematode Morph. Syst. 14(1): 39–54.

  • [32] Sochova I., Hofman J. & Holoubek I. 2006. Using nematodes in soil ecotoxicology. Environ. Int. 32: 374–383. DOI: 10.1016/j.envint.2005.08.031 http://dx.doi.org/10.1016/j.envint.2005.08.031 [PubMed] [Crossref]

  • [33] Sommer R.J. 2006. Pristionchus pacificus. In: The C. elegans Research Community (eds), Worm Book. http://www.wormbook.org,doi/10.1895/wormbook.1.102.1. (accessed 05.03.2011)

  • [34] Sommer R.J., Carta L.K., Kim S.Y. & Stenberg P.W. 1996. Morphological, genetic and molecular description of Pristionchus pacificus sp. n. (Nematoda: Neodiplogasteridae). Fund. Appl. Nematol. 19: 511–521.

  • [35] Stock S.P. 2009. Molecular approaches and taxonomy of insect parasitic and pathogenic nematodes, pp. 70–98. In: Stock S.P., Vandeberg J., Boemare N. & Glazer I. (eds), Insect Pathogens: Molecular Approaches and Techniques. Wallingford, UK: CABI Publishing, 417 pp. ISBN: 9781845934781 1845934784 http://dx.doi.org/10.1079/9781845934781.0000

  • [36] Stock S.P., Campbell J.F. & Nadler S.A. 2001. Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) inferred from ribosomal DNA sequences and morphological characters. J. Parasitol. 87: 877–889. DOI: 10.2307/3285148 http://dx.doi.org/10.2307/3285148 [Crossref]

  • [37] Stock S.P. & Reid A.P. 2004. Biosystematics of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae): current status and future directions, pp. 435–446. In: Cook R. & Hunt D.J. (eds), Proceedings of the Fourth International Congress of Nematology, June 8–13, 2002, Tenerife, Spain, Nematology Monographs and Perspectives 2, Brill Academic Publ., 866 pp. ISBN13: 9789004139213

  • [38] Subbotin S., Sturhan D., Volvas N., Castillo P., Tanyi T., Moens M. & Baldwin J. 2007. Application of the secondary structure model of rRNA for phylogeny: D2-D3 expansion segments of the LSU gene of plant parasitic nematodes from the family Hoplolaimidae Filipjev, 1934. Mol. Phylogenet. Evol. 43: 881–890. DOI: 10.1016/j.ympev.2006.09.019 http://dx.doi.org/10.1016/j.ympev.2006.09.019 [Crossref] [Web of Science]

  • [39] Tamura K. & Nei M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10: 512–526. PMID: 8336541

  • [40] Tamura K., Dudley J., Nei M. & Kumar, S. 2007. MEGA4:Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24: 1596–1599. DOI: 10.1093/molbev/msm092 http://dx.doi.org/10.1093/molbev/msm092 [Crossref]

  • [41] Thompson J.D., Higgins D.G. & Gibsson T.J. 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673–4680. DOI: 10.1093/nar/22.22.4673 http://dx.doi.org/10.1093/nar/22.22.4673 [Crossref]

  • [42] Vrain T.C., Wakarchuk D.A., Levesque A.C. & Hamilton R.I. 1992. Intraspecific rDNA restriction fragment length polymorphisms in the Xiphinema americanum group. Fund. Appl. Nematol. 15: 563–574.

  • [43] Weller A.M., Mayer W.E., Rae R. & Sommer, R.J. 2010. Quantitative assessment of the nematode fauna present on Geotrupes dung beetles reveals species-rich communities with a heterogeneous distribution. J. Parasitol. 96: 525–531. DOI: http://dx.doi.org/10.1645/GE-2319.1 http://dx.doi.org/10.1645/GE-2319.1 [Crossref] [Web of Science]

  • [44] White G.F. 1927. A method for obtaining infective nematode larvae from cultures. Science 66: 302–303. DOI: 10.1126/science.66.1709.302-a http://dx.doi.org/10.1126/science.66.1709.302-a [Crossref]

  • [45] Zauner H., Mayer W., Herrmann M., Weller A., Erwig M. & Sommer R.J. 2007. Distinct patterns of genetic variation in Pristionchus pacificus and Caenorhabditis elegans, two partially selfing nematodes with cosmopolitan distribution. Mol. Ecol. 16: 1267–1280. DOI: 10.1111/j.1365-294x.2006.03222.x http://dx.doi.org/10.1111/j.1365-294X.2006.03222.x [Web of Science] [Crossref]

About the article

Published Online: 2013-08-31

Published in Print: 2013-10-01



Citation Information: Biologia, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.2478/s11756-013-0232-0. Export Citation

© 2013 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

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