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Biologia




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Volume 69, Issue 1

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Discrimination between four Simocephalus species from Slovakia using a PCR-RFLP technique

Jan Kohout
  • Institute of Zoology, Slovak Academy of Sciences, Dúbravská cesta 9, 84506, Bratislava, Slovakia
  • Faculty of Fisheries and Protection of Waters, University of South Bohemia, Zátiší 728/II, 38925, Vodňany, Czech Republic
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/ Marta Illyová / Fedor Čiampor / Zuzana Čiamporová-Zaťovičová
Published Online: 2013-11-15 | DOI: https://doi.org/10.2478/s11756-013-0285-0

Abstract

Planktonic crustaceans are traditionally identified based on morphological and morphometric characters. However, such characters may be hardly distinguishable and often overlap between species. A probability of misidentification is thus relatively high. Molecular techniques may increase the accuracy of identification if appropriate markers are used. Aim of our work was to develop a simple molecular procedure enabling discrimination between four species of Simocephalus occurring in Europe. PCR-RFLP technique proved to be suitable for such discrimination. Within the 709 bp fragment of mitochondrial cytochrome c oxidase subunit 1 gene we found unique combinations of restriction sites of the BbsI and SacI enzymes for Simocephalus vetulus, S. exspinosus, S. serrulatus and S. congener. PCR products of samples from several locations in Slovakia were digested with the two enzymes and electrophoresed on an agarose gel. The restriction patterns were clearly visible and easily distinguishable. This method is applicable for identifying the four species in any life-stage. Considering its simplicity and cost-effectiveness it can be widely used as a diagnostic tool for discriminating between Simocephalus species with overlapping morphologic characters.

Keywords: cytochrome oxidase I; Anomopoda; species identification; restriction fragment length polymorphism; Cladocera

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About the article

Published Online: 2013-11-15

Published in Print: 2014-01-01


Citation Information: Biologia, Volume 69, Issue 1, Pages 76–79, ISSN (Online) 1336-9563, DOI: https://doi.org/10.2478/s11756-013-0285-0.

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© 2013 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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