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Biologia

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Volume 69, Issue 6

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Expression of soluble Saccharomyces cerevisiae alcohol dehydrogenase in Escherichia coli applicable to oxido-reduction bioconversions

Pavol Utekal
  • Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina 1, SK-84215, Bratislava, Slovakia
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/ Csaba Tóth
  • Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina 1, SK-84215, Bratislava, Slovakia
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/ Anikó Illésová
  • Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina 1, SK-84215, Bratislava, Slovakia
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/ Pavol Koiš
  • Department of Organic Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina 1, SK-84215, Bratislava, Slovakia
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/ Lucia Bocánová
  • Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina 1, SK-84215, Bratislava, Slovakia
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/ Ján Turňa
  • Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina 1, SK-84215, Bratislava, Slovakia
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/ Hana Drahovská
  • Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina 1, SK-84215, Bratislava, Slovakia
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/ Stanislav Stuchlík
  • Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynská dolina 1, SK-84215, Bratislava, Slovakia
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Published Online: 2014-05-04 | DOI: https://doi.org/10.2478/s11756-014-0376-6

Abstract

Six-carbon aldehydes and alcohols belong to flavours and fragrances with wide application in the food, feed, cosmetic, chemical and pharmaceutical sectors. In the present study, we prepared the expression system for production of recombinant yeast alcohol dehydrogenase 1 (YADH1) from Saccharomyces cerevisiae which is suitable also for catalysis of the interconversion of C-6 aldehydes and alcohols. We have demonstrated that an effective three-step strategy can overcome the insolubility problems during YADH1 production in Escherichia coli. We used trxB and gor deficient expression strain, decreased concentration of isopropyl β-D-1-thiogalactopyranoside and lowered temperature to 20°C during induction. Finally, kinetic parameters of recombinant YADH1 were determined and we concluded it is a promising enzyme also for the interconversion of C-6 alcohols/aldehydes in green note volatile production.

Keywords: alcohol dehydrogenase; soluble protein expression; green notes bioconversion

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About the article

Published Online: 2014-05-04

Published in Print: 2014-06-01


Citation Information: Biologia, Volume 69, Issue 6, Pages 722–726, ISSN (Online) 1336-9563, DOI: https://doi.org/10.2478/s11756-014-0376-6.

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© 2014 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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