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Biologia




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Volume 69, Issue 8

Issues

Overexpression of DnWRKY11 enhanced salt and drought stress tolerance of transgenic tobacco

Xiang-Bin Xu / Yuan-Yuan Pan / Chun-Ling Wang / Qi-Cai Ying / Hong-Miao Song / Hui-Zhong Wang
Published Online: 2014-08-19 | DOI: https://doi.org/10.2478/s11756-014-0398-0

Abstract

Dendrobium seedlings showed low survival rate when they were transferred from in vitro conditions to greenhouse or field environment. One of the major reasons is their low tolerance to environmental changes. WRKY transcription factors are one of the largest families of transcriptional regulators in plants. They are involved in various biotic and abiotic stress responses. One DnWRKY11 gene was isolated from Dendrobium nobile. To explore the function of DnWRKY11 in Dendrobium defense responses to abiotic stress, it was overexpressed in tobacco. Under salt and drought stresses, the DnWRKY11 transgenic tobacco showed higher germination rate, longer root length, higher fresh weight, higher activities of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and lower content of malonidialdehyde (MDA) than the wild type. These results proved the important roles of DnWRKY11 in plant response to drought and salt stresses, and provided a potential gene for improving environmental stress tolerance of Dendrobium seedlings.

Keywords: Dendrobium nobile; abiotic stress; functional identification; WRKY

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About the article

Published Online: 2014-08-19

Published in Print: 2014-08-01


Citation Information: Biologia, Volume 69, Issue 8, Pages 994–1000, ISSN (Online) 1336-9563, DOI: https://doi.org/10.2478/s11756-014-0398-0.

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© 2014 Slovak Academy of Sciences. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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