Abstract
Symptoms of ash dieback have been recorded in Slovakia since 2004. The field sampling was carried out in 2013, included 59 segments of shoots and 10 and more petioles per locality from four localities. The causal agent of ash dieback, the hyphomycete Chalara fraxinea T. Kowalski, was isolated from Fraxinus excelsior L. from seven localities in Slovakia. The morphology of C. fraxinea isolates and the teleomorph Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz, Hosoya are described and ITS sequences are provided.
References
Bakys R., Vasaitis R., Barklund P., Ihrmark K. & Stenlid J. 2009. Investigations concerning the role of Chalara fraxinea in declining Fraxinus excelsior. Plant. Pathol. 58: 284-292.10.1111/j.1365-3059.2008.01977.xSearch in Google Scholar
Davydenko K., Vasaitis R., Stenlid J. & Menkis A. 2013 Fungi in foliage and shoots of Fraxinus excelsior in eastern Ukraine: a first report on Hymenoscyphus pseudoalbidus. For. Path. 43: 462-467.10.1111/efp.12055Search in Google Scholar
Baral H.O., Queloz V. & Hosoya T. 2014. Hymenoscyphus fraxineus, the correct scientific name for the fungus causing ash dieback in Europe. IMA Fungus 5: 79-80.Search in Google Scholar
Drenkhan R. & Hanso M. 2010. New host species for Chalara fraxinea. Plant Pathol. 22: 16.10.5197/j.2044-0588.2010.022.016Search in Google Scholar
Gardes M. & Bruns T.D. 1993. ITS primers with enhanced specifity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol. Ecol. 2: 113-118.10.1111/j.1365-294X.1993.tb00005.xSearch in Google Scholar PubMed
Halmschlager E. & Kirisits T. 2008. First report of the ash dieback pathogen Chalara fraxinea on Fraxinus excelsior in Austria. Plant Pathol. 57: 1177.10.1111/j.1365-3059.2008.01924.xSearch in Google Scholar
Husson C., Cäel O., Grandjean J.P., Nageleisen L.M. & Marçais B. 2012. Occurrence of Hymenoscyphus pseudoalbidus on infected ash logs. Plant Pathol. 61: 889-895.10.1111/j.1365-3059.2011.02578.xSearch in Google Scholar
Husson C., Scala B., Cäel O.; Frey P., Feau N., Ioos R. &Marçais B. 2011. Chalara fraxinea is an invasive pathogen in France. Eur. J. Plant Pathol. 130: 311-324.10.1007/s10658-011-9755-9Search in Google Scholar
Chandelier A., André F. & Laurent F. 2010. Detection of Chalara fraxinea in common ash (Fraxinus excelsior) using real time PCR. For. Path. 40: 87-95.10.1111/j.1439-0329.2009.00610.xSearch in Google Scholar
Jankovský L. & Holdenrieder O. 2009. Chalara fraxinea - ash dieback in the Czech Republic. Plant Protect. Sci. 45: 74-78.10.17221/45/2008-PPSSearch in Google Scholar
Johansson S.B.K., Vasaitis R., Ihrmark K., Barklund P. & Stenlid J. 2010. Detection of Chalara fraxinea from tissue of Fraxinus excelsior using species-speficic ITS primers. For. Path. 40: 111-115.10.1111/j.1439-0329.2009.00614.xSearch in Google Scholar
Kirisits T. & Cech T.L. 2010. Die Symptome des Eschentriebsterbens. http://bfw.ac.at/400/pdf/Eschentriebsterben2010.pdf.Search in Google Scholar
Kirisits T., Dämpfle L. & Kräutler K. 2013. Hymenoscyphus albidus is not associated with an anamorphic stage and displays slower growth than Hymenoscyphus pseudoalbidus on agar media. For. Pathol. 43: 386-389.10.1111/efp.12042Search in Google Scholar
Kirisits T., Kräutler K. & Cech T.L. 2010a. Wieder früher Blattfall der Esche [Again early leaf shedding of ash]. Forstzeitung 121: 30-31.Search in Google Scholar
Kirisits T., Matláková M., Mottinger-Kroupa S. & Halmschlager E. 2010b. Chalara fraxinea associated with dieback of narrowleafed ash (Fraxinus angustifolia). Plant Pathol. 59: 411.10.1111/j.1365-3059.2009.02162.xSearch in Google Scholar
Kirisits T., Matláková M., Mottinger-Kroupa S., Cech T.L. & Halmschlager E. 2009. The current situation of ash dieback caused by Chalara fraxinea in Austria, pp. 97-119. In: Dogmus-Lehtijärvi T. (ed.), Proceedings of the conference of IUFRO working party 7. 02. 02., Egirdir, Turkey, 11-16 May 2009. SDU Faculty Forestry Journal, ISSN: 1302-7085, Serial: A, Spec. Iss.Search in Google Scholar
Kowalski T. & Holdenrieder O. 2009. The teleomorph of Chalara fraxinea, the causal agent of ash dieback. For. Path. 39: 304-308.Search in Google Scholar
Kowalski T. & Łukomska A. 2005. Badania nad zamjeraniem jesionu (Fraxinus excelsior L.) w drzewostanach Nadl,Włoszczow. Acta Agrobotanica 59: 429-440.Search in Google Scholar
Kowalski T. 2006. Chalara fraxinea sp. now associated with dieback of ash (Fraxinus excelsior) in Poland. For. Path. 36: 264-270.10.1111/j.1439-0329.2006.00453.xSearch in Google Scholar
Kowalski T., Schumacher J. & Kehr R. 2010. Das Eschensterben in Europa - Symptome, Erreger und Empfehlungen für die Praxis, pp. 184-195. In: Dujesiefken D. (ed.), Jahrbuch der Baumpflege 2010. Haymarket Media, Braunschweig, Germany.Search in Google Scholar
Kräutler K. & Kirisits T. 2012. The ash dieback pathogen Hymenoscyphus pseudoalbidus is associated with leaf symptoms on ash species (Fraxinus spp.). J. Agric. Ext. Rural Dev. 4: 261-265.10.5897/JAERD12.065Search in Google Scholar
Kunca A, Leontovyč R., Zúbrik M. & Gubka A. 2011. Bark beetle outbreak on weakened ash trees and applied control measures. Bulletin OEPP. EPPO Bulletin 41: 11-13.10.1111/j.1365-2338.2010.02428.xSearch in Google Scholar
Kunca A. 2006. Occurrence of pest agents in Slovak forests in 2005 and prognosis for 2006. Lesnícky výskumný ústav, Zvolen, 15 pp. (in Slovak) Search in Google Scholar
Kunca A. 2007. Occurrence of pest agents in Slovak forests in 2006 and prognosis for 2007. Národné lesnícke centrum - Lesnícky výskumný ústav Zvolen, Zvolen, 71 pp. (in Slovak) Kunca A. 2008. Occurrence of pest agents in Slovak forests in 2007 and prognosis for 2008. Národné lesnícke centrum - Lesnícky výskumný ústav Zvolen, Zvolen, 70 pp. (in Slovak).Search in Google Scholar
Leontovyč R. & Kunca A. 2009. Increased dieback of broadleaved trees (ash, sweet chestnut, poplars) due to fungal pest agents, pp. 105-109. In: Kunca A. (ed.), Zborník referátov z medzinárodnej konferencie Aktuálne problémy v ochrane lesa 2008, 23-24 April 2009, Nový Smokovec. (in Slovak) Search in Google Scholar
OEPP/EPPO 2013. Diagnistics protocol PM 7/117 (1) Hymenoscyphus pseudoalbidus. Bulletin OEPP/EPPO 43: 449-461.10.1111/epp.12061Search in Google Scholar
Ogris N., Hauptmann T. & Jurc D. 2009. Chalara fraxinea causing common ash dieback newly reported in Slovenia. Plant Pathol. 58: 1173.10.1111/j.1365-3059.2009.02105.xSearch in Google Scholar
Queloz V., Grünig C., Berndt R., Kowalski T., Sieber T. N. & Holdenrieder O. 2011. Cryptic speciation in Hymenoscyphus albidus. For. Path. 41: 133-142.10.1111/j.1439-0329.2010.00645.xSearch in Google Scholar
Queloz V., Grünig C.R., Berndt R., Kowalski T., Sieber T.N. & Holdenrieder O. 2010. Cryptic speciation in Hymenoscyphus albidus. For. Path. 41: 133-142.10.1111/j.1439-0329.2010.00645.xSearch in Google Scholar
Schumacher J., Kehr R. & Leonhard S. 2010. Mycological and histological investigations of Fraxinus excelsior nursery saplings naturally infected by Chalara fraxinea. For. Path. 40: 419-429.10.1111/j.1439-0329.2009.00615.xSearch in Google Scholar
Schumacher J. 2011. The general situation regarding ash dieback in Germany and investigations concerning the invasion and distribution strategies of Chalara fraxinea in woody tissue.10.1111/j.1365-2338.2010.02427.xSearch in Google Scholar
EPPO Bulletin 40: 7-10.Search in Google Scholar
Schumacher J., Wulf A. & Leonhard S. 2007. Erster Nachweis von Chalara fraxinea T. Kowalski sp. nov. Deutschland - ein Verursacher neuartiger Schaden an Eschen. Nachr. bl. Dtsch.Search in Google Scholar
Pflanzenchutzd. 59: 121-123.Search in Google Scholar
Szabo I. 2008. A magas koris Chalara fraxinea okozta hajtasez vesszopusztulasanak megjelenese Magyaroszagon. N¨ovényvedelem 44: 444-446.Search in Google Scholar
Thomsen I.M., Skovsgaard J.P., Barklund P. & Vasaitis R. 2007. Svampesygdom er arsag til toptorre i ask. Skoven (Skov & Landskab; Kobenhavns Universitet; Copenhagen, DK) 5: 234-236.Search in Google Scholar
Timmermann V., Børja I., Hietala A. M., Kiristis T. & Solheim, H. 2011. Ash dieback: Pathogen spread and diurnal patterns of ascospore dispersal, with special emphasis on Norway. EPPO Bulletin 40: 14-20.Search in Google Scholar
White T. M., Bruns T., Lee S. & Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M. A., Gelfand D. H., Sninsky J. J. & White T. J. (eds), PCR protocols: A guide to methods and applications, Academic Press, New York.10.1016/B978-0-12-372180-8.50042-1Search in Google Scholar
Zhao Y., Hosoya T., Baral H., Hosaka K. & Kakishima M. 2012. Hymenoscyphus pseudoalbidus, the correct name for Lambertella albida reported from Japan. Mycotaxon 122: 25-41. Search in Google Scholar
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