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Biologia

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Volume 70, Issue 6

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Differential expression of apoptosis-associated genes in canine mammary tumors

Namita Mitra
  • Corresponding author
  • School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
 / Ramneek Verma
  • School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India
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  • De Gruyter OnlineGoogle Scholar
 / Dipak Deka
  • School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India
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  • De Gruyter OnlineGoogle Scholar
 / Hitesh N. Pawar
  • Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, USA
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 / Naresh K. Sood
  • Department of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
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 / Kuldeep Gupta
  • Department of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
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 / Shashi K. Mahajan
  • Department of Veterinary Surgery and Radiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
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  • De Gruyter OnlineGoogle Scholar
 / Jitender Mohindroo
  • Department of Veterinary Surgery and Radiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
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Published Online: 2016-01-08 | DOI: https://doi.org/10.1515/biolog-2015-0094

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Abstract

B-cell lymphoma 2 (Bcl-2) and heat shock protein (HSP) families are implicated in various processes of carcinogenesis, owing to their role in regulation of apoptosis and cell cycle, respectively. mRNA expression of Bcl-2, myeloid cell leukemia 1 (Mcl-1), B-cell lymphoma-extra-large (Bcl-xl), Bcl2-associated X (Bax), HSP70 and HSP90-β genes were studied in 30 clinical canine mammary tumors (CMTs). Histological ‘type’ and ‘grade’ were assigned to CMTs and expression was evaluated by SYBR-Green real-time PCR assay. Overall, the tumors exhibited the maximum expression of Bcl-2 amongst the Bcl-2 family members. Sarcoma and carcinosarcoma showed relatively higher expression of Mcl-1, whereas Bcl-2 was over-expressed in carcinoma. In relation to the cancer grades, Bcl-2/Bax ratio tend to increase as the tumor differentiation progressed from well to poorly differentiated. HSP90-β exhibited significantly high expression in carcinoma, carcinosarcoma and all grades of CMTs were suggestive of their elemental role in tumor progression. In conclusion, this study underpins the conjecture that Bcl-2, Mcl-1 and HSP90-β can be used as potential targets of inhibition in future mammary tumor therapeutics.

Keywords: canine mammary tumor; Bcl-2 family; HSP70; HSP90-β; apoptosis-associated genes

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About the article

Received: 2015-03-06

Accepted: 2015-06-08

Published Online: 2016-01-08

Published in Print: 2015-06-01

Citation Information: Biologia, Volume 70, Issue 6, Pages 846–852, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.1515/biolog-2015-0094.

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