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Biologia

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Volume 70, Issue 8 (Aug 2015)

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Mitochondrial structures during seed germination and early seedling development in Arabidopsis thaliana

José L. Rodríguez
  • Corresponding author
  • Departamento de Bioquímica y Biología Molecular, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain / IRNASA-CSIC, Cordel de Merinas, 40, 37080 Salamanca, Spain / Plant Developmental Genetics, Institute of Biophysics, The Czech Academy of Sciences, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Juana G. De Diego
  • Departamento de Bioquímica y Biología Molecular, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Francisco D. Rodríguez
  • Departamento de Bioquímica y Biología Molecular, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca, 37007 Salamanca, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Emilio Cervantes
Published Online: 2016-01-08 | DOI: https://doi.org/10.1515/biolog-2015-0130

Abstract

Mitochondrial morphology and evolution have been observed during seed germination and early seedling development in Arabidopsis thaliana line 43a9 (ecotype Columbia) expressing green fluorescent protein in these organelles. Fluorescence, confocal and electronic microscopy images reveal that mitochondrial development goes through different stages, and that the organelle structure varies with cell types during these processes. Mitochondria develop from larger, isodiametric structures pre-existent in the dry seed called promitochondria. After germination, variations in mitochondrial morphology occur synchronously with cell differentiation and cell division in the course of early root development. Some promitochondria develop into intermediate structures resembling the syncytial organelles. These structures have been described in certain plants under hypoxia as intermediates for the formation of mature mitochondria. On the other hand, other promitochondria temporarily remain in the cells of the root apex

This article offers supplementary material which is provided at the end of the article.

Keywords: Arabidopsis; confocal microscopy; germination; Mitotracker; promitochondria

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About the article

Received: 2015-02-26

Accepted: 2015-08-19

Published Online: 2016-01-08

Published in Print: 2015-08-01


Citation Information: Biologia, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.1515/biolog-2015-0130.

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