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Biologia




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Volume 71, Issue 11

Issues

Identification and molecular characterization of one novel 1Sl-encoded s-type low molecular weight glutenin B-subunit from 1Sl(1B) substitution line of wheat variety Chinese Spring (Triticum aestivum)

Xiong Deng / Shun-li Wang
  • Collegeof Life Science, Capital Normal University, Beijing 100048, China
  • Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Shou-min Zhen / Wen-ying Zhang
  • Hubei Collaborative Innovation Center for Grain Industry; Yangtze University, Jingzhou 434025, China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Yue-ming Yan
  • Corresponding author
  • Collegeof Life Science, Capital Normal University, Beijing 100048, China
  • Hubei Collaborative Innovation Center for Grain Industry; Yangtze University, Jingzhou 434025, China
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-12-24 | DOI: https://doi.org/10.1515/biolog-2016-0147

Abstract

Aegilops longissima (2n = 2x = 14, SlSl), has extensive allelic variations of glutenin subunits that are considered as useful gene sources for wheat quality improvement. The Chinese Spring 1Sl(1B) chromosome substitution line CS-1Sl(1B)showed superior dough properties and breadmaking quality due to the introgression of novel glutenin subunits encoded by 1Sl genome. In this study, we identified one novel 1Sl-encoded low molecular weight glutenin B-subunit 1SlLMW-s from CS-1Sl(1B). Its complete encoding sequences were isolated and designated as 1SlLMW-s with 960 bp encoding 318 amino acid residues. Molecular characterization demonstrated that the deduced 1SlLMW-s subunit had a rather large and regular repeated sequence domain, including a high proportion of glutamine residues (about 44%) in the repeats (consensus sequence PPFSQQQQ). A total of 31 SNPs were detected at different positions of encoding sequences. The secondary structure prediction revealed higher β-strand and α-helix content present in 1SlLMW-s. Phylogenetic tree revealed that 1SlLMW-s had close evolutionary relationship with other s-type low molecular weight glutenin subunit (LMW-GSs) genes from different Triticum and Aegilops genomes, which was divergent from LMW-s type gene subfamily at 3.92–5.23 million years ago (MYA). LMW-GSs play a key role in improving breadmaking quality. Abundant expression and specific structural features could contribute to superior gluten quality, including larger and more regular repeated domain, higher proportion of glutamine residues and higher β-strand and α-helix content. This could facilitate the formation of stronger dough structure and superior breadmaking quality. Our work demonstrated that Sl genome had potential glutenin gene resources, and particularly 1SlLMW-s gene could be useful for wheat quality improvement

This article offers supplementary material which is provided at the end of the article.

Keywords: CS-1Sl(1B); LMW-GSs; gluten quality; molecular clone; phylogenetics

Electronic supplementary material. The online version of this article (DOI: 10.1515/biolog-2016-0147) contains supplementary material, which is available to authorized users.

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About the article

#Contributed equally to this work


Received: 2016-03-24

Accepted: 2016-09-28

Published Online: 2016-12-24

Published in Print: 2016-11-01


Citation Information: Biologia, Volume 71, Issue 11, Pages 1212–1222, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.1515/biolog-2016-0147.

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