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Volume 71, Issue 11


Genetic differentiating Aphis fabae and Aphis craccivora (Hemiptera: Sternorranycha: Aphididae) populations in Egypt using mitochondrial COI

Ashraf Helmi
  • Plant Protection Department, Faculty of Agriculture, Ain Shams University, Cairo, 11241, Egypt
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Abdoallah Sharaf
  • Corresponding author
  • Biology Centre ASCR, Institute of Parasitology, Branisovska 31, 37005 Ĉeskě Buděejovice, Czech Republic
  • Genetic Department, Faculty of Agriculture, Ain Shams University, Cairo, 11241, Egypt
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-12-25 | DOI: https://doi.org/10.1515/biolog-2016-0151


Aphis craccivora and Aphis fabae were found to infest broad bean in Egypt. Aphis craccivora was found to be more dominant than A. fabae; A. craccivora was collected from nine locations that represented different agroecosystems in Egypt. At the same time, A. fabae was found in one location only. Single Nucleotide Polymorphisms (SNPs) in mtDNA Cytochrome c oxidase subunit I (COI) were used to differentiate A. fabae and geographically related populations of A. craccivora in Egypt. For this purpose COI primers were designed and registered in GenBank. The primers were successful in detecting genetic variations between both related Aphis species as well as among different geographic populations of A. craccivora. Fourteen SNPs were found to differ among two biotypes of A. craccivora in the Nile Delta and Upper Egypt, probably due to environmental variations in the two different ecosystems.

Keywords: DNA sequencing; Aphididae; COI; biotypes; SNPs; molecular phylogeny


  • Altschul S.F., Gish W., Miller W., Myers E.W. & Lipman D.J. 1990. Basic local alignment search tool. J. Molec. Biol. 215 (3): 403–410. CrossrefGoogle Scholar

  • Ansari A.K., Van Emden H.F. & Singh S.R. 1996. Differential reaction of two biotypes of cowpea aphid, Aphis craccivora(Koch) to cowpea, Vigna unguiculata (L) walp. UNISWA Research Journal of Agriculture. Science and Technology 1: 28–34.Google Scholar

  • Béji B., Bouktila D., Mezghani-Khemakhem M., Bouhachem-Boukhris S., Makni M. & Makni H. 2013. Genetic structure of Aphis fabae Scopoli (Hemiptera, Aphididae) in Tunisia, inferred from RAPD markers. Roman. Agricult. Res. 30: 307–315.Google Scholar

  • Blackman R.L. & Eastop V.F. 2000. Aphids on the World's Crops. An Identification and Information Guide. 2nd ed. John Wiley & Sons Ltd., Chichester, 476 pp. ISBN: 978-0-471-85191-2Google Scholar

  • Brady C.M., Asplen M.K., Desneux N., Heimpel G.E., Hopper K.R., Linnen C.R., Oliver K.M., Wulff J.A. & White J.A. 2014. Worldwide populations of the aphid Aphis craccivora are infected with diverse facultative bacterial symbionts. Microb. Ecol. 67 (1): 195–204. CrossrefGoogle Scholar

  • Coeur D’acier A., Jousselin E., Martin J.F. & Rasplus J.Y. 2007. Phylogeny of the genus Aphis Linnaeus 1758 (Homoptera: Aphididae) inferred from mitochondrial DNA sequences. Mol. Phylogen. Evol. 42 (3): 598–611. CrossrefGoogle Scholar

  • Favret C. 2013. Aphid Species File. Version 5.0/5.0. Available: http://aphid.speciesfile.org (accessed 15.09.2013)

  • Fenton B., Woodford J.A.T. & Malloch G. 1998. Analysis of clonal diversity of the peach-potato aphid, Myzus persicae(Sulzer), in Scotland, UK and evidence for the existence of a predominant clone. Mol. Ecol. 7 (11): 1475–1487. CrossrefGoogle Scholar

  • Foottit R.G., Halbert S.E., Miller G.L., Maw E. & Russell L.M. 2006. Adventive aphids (Hemiptera: Aphididae) of America North of Mexico. Proc. Entomol. Soc. Wash. 108 (3): 583–610.Google Scholar

  • Foottit R.G., Lowery D.T., Maw H.E.L., Smirle M.J. & Lushai G. 2009. Identification, distribution and molecular characterization of the apple aphids Aphis pomi and Aphis spiraecola(Hemiptera: Aphididae: Aphidinae). Can. Entomol. 141 (5): 478–495. CrossrefGoogle Scholar

  • Foottit R.G., Maw H.E.L., von Dohlen C.D. & Herbert P.D.N. 2008. Species identification of aphids (Insecta: Hemiptera: Aphididae) through DNA barcodes. Mol. Ecol. Resour. 8 (6): 1189–1201. .CrossrefGoogle Scholar

  • Gurney T., Elbel R., Ratnapradipa D. & Brossard R. 2000. Introduction to the molecular phylogeny of insects. Chapter 3, pp. 63-79. In: Karcher, S.J. (ed.), Tested Studies for Laboratory Teaching. Vol. 21. Proceedings of the 21st Workshop/Conference of the Association for Biology Laboratory Education (ABLE), June 1-5 1999, 509 pp. ISBN-10: 1890444030, ISBN-13: 9781890444037Google Scholar

  • Heie O.E. 1986. The Aphidoidea (Hemiptera) of Fennoscandia and Denmark. Vol. III. Family Aphididae: subfamily Pte-rocommatinae and tribe Aphidini of subfamily Aphidinae. Fauna Entomol. Scand. 17: 1–314. ISBN-13: 9789004080881Google Scholar

  • Kearse M., Moir R., Wilson A., Stones-Havas S., Cheung M., Sturrock S., Buxton S., Cooper A., Markowitz S., Duran C., Thierer T., Ashton B., Meintjes P. & Drummond A. 2012. Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28 (12): 1647–1649. CrossrefGoogle Scholar

  • Lee W., Kim H., Lim J., Choi H.R., Kim Y., Kim Y.S., Ji J.Y., Foottit R.G. & Lee S. 2011. Barcoding aphids (Hemiptera: Aphididae) of the Korean Peninsula: updating the global data set. Mol. Ecol. Resour. 11 (1): 32–37. CrossrefGoogle Scholar

  • Loxdale H.D. & Lushai G. 1998. Molecular markers in entomology. Bull. Entomol. Res. 88 (6): 577–600. CrossrefGoogle Scholar

  • Messing R.H., Tremblay M.N., Mondor E.B., Foottit R.G. & Pike K.S. 2007. Invasive aphids attack native Hawaiian plants. Biol. Invasions 9 (5): 601–607. CrossrefGoogle Scholar

  • Miller N.J., Birley A.J., Overall A.D.J. & Tatchell G.M. 2003. Population genetic structure of the lettuce root aphid, Pemphigus bursarius (L.), in relation to geographic distance. Geneflow and host plant usage. Heredity 91 (3): 217–223. CrossrefGoogle Scholar

  • Pearson W. & Lipman D. 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85 (8): 2444–2448. PMID: 3162770Google Scholar

  • Ratnasingham S. & Hebert P.D.N. 2007. BOLD: The Barcode of Life Data System (www.barcodinglife.org). Mol. Ecol. Notes 7: 355–364. CrossrefGoogle Scholar

  • Remaudière G. & Remaudièr Catalogue des AphididaeeM. 1997. Catalogue des Aphididae du monde. Homoptera Aphidoidea. Techniques et Pratiques. INRA, Paris, 473 pp.ISBN: 2-7380-0714-7Google Scholar

  • Rogers S.O. & Bendich. 1985. Extraction of DNA from milligram amounts of fresh, herbariurp and mummified plant tissues. Plant Mol. Biol. 5 (2): 69–76. CrossrefGoogle Scholar

  • Rozen S. & Skaletsky H. 1999. Primer3 on the WWW for general users and for biologist programmers, pp. 365-386. In: Misener S. & Krawetz S.A. (eds), Bioinformatics Methods and Protocols, Part 3., Series: Meth. Mol. Biol. Vol. 132. , 490 pp. ISBN: 978-0-89603-732-8CrossrefGoogle Scholar

  • Sambrook J. & Russell D.W. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Vols. 1, 2, 3. Cold Spring Harbor Laboratory Press, New York, 2100 pp. ISBN-10: 0-87969-577-3, ISBN-13: 978-0-87969-577-4Google Scholar

  • Sanger F. & Coulson A.R. 1975. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J. Mol. Biol. 94 (3): 441–448. CrossrefGoogle Scholar

  • Shufran K.A. & Puterka G.J. 2011. DNA barcoding to identify all life stages of holocyclic cereal aphids (Hemiptera: Aphididae) on wheat and other poaceae. Ann. Entomol. Soc. Am. 104 (1): 39–42. CrossrefGoogle Scholar

  • Sloane M.A., Sunnucks P., Wilson A.C.C. & Hales D.F. 2001. Microsatellite isolation, linkage group identification and de-termination of recombination frequency in the peach-potato aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Genet. Res. 77 (3): 251–260. CrossrefGoogle Scholar

  • Smith M.A., Rodriguez J.J., Whitfield J.B., Deans A.R., Janzen D.H., Hallwachs W. & Hebert P.D.N. 2008. Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections. Proc. Natl. Acad. Sci. USA 105 (34): 12359–12364. CrossrefGoogle Scholar

  • Sreejith K. & Sebastian C.D. 2014. Phylogenetic analysis and sequencing of the mitochondrial cytochrome oxidase sub unit I (COI) of White backed plant hopper, Sogatella furcifera (Horvath). Int. Res. J. Pharm. 5 (12): 887–890. CrossrefGoogle Scholar

  • Stamatakis A. 2014. RAxML Version 8: A Tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies. Bioinformatics. 30 (9): 1312–1313. .CrossrefGoogle Scholar

  • Stroyan H.L.G. 1984. Aphids – Pterocommatinae and Aphidinae (Aphidini). Handbooks for the Identification of British Insects Vol. 2, 232 pp. ISBN-10: 0901546623, ISBN-13: 978–0901546623Google Scholar

  • Valenzuela I., Hoffmann A.A., Malipatil M.B., Ridland P.M. & Weeks A.R. 2007. Identification of aphid species (Hemiptera: Aphididae: Aphidinae) using a rapid polymerase chain reaction restriction fragment length polymorphism method based on the cytochrome oxidase subunit I gene. Austral. J. Entomol. 46 (4): 305–312. CrossrefGoogle Scholar

  • Vega F.E., Davis R.E., Barbosa P., Dally E.L., Purcell A.H. & Lee I.M. 1993. Detection of a plant pathogen in a nonvector insect species by the polymerase chain reaction. Phytopathology 83 (6): 621–624. CrossrefGoogle Scholar

  • Wang J.F. & Qiao G.X. 2009. DNA barcoding of genus ToxopteraKoch (Hemiptera: Aphididae): identification and molecular phylogeny inferred from mitochondrial COI sequences. Insect Sci. 16 (6): 475–484. CrossrefGoogle Scholar

  • Xu Z., Chen J. & Cheng D.F.A. 2011. Genetic variation among the geographic population of the grain aphid, Sitobion avenae(Hemiptera: Aphididae) in China inferred from mitochondrial COI gene sequence. J. Integr. Agricult. 10 (7): 1041–1048. CrossrefGoogle Scholar

  • Zhang H.H., Huang X.L., Jiang L.Y., Qiao G.X. & Zheng Z.M. 2010. Subspecies differentiation of Aphis fabae Scopoli (Hemiptera: Aphididae) based on morphological and molecular data. Acta Zootaxonomica Sinica 35 (3): 537–545.Google Scholar

  • Zhang G.X. & Zhong T.S. 1981. Studies on Chinese Aphis craccivora complex with descriptions of two new species and two new subspecies. Sinozoologia 1: 39–43.Google Scholar

  • Zou C., Yang X., Chen X. & Li Y. 2000. Repeat sequence primer-PCR study on DNA polymorphism of geographic populations of cotton aphid, Aphis gossypii, in China. Entomol. Sin. 7 (4): 315–321.Google Scholar

About the article

Received: 2016-04-01

Accepted: 2016-11-18

Published Online: 2016-12-25

Published in Print: 2016-11-01

Citation Information: Biologia, Volume 71, Issue 11, Pages 1266–1273, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.1515/biolog-2016-0151.

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