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Effect of cold treatment on germination of Saxifraga aizoides and S. paniculata: a comparison of central (eastern Alps) and southern populations (northern Apennines)

  • Marco Landi , Silvia Biondini , Antonio Zoccola , Gino Gobbo , Adriano Betti , Carlo Saveri , Patrizio Terlicher , Alessandro Bottacci and Claudia Angiolini
From the journal Biologia

Abstract

The best duration of cold treatment for germination of Saxifraga aizoides and S. paniculata was investigated comparing seeds collected from Alpine and Apennine populations. For S. aizoides seeds of both provenances, the highest germination was obtained after 12 weeks of cold treatment, and Apennine seeds showed the best performance. For S. paniculata, the effect of cold treatment differed greatly in relation to provenance: Alpine seeds showed theirs highest germination after 20 weeks, while Apennine seeds showed their best, albeit low, germination percentage after 8–16 weeks. For both species, a maximum germination plateau was reached 20 days after sowing. Thus, for ex situ conservation and restoration projects in the Apennines, we suppose that S. aizoides seeds should be collected from the location closest to the restoration site and S. paniculata seeds from the Alps. These results also suggest that specific responses to cold treatment exist within the genus Saxifraga in relation to provenance.

Acknowledgements

We acknowledge the staff of the CNBF-Pieve Santo Stefano (Arezzo, Italy) where germinated seeds were planted to raise plants. We thank Giovanni Quilghini and Barbara Rossi for cooperation during the study, OSMER ARPA FVG for climate data, and two anonymous referees for useful comments that improved the manuscript.

References

Aiken S.G., Dallwitz M.J., Consaul L.L., McJannet C.L., Boles R.L., Argus G.W., Gillett J.M., Scott P.J., Elven R., LeBlanc M.C., Gillespie L.J., Brysting A.K., Solstad H. & Harris J.G. 2007. Flora of the Canadian Arctic Archipelago: Descriptions, Illustrations, Identification, and Information Retrieval. NRC Research Press, National Research Council of Canada, Ottawa, Ontario, Canada. http://nature.ca/aaflora/data (accessed on 19 April 2015).Search in Google Scholar

Bewley J.D. & Black M. 1982. Physiology and Biochemistry of Seeds. Vol. 2. Viability, Dormancy, and Environmental Control. Springer-Verlag, Berlin.10.1007/978-3-642-68643-6_2Search in Google Scholar

Cavieres L.A. & Arroyo M.T.K., 2000. Seed germination response to cold stratification period and thermal regime in Phacelia secunda (Hydrophyllaceae). Plant Ecol. 149:1–8.10.1023/A:1009802806674Search in Google Scholar

Chien C.T., Chen S.Y., Chien T.Y., Baskin J.M. & Baskin C.C. 2011. Nondeep simple morphophysiological dormancy in seeds of Ilex maximowicziana from northern (subtropical) and southern (tropical) Taiwan. Ecol. Res. 26:163–171.10.1007/s11284-010-0773-4Search in Google Scholar

Christ H. 1882. Das Pflanzenleben der Schweiz. Schulthess, Zurich.10.5962/bhl.title.9798Search in Google Scholar

Del Vecchio S., Mattana E., Acosta A.T.R. & Bacchetta G. 2012. Seed germination responses to varying environmental conditions and provenances in Crucianella maritima L., a threatened coastal species. C. R. Biologies 335:26–31.10.1016/j.crvi.2011.10.004Search in Google Scholar

Ellis R.H., Hong T.D. & Roberts E.H. 1985. Handbook of seed technology for genebanks. Vol. 1, Principles and Methodology. International Board for Plant Genetic Resources (IBPGR), Roma.Search in Google Scholar

ENSCONET, 2009. European native seed conservation network, Curation Protocols & Recommendations, Royal Botanic Gardens, Kew.Search in Google Scholar

Giménez-Benavides L. & Milla R. 2013. Comparative germination ecology of two altitudinal vicariant Saxifraga species endemic to the north of Spain. Plant Biol. 15:593–600.10.1111/j.1438-8677.2012.00663.xSearch in Google Scholar

Giménez-Benavides L., Escudero A. & Pérez-García F. 2005. Seed germination of high mountain Mediterranean species: altitudinal, interpopulation and interannual variability. Ecol. Res. 20:433–444.10.1007/s11284-005-0059-4Search in Google Scholar

Godefroid S., Piazza C., Rossi G., Buord S., Stevens A.-D., Aguraiuja R., Cowell C., Weekley C.W., Vogg G., Iriondo J.M., Johnson I., Dixon B., Gordon D., Magnanon S., Valentin B., Bjureke K., Koopman R., Vicens M., Virevaire M. & Vanderborght T. 2011. How successful are plant species reintroductions? Biol. Conserv. 144:672–68210.1016/j.biocon.2010.10.003Search in Google Scholar

Gugerli F. 1997. Sexual reproduction in Saxifraga oppositifolia L. and Saxifraga biflora All. (Saxifragaceae) in the Alps. Int. J. Plant Sci. 158:274–281.10.1086/297438Search in Google Scholar

Honˇek A. & Martinková Z. 1999. Seed pre-treatment effects on Echinochloa crus-galli seed dormancy and germination. Biologia 54:423–429.Search in Google Scholar

Ingvarsson P.K. 2001. Restoration of genetic variation lost – the genetic rescue hypothesis. Trends Ecol. Evol. 16:62–63.10.1016/S0169-5347(00)02065-6Search in Google Scholar

Kaplan K. 1995. Saxifragaceae, pp. 130–229. In: Weber H.E. & Hegi G. (eds), Illustrierte Flora von Mitteleuropa II, Blackwell, Berlin.Search in Google Scholar

Korneck D., Schnittler M. & Vollmer I. 1996. Rote Liste der Farn-und Bl¨utenpflanzen (Pteridophyta und Spermatophyta) Deutschlands, pp. 21–187. In: Ludwig G. & Schittler M. (eds), Rote Listen gefährdeter Pflanzen Deutschlands, Schriftenreihe f¨ur Vegetationskunde 28. Bonn.Search in Google Scholar

Landolt E. 1991. Gefährdung der Farn-und Bl¨utenpflanzen in der Schweiz: mit gesamtschweizerischen und regionalen Roten Listen. BUWAL, Bern, 185pp.Search in Google Scholar

Lutz E., Schneller J.J. & Holderegger R. 2000. Understanding population history for conservation purposes: population genetic of Saxifraga aizoides (Saxifragaceae) in the lowlands and lower mountains north of the Alps. Am. J. Bot. 87:583– 590.10.2307/2656602Search in Google Scholar

Maschinski J. & Wright S.J. 2006. Using ecological theory to plan restorations of the endangered beach jacquemontia (Convolvulaceae) in fragmented habitats. J. Nat. Conserv. 14:180–189.10.1016/j.jnc.2006.05.003Search in Google Scholar

McCune B. & Grace J.B. 2002. Analysis of Ecological Communities. MjM Software Design, Gleneden Beach, Oregon, USA, 300 pp.Search in Google Scholar

Meier C. & Holderegger R. 1998. Breeding system, germination, and phenotypic differences among populations of Saxifraga aizoides (Saxifragaceae) at the periphery of its alpine distribution. Nord. J. Bot. 18:681–688.10.1111/j.1756-1051.1998.tb01550.xSearch in Google Scholar

Menges E.S., Guerrant E.O., Hamze S., 2004. What is the effect of seed collection on extinction risk, pp. 305–324. In: Guerrant E.O., Havens K. & Maunder M. (eds), Saving the Pieces: The Value, Limits, and Practice of Off-site Plant Conservation in Support of Wild Diversity, Island Press, Washington.Search in Google Scholar

Meyer S.E., Allen P.S. & Beckstead J. 1997. Seed germination regulation in Bromus tectorum (Poaceae) and its ecological significance. Oikos 78:475–485.10.2307/3545609Search in Google Scholar

Meyer S.E., Nelson D.L. & Carlson S.L. 2004. Ecological genetics of vernalization response in Bromus tectorum L. (Poaceae). Ann. Bot. London 93:653–663.10.1093/aob/mch088Search in Google Scholar PubMed PubMed Central

Meyer S.E. & Pendleton R.L. 2000. Genetic regulation of seed dormancy in Purshia tridentata (Rosaceae). Ann. Bot. London 85:521–529.10.1006/anbo.1999.1099Search in Google Scholar

Pignatti S. 1982. Flora d’Italia, Vol. 1, Edagricole, Bologna.Search in Google Scholar

Pindel Z. & Grucel J. 1982. Optimizing germination conditions of Saxifraga paniculata Mill. and Dianthus glacialis Haenke seeds. Zeszyty Problemowe Postepow Nauk Rolniczych, 491:195–200.Search in Google Scholar

Raffl C., Marcante S. & Erschbamer B. 2007. The role of spontaneous selfing in the pioneer species Saxifraga aizoides.Flora 202:128–132.10.1016/j.flora.2006.05.002Search in Google Scholar

Reisch C. 2008. Glacial history of Saxifraga paniculata (Saxifragaceae): molecular biogeography of a disjunct arctic-alpine species from Europe and North America. Biol. J. Linn. Soc. 93:385–398.10.1111/j.1095-8312.2007.00933.xSearch in Google Scholar

Reisch C., Poschlod P. & Wingender R. 2003. Genetic variation of Saxifraga paniculata Mill. (Saxifragaceae): molecular evidence for glacial relict endemism in central Europe. Biol. J. Linn. Soc. 80:11–21.10.1046/j.1095-8312.2003.00215.xSearch in Google Scholar

Richards C.M. 2000. Inbreeding depression and genetic rescue in a plant metapopulation. Am. Nat. 155:383–394.10.1086/303324Search in Google Scholar PubMed

Schlag R.N. & Erschbamer B. 2000. Germination and establishment of seedlings on a glacier foreland in the Central Alps, Austria. Arctic Antarctic Alpine Res. 32:270–277.10.1080/15230430.2000.12003364Search in Google Scholar

Schwienbacher E. & Erschbamer B. 2001. Longevity of seeds in a glacier foreland of the Central Alps – a burial experiment. Bull. Geobot. Inst. ETH Zurich 68:63–71.Search in Google Scholar

Sokal R.R. & Rohlf F.J. 1995. Biometrics (3-rd Edition). Freeman & Co., New York.Search in Google Scholar

Tutin T.G., Burges N.A., Charter A.O., Edmondson J.R., Hey-wood V.H., Moore D.M., Valentine D.H., Walters S.M. & Webb D.A. 1993. Flora Europea 1, Cambridge University Press, Cambridge.Search in Google Scholar

Received: 2015-7-21
Accepted: 2016-1-11
Published Online: 2016-3-25
Published in Print: 2016-2-1

© 2016 Institute of Botany, Slovak Academy of Sciences

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