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Volume 71, Issue 3


Production of endoglucanase from Trichoderma reesei RUT C30 and its application in deinking of printed office waste paper

Ayman Salih Omer Idris
  • Centre for Biofuels, Biotechnology Division, CSIR – National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram – 695019, India
  • Academy of Scientific and Innovative Research, New Delhi – 110025, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ashok Pandey
  • Centre for Biofuels, Biotechnology Division, CSIR – National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram – 695019, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Rajeev Kumar Sukumaran
  • Corresponding author
  • Centre for Biofuels, Biotechnology Division, CSIR – National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram – 695019, India
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-04-20 | DOI: https://doi.org/10.1515/biolog-2016-0046


Media components were optimized using two-step statistical design of experiments for enhancing endoglucanase/ carboxymethyl cellulase (CMCase) production by Trichoderma reesei RUT C30. A Placket-Burman design identified cellulose concentration and pH as the most significant variables, which influenced the CMCase activity. Central composite design was employed to optimize these selected parameters. The optimal activity was obtained at cellulose concentration 19.7 g/L and pH of 7.2. Under the optimized conditions, CMCase activity was 83.63 ± 1.86 IU/mL and filter paper activity was 2.58 ± 0.2 filter paper units per mL. Enzyme productivity was higher compared to previous reports. The enzyme produced from T. reesei was concentrated and was evaluated for deinking of printed paper, which demonstrated the suitability of the enzyme for this application.

Keywords: cellulase; Trichoderma; endoglucanase; deinking; paper and pulp; response surface


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About the article

Accepted: 2016-03-19

Received: 2015-12-31

Published Online: 2016-04-20

Published in Print: 2016-03-01

Citation Information: Biologia, Volume 71, Issue 3, Pages 265–271, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.1515/biolog-2016-0046.

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