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Biologia

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Volume 71, Issue 4

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Molecular cloning of HMW glutenin subunits from Roegneria kamoji (Poaceae: Triticeae)

Ying-Xia Lei
  • Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
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/ Kuai Yu
  • Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
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/ Yi Wang
  • Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
  • Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
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  • De Gruyter OnlineGoogle Scholar
/ Xing Fan
  • Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
  • Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
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/ Hou-Yang Kang
  • Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
  • Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
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  • De Gruyter OnlineGoogle Scholar
/ Li-Na Sha
  • Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
  • Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
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/ Yong–Hong Zhou
  • Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
  • Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
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/ Hai-Qin Zhang
  • Corresponding author
  • Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
  • Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang 611130, Chengdu, Sichuan, China
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Published Online: 2016-05-15 | DOI: https://doi.org/10.1515/biolog-2016-0055

Abstract

In the present study, The hexaploid species Roegneria kamoji (StStYYHH genomes) in Triticeae produced a single high molecular weight glutenin subunit (HMW-GS) 1St1.2 in its endosperm. 1St1.2 encoded 404 amino acid, which is shorter than previous reported HMW-GSs in Triticeae. The N-terminal domains of 1St1.2 resemble that of the y-type subunits, while their C-terminal domains are similar to the x-type subunits. We discovered many variation types of polypeptides in central repetitive domain. There also have amino acid mutations in the N-terminal domain. The results of phylogenetic analysis suggested that 1St1.2 might be expression from the St genome. Therefore, the results indicated that 1St1.2 is a novel HMW-GS variant or isoform, and the characterization of the subunit can enhance our understanding on the structural differentiation and evolutionary relationships of HMW-GSs in wheat and its wild relatives.

Key words: Roegneria kamoji; HMW-GS; evolution; molecular cloning; Triticeae

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About the article


Received: 2016-01-25

Accepted: 2016-03-24

Published Online: 2016-05-15

Published in Print: 2016-05-01


Citation Information: Biologia, Volume 71, Issue 4, Pages 414–420, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.1515/biolog-2016-0055.

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