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Biologia




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Volume 72, Issue 3

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Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula

Violeta Slavkovska
  • Corresponding author
  • Department of Botany, University of Belgrade, Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia
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/ Branislava Lakušić
  • Department of Botany, University of Belgrade, Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia
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/ Dmitar Lakušić
  • Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Faculty of Biology, Takovska 43 Belgrade, Serbia
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/ Radiša Jančić
  • Department of Botany, University of Belgrade, Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia
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Published Online: 2017-04-21 | DOI: https://doi.org/10.1515/biolog-2017-0029

Abstract

The anatomical structure of the leaf and stem of nine Micromeria species from the Balkan Peninsula was investigated with the aim to establish the adaptive characteristics and traits that could be useful in the identification of species and subgeneric classification. The species included in the anatomical analysis were:Micromeria albanica (Griseb. ex K. Malý) Šilić, M.thymifolia (Scop.) Fritch , M. pulegium (Rochel) Bentham, M. dalmatica Bentham of section Pseudomelissa and M. cristata (Hampe) Griseb., M. kosaninii Šilić, M. juliana (L.) Bentham ex Reichenb., M. croatica (Pers.) Schott M. parviflora (Vis.) Reichenb. of section Micromeria. Variability of 17 quantitative characters of the leaf (thickness, height of adaxial and abaxial epidermis, thickness of the palisade and spongy tissue) and stem (radius, cortex thickness, thickness of the vascular cylinder, phloem, xylem, pith radius) was examined. Multivariate analysis of variance (ANOVA), principal component analysis (PCA), canonical discriminant analysis (CDA) and UPGMA clustering method based on Mahalanobis’ distances were used to determine the variability structure and level of importance of the anatomical differentiation of the investigated taxa. Qualitative characters were also included in the consideration of the anatomical variability, such as leaf shape, leaf margin, position of sclerenchyma fibers in the leaf and stem. The results showed that investigated Micromeria species posses a xeromorphic general structure plan, but each species has achieved distinctive adaptations according to its specific genetic potential. The results have shown a clear difference between the species belonging to the different sections of genus Micromeria, so they have a potential diagnostic and systematic value.

Key words: Micromeria Benth; Pseudomelissa Benth; anatomy; Balkan Peninsula

References

  • Arabaci T., Dirmenci T. & Celep F. 2010. Morphological character analysis in Turkish Micromeria Benth. (Lamiaceae) species with a numerical taxonomic study. Turk. J. Bot. 34: 379–389.Google Scholar

  • Bräuchler C., Meimberg H., Abele T. & Heubl G. 2005. Polyphyly of the genus Micromeria (Lamiaceae): Evidence from cpDNA sequence data. Taxon 54: 639–650.Google Scholar

  • Bräuchler C., Meimberg H. & Heubl G. 2006. New names in Old World Clinopodium – the transfer of the species of Micromeria sect. Pseudomelissa to Clinopodium. Taxon 55: 977–981.Google Scholar

  • Bräuchler C., Ryding O. & Heubl G. 2008. The genus Micromeria (Lamiaceae), a synoptical update. Willdenowia 38: 363–410.Google Scholar

  • Celep F., Kahraman A., Atalay Z. & Doğan M. 2014. Morphology, anatomy, palynology, mericarp and trichome micromorphology of the rediscovered Turkish endemic Salvia quezelii (Lamiaceae) and their taxonomic implications. Plant. Syst. Evol. 300: 1945–1958.Google Scholar

  • Chater O.A. & Guinea E. 1972. Micromeria Bentham, pp. 167– 70. In: Tutin G.T., Heywood H.V., Burges A.N., Moore M.D., Valentine H.D., Walters M.S. & Webb A.D. (eds), Flora Europaea, Vol. 3, Cambridge University Press, London.Google Scholar

  • Dinç M. & Doğu S. 2012. Anatomical and micromorphological studies on Teucrium sect. Isotriodon (Lamiaceae) in Turkey with a taxonomic note. Biologia 67: 663–672.Google Scholar

  • Doroszenko M.A. 1986. Taxonomic studies of Satureja complex (Labiatae). PhD Thesis, University of Edinburgh, Edinburgh.Google Scholar

  • Drew T.B. & Sytsma J.K. 2012. Phylogenetics, biogeography, and staminal evolution in the tribe Mentheae (Lamiaceae). Am. J. Bot. 99: 933–953.Web of ScienceGoogle Scholar

  • Fahn A. & Cutler F.D. 1992. Xerophytes. Gebrüder Borntraeger, Berlin, Stuttgart.Google Scholar

  • Harley R.M., Atkıns S., Budantsev A., Cantıno P.D., Conn B.J., Grayer R., Harley M.M., De Kok .R, Krestovskaja T., Morales R., Paton A.J., Rydıng O. & Upson T. 2004. Labiatae, pp. 167–275. In: Kadereıt J.W. (ed.), The families and genera of vascular plants, Springer, Berlin.Google Scholar

  • Horvat I., Glavač V. & Ellenberg H. 1974. Vegetation Südosteuropas. Gustav Fischer Verlag, Jena, 767 pp.Google Scholar

  • Jäger E.J. & Welk E. 2003. Pflanzengeographische Gliederung Europas, pp. 79 – 86. In: Bohn U. & Neuhäusl R. (eds), Karte der natürlichen Vegetation Europas 1, Landwirtschaftsverlag, Münster.Google Scholar

  • Karousou R., Hanlidou E. & Lazarib D. 2012. Essential oils of Micromeria dalmatica Benth., a Balkan endemic species of section Pseudomelissa. Chem. Biodiv. 9: 2775–283.Google Scholar

  • Kaya A. & Koca F. 2005. Comparative leaf anatomical studies of Acinos species (Labiatae) from Turkey. Nordic J. Bot. 23: 577–588.Google Scholar

  • Koca F. 1996. Morphological and anatomical studies on Micromeria congesta Boiss. et Hausskn. ex Boiss. Turkish J. Bot. 20: 21–29.Google Scholar

  • Koca F. 2002. Morphological and anatomical properties of Micromeria myrtifolia Boiss. et Hohen. Acta Pharm. Turc. 44: 235–242.Google Scholar

  • Kremer D., Stabentheinerb E., Dunkić V., Dragojević-Müller I., Vujić L., Kosalec I., Balliane D., Bogunić F. & Bezić N. 2012. Micromorphological and chemotaxonomical traits of Micromeria croatica (Pers.) Schott. Chem. Biodiv. 9: 755– 768.Google Scholar

  • Kremer D., Dunkić V., Ruščić M., Matevski V., Ballian D., Bogunić, F., Eleftheriadou E., Stešević D., Kosalec I., BezićN.& Stabentheiner E. 2014a. Micromorphological traits and essential oil contents of Micromeria kerneri Murb. and M. juliana (L.) Benth. (Lamiaceae). Phytochemistry 98: 128–136.Google Scholar

  • Kremer D., Dunkić V., Stešević D., Kosalec I., Ballian D., Bogunić F., Bezić N., & Stabentheiner E. 2014b. Micromorphological traits and essential oil of Micromeria longipedunculata Bräuchler (Lamiaceae). Cent. Eur. J. Biol. 9: 559–568.Google Scholar

  • Lakušić B., Lakušić D., Slavkovska V., Stevanović V. & Stevanović B. 2007. Morpho–anatomical differentation of the Balkan endemic species Teucrium arduini L. (Lamiaceaea). Arch. Biol. Sci. 59: 369–381.Google Scholar

  • Lakušić B., Stevanović B., Jančić R. & Lakušić D. 2010. Habitat– related adaptations in morphology and anatomy of Teucrium (Lamiaceae) species from the Balkan peninsula (Serbia and Montenegro). Flora 205: 633–646.Google Scholar

  • Marin M., Jasnić N. & Ascensăo L. 2013. Histochemical, micromorphology and ultrastructural investigation in glandular trichomes of Micromeria thymifolia. Bot. Serb. 37: 49–53.Google Scholar

  • Meusel H. & Jäger E.J. 1992. Vergleichende Chorologie der Zentraleuropäischen Flora 3. Fischer, Jena.Google Scholar

  • Moon H., Hong S., Smets E. & Huysmans S. 2009. Phylogenetic significance of leaf micromorphology and anatomy in the tribe Mentheae (Nepetoideae: Lamiaceae). Bot. J. Linn. Soc. 160: 211–231.Google Scholar

  • Palić I., Ursić–Janković J. & Stojanović G. 2010. Essential oil composition of three Balkan Micromeria species. J. Essent. Oil Res. 22: 40–44.Google Scholar

  • Salmaki Y., Zarre S., Lindqvist S., Heubl G. & Bräuchler C. 2011. Comparative leaf anatomy of Stachys (Lamiaceae: Lamioideae) in Iran with a discussion on its subgeneric classification. Plant. Syst. Evol. 294: 109–125.Google Scholar

  • Satil F. & Kaya A. 2007. Leaf anatomy and hairs of Turkish Satureja L. (Lamiaceae). Acta Biol. Cracov. Ser Bot. 49: 67–76.Google Scholar

  • Satil F., Kaya A. & Dirmenci T. 2011. The taxonomic value of leaf anatomy and trichome morphology of the genus Cyclotrichium (Lamiaceae) in Turkey. Nordic J. Bot. 29: 38–48.Google Scholar

  • Slavkovska V., Couladis M., Bojović S., Tzakou O., Pavlović M., Lakušić B. & Jančić R. 2005. Essential oil and its systematic significance in species of Micromeria Bentham from Serbia and Montenegro. Plant. Syst. Evol. 255: 1–15.Google Scholar

  • Slavkovska V., Zlatković B., Bräuchler C., Stojanović D., Tzakou O. & Couladis M. 2013. Variations of essential oil characteristics of Clinopodium pulegium (Lamiaceae) depending on phenological stage. Bot. Serb. 37: 97–104.Google Scholar

  • Ševarda L.A., Pavlović S., Jančić R. & Kuznjecova A.G. 1979. Uporedna proučavanja ekofizioloških osobina vrsta Micromeria. thymifolia (Scop.) Fritsch i Micromeria dalmatica Bentham). Matica srpska 56: 179–202.Google Scholar

  • Šilić Č. 1979. Monografija rodova Satureja L. Calamintha Miller, Micromeria Bentham, Acinos Miller i Clinopodium L. u flori Jugoslavije. Zemaljski muzej BiH, Sarajevo, pp. 172–262.Google Scholar

  • Zimmerman M.H & Brown L.C. 1974. Trees, structure and function. Springer, Berlin.Google Scholar

About the article

Received: 2016-07-28

Accepted: 2016-11-29

Published Online: 2017-04-21

Published in Print: 2017-03-01


Citation Information: Biologia, Volume 72, Issue 3, Pages 277–291, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.1515/biolog-2017-0029.

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