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Biologia




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Volume 72, Issue 8

Issues

The in vitro effect of poly (I:C) on cell morphology of a metastatic pharyngeal cell line

Tanja Matijevic Glavan
  • Corresponding author
  • Laboratory for Personalized Medicine, Division of Molecular Medicine, Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia
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  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Martina Mikulandra
  • Laboratory for Personalized Medicine, Division of Molecular Medicine, Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia
  • Department of Radiotherapy and Medical Oncology, University Hospital for Tumors, University Hospital Centre Sisters of Mercy, Ilica 197, 10000 Zagreb, Croatia
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2017-08-31 | DOI: https://doi.org/10.1515/biolog-2017-0103

Abstract

Toll-like receptor 3 (TLR3) belongs to a family of TLRs, which are activated by ligands of bacterial and viral origin. Following ligation, TLRs induce different cellular responses including immune response, apoptosis, cell proliferation, cell migration, etc. TLR3 is induced by dsRNA or its analogue poly (I:C). In our previous research, we have noticed the morphological change in Detroit 562 cells after poly (I:C) stimulation resulting in mesenchymal phenotype. Here we have studied the pathways involved in the observed phenomenon by analyzing cell morphometric parameters. We have demonstrated that the observed changed morphology is not a consequence of increased apoptosis. Rho inhibitor also did not abrogate the induced morphological change, however, it induced the formation of short sheet-like sprouts and the combination of Rho inhibitor and poly (I:C) induced lamellipodia-like structures and more filopodia-like thin antennae sprouts. Finally, we have shown that Rac1 activation and epithelial to mesenchymal transition (EMT) markers (vimentin, fibronectin and Snail) are increased after poly (I:C) stimulation. Since we have previously shown increased migration of Detroit 562 cells after poly (I:C) stimulation, we conclude here that this and the morphological change are the result of EMT and Rac1 activation.

Key words: poly (I:C); toll-like receptor 3; morphology; epithelial to mesenchymal transition; migration; Rac1

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About the article

Received: 2017-02-15

Accepted: 2017-08-17

Published Online: 2017-08-31

Published in Print: 2017-08-28


Citation Information: Biologia, Volume 72, Issue 8, Pages 954–960, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.1515/biolog-2017-0103.

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