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Biologia




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Volume 72, Issue 9

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Isolation and expression analyses of KLUH gene in developing seeds and enhanced seed oil in KLUH overexpressing Brassica juncea transgenics

Siddanna Savadi
  • Corresponding author
  • ICAR – Indian Institute of Wheat and Barley Research, Regional station, Flowerdale, Shimla, 171002, India
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/ Deepak Singh Bisht
  • ICAR – National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110012, India
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/ Shripad Ramachandra Bhat
  • ICAR – National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110012, India
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Published Online: 2017-09-30 | DOI: https://doi.org/10.1515/biolog-2017-0123

Abstract

Improving oil yield of Indian mustard (Brassica juncea) is exigent as it is a major oilseed crop of Indian subcontinent, which has severe shortage of vegetable oil production in the world. Some of the regulators of seed development have been shown to improve oil yield in Arabidopsis. Arabidopsis KLUH (AtKLUH), a maternal regulator of seed size, has been shown to control seed oil content. In this study, we identified three homologs of AtKLUH in B. juncea, BjKLUH1, BjKLUH2-1 and BjKLUH2-2. We observed that BjKLUH1 differentially expresses in developing seeds in B. juncea accessions with varying seed size and oil content. Further, analyses for seed oil content in B. juncea transgenics carrying AtKLUH demonstrated an increase in seed oil up to 8.3% compared to wild-type plants. The results of this study suggest that KLUH may have a role in seed development and is a good candidate for engineering seed oil accumulation in B. juncea.

This article offers supplementary material which is provided at the end of the article.

Key words: fatty acid; seed coat; KLUH; gene overexpression

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About the article

Received: 2017-06-03

Accepted: 2017-09-15

Published Online: 2017-09-30

Published in Print: 2017-09-26


Conflict of interest: The authors declare no conflict of interest.


Citation Information: Biologia, Volume 72, Issue 9, Pages 1023–1030, ISSN (Online) 1336-9563, ISSN (Print) 0006-3088, DOI: https://doi.org/10.1515/biolog-2017-0123.

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