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Biomolecular Concepts

Editor-in-Chief: Di Cera, Enrico


Covered by Web of Science (BIOSIS Previews)

PubMed Indexed

CiteScore 2018: 3.35

SCImago Journal Rank (SJR) 2018: 1.475
Source Normalized Impact per Paper (SNIP) 2018: 0.825

ICV 2018: 124.31

Open Access
Online
ISSN
1868-503X
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Volume 2, Issue 1-2

Issues

Embryonic stem cells: protein interaction networks

Patricia Miang-Lon Ng
  • Stem Cell and Developmental Biology, Genome Institute of Singapore, 60 Biopolis Street, 138672 Singapore
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Thomas Lufkin
Published Online: 2011-03-02 | DOI: https://doi.org/10.1515/bmc.2011.008

Abstract

Embryonic stem cells have the ability to differentiate into nearly all cell types. However, the molecular mechanism of its pluripotency is still unclear. Oct3/4, Sox2 and Nanog are important factors of pluripotency. Oct3/4 (hereafter referred to as Oct4), in particular, has been an irreplaceable factor in the induction of pluripotency in adult cells. Proteins interacting with Oct4 and Nanog have been identified via affinity purification and mass spectrometry. These data, together with iterative purifications of interacting proteins allowed a protein interaction network to be constructed. The network currently includes 77 transcription factors, all of which are interconnected in one network. In-depth studies of some of these transcription factors show that they all recruit the NuRD complex. Hence, transcription factor clustering and chromosomal remodeling are key mechanism used by embryonic stem cells. Studies using RNA interference suggest that more pluripotency genes are yet to be discovered via protein-protein interactions. More work is required to complete and curate the embryonic stem cell protein interaction network. Analysis of a saturated protein interaction network by system biology tools can greatly aid in the understanding of the embryonic stem cell pluripotency network.

Keywords: embryonic stem cells; Oct3/4; pluripotency; protein interaction networks

About the article

Published Online: 2011-03-02

Published in Print: 2011-04-01


Citation Information: BioMolecular Concepts, Volume 2, Issue 1-2, Pages 13–25, ISSN (Online) 1868-503X, ISSN (Print) 1868-5021, DOI: https://doi.org/10.1515/bmc.2011.008.

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[1]
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World Journal of Stem Cells, 2019, Volume 11, Number 7, Page 383
[2]
Gregory M. Kelly and Mohamed I. Gatie
Stem Cells International, 2017, Volume 2017, Page 1

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