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Biomolecular Concepts

Editor-in-Chief: Jollès, Pierre / Mansuy, Isabelle

Editorial Board Member: Avila, Jesus / Bonetto, Valentina / Cera, Enrico / Jorgensen, Erik / Jörnvall, Hans / Lagasse, Eric / Norman, Robert / Pinna, Lorenzo / Raghavan, K. Vijay / Venetianer, Pal / Wahli, Walter

6 Issues per year


SCImago Journal Rank (SJR) 2015: 0.519
Source Normalized Impact per Paper (SNIP) 2015: 0.395
Impact per Publication (IPP) 2015: 1.289

Online
ISSN
1868-503X
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Optical tweezers studies of transcription by eukaryotic RNA polymerases

Ana Lisica
  • BIOTEC, Technical University Dresden, Tatzberg 47/49, D-01307 Dresden, Germany; and Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, D-01307 Dresden, Germany
/ Stephan W. Grill
  • BIOTEC, Technical University Dresden, Tatzberg 47/49, D-01307 Dresden, Germany; and Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, D-01307 Dresden, Germany
  • :
Published Online: 2017-02-21 | DOI: https://doi.org/10.1515/bmc-2016-0028

Abstract

Transcription is the first step in the expression of genetic information and it is carried out by large macromolecular enzymes called RNA polymerases. Transcription has been studied for many years and with a myriad of experimental techniques, ranging from bulk studies to high-resolution transcript sequencing. In this review, we emphasise the advantages of using single-molecule techniques, particularly optical tweezers, to study transcription dynamics. We give an overview of the latest results in the single-molecule transcription field, focusing on transcription by eukaryotic RNA polymerases. Finally, we evaluate recent quantitative models that describe the biophysics of RNA polymerase translocation and backtracking dynamics.

Keywords: optical tweezers; RNA polymerases; single-molecule techniques; transcription

aPresent address: London Centre for Nanotechnology, University College London, London WC1H 0AH, UK


Received: 2016-12-01

Accepted: 2017-01-10

Published Online: 2017-02-21

Published in Print: 2017-03-01


Citation Information: Biomolecular Concepts. Volume 8, Issue 1, Pages 1–11, ISSN (Online) 1868-503X, ISSN (Print) 1868-5021, DOI: https://doi.org/10.1515/bmc-2016-0028, February 2017

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