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Merhof, Dorit

Biomedical Engineering / Biomedizinische Technik

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering and the German Society of Biomaterials

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Habibović, Pamela / Haueisen, Jens / Jahnen-Dechent, Wilhelm / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Leonhardt, Steffen / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Boenick, Ulrich / Jaramaz, Branislav / Kraft, Marc / Lenarz, Thomas / Lenthe, Harry / Lo, Benny / Mainardi, Luca / Micera, Silvestro / Penzel, Thomas / Robitzki, Andrea A. / Schaeffter, Tobias / Snedeker, Jess G. / Sörnmo, Leif / Sugano, Nobuhiko / Werner, Jürgen /


IMPACT FACTOR 2018: 1.007
5-year IMPACT FACTOR: 1.390

CiteScore 2018: 1.24

SCImago Journal Rank (SJR) 2018: 0.282
Source Normalized Impact per Paper (SNIP) 2018: 0.831

Online
ISSN
1862-278X
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Volume 49, Issue 9

Issues

Volume 57 (2012)

Wachstumsverhalten humaner mononukleärer Zellen aus dem Knochenmark und Nabelschnurblut auf einem Kollagenträger zur osteogenen Regeneration / Growth Behaviour of Human Mononuclear Cells Derived from Bone Marrow and Cord Blood on a Collagen Carrier for Osteogenic Regeneration

A Wild / M Jäger / S Lensing-Hoehn / A Werner / R Krauspe
Published Online: 2008-03-17 | DOI: https://doi.org/10.1515/BMT.2004.043

Abstract

Es wurde die Biokompatibilität und osteogenetische Potenz eines porcinen Kollagen I/III-Trägers anhand einer humanen Knochenmarkund Nabelschnurblutzellkultur untersucht. Methode: Nach Isolierung mesenchymaler, mononukleärer Zellen aus Nabelschnurblut und Knochenmark aus dem Beckenkamm erfolgte deren Kultivierung in unterschiedlichen Zelldichten auf einer semipermeablen, porcinen Kollagen I/III-Trägermatrix. Nach 14d in vitro erfolgte die kulturelle osteogene Stimulation mit Dexamethason, Ascorbinsäure und beta-Glycerolphosphat (DAG) bis zum 40. Tag und die anschließende semiquantitative immunchemische Auswertung anhand von osteoblastären und Progenitorzellmarkern. Ergebnisse: Bezüglich der minimal erforderlichen lokalen Zelldichte zeigten mesenchymale Progenitorzellen aus dem Nabelschnurblut im Vergleich zu humanen Progenitoren aus dem Knochenmark geringere Toleranzbereiche für das zelluläre Wachstum und die osteogene Differenzierung. Beide Zellkultursysteme zeigen eine dreidimensionales Wachstum und eine Kalzifizierung nach osteogener Stimulation. Schlussfolgerung: Sowohl im humanen Nabelschnurblut als auch im Knochenmark existieren mesenchymale Vorläuferzellen mit osteogener Potenz unter Kultivierung auf einem Kollagen I/III-Biomaterial.

 

We investigated the biocompatibility and osteogenetic potency of a porcine collagen I/III carrier in a human bone marrow and cord blood cell culture system. Methods: Human mesenchymal mononuclear cells were isolated from cord blood and iliac crest bone marrow and cultivated in various cell densities on a semipermeable porcine collagen I/III carrier. After 14 days of in vitro cultivation both cultures were subjected to osteogenic stimulation by dexamethasone, ascorbic acid and beta-glycerol phosphate (DAG) until day 40. Semiquantitative immunochemical evaluation based on osteoblastic and progenitor cell markers was then done. Results: With regard to the minimal local cell density required for growth and osteogenic differentiation, cord blood derived progenitor cells showed lower tolerance in comparison with bone marrow derived cells. For both cell culture systems threedimensional growth and calcification within the collagen fibres were seen after osteogenic stimulation. Conclusion: Human cord blood and bone marrow derived mesenchymal stem cell are capable of differentiating into osteoblasts after incubation with a collagen I/III biomaterial.

Schlüsselwörter: Biokompatibilität; Kollagen; Nabelschnurblut; Knochenmark; Osteoblast

Key words : Biocompatibility; Collagen; Cord blood; Bone marrow; Osteoblasts

About the article

*Korrespondenzanschrift: Prof. Dr. med. Alexander Wild, Orthopädische Universitätsklinik Leipzig, Direktor: Prof. Dr. G. von Salis-Soglio, Semmelweisstrasse 10, 04103 Leipzig, Tel.: 0341/9723200, Fax: 0341/97232009


Published Online: 2008-03-17

Published in Print: 2004-09-01


Citation Information: Biomedizinische Technik/Biomedical Engineering, Volume 49, Issue 9, Pages 227–232, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/BMT.2004.043.

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