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Merhof, Dorit

Biomedical Engineering / Biomedizinische Technik

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering and the German Society of Biomaterials

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Habibović, Pamela / Haueisen, Jens / Jahnen-Dechent, Wilhelm / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Leonhardt, Steffen / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Boenick, Ulrich / Jaramaz, Branislav / Kraft, Marc / Lenarz, Thomas / Lenthe, Harry / Lo, Benny / Mainardi, Luca / Micera, Silvestro / Penzel, Thomas / Robitzki, Andrea A. / Schaeffter, Tobias / Snedeker, Jess G. / Sörnmo, Leif / Sugano, Nobuhiko / Werner, Jürgen /

IMPACT FACTOR 2018: 1.007
5-year IMPACT FACTOR: 1.390

CiteScore 2018: 1.24

SCImago Journal Rank (SJR) 2018: 0.282
Source Normalized Impact per Paper (SNIP) 2018: 0.831

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Volume 57, Issue 1


Volume 57 (2012)

Osteogenic capacity of transgenic flax scaffolds

Tomasz Gredes / Magdalena Wróbel-Kwiatkowska
  • Faculty of Biotechnology, University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland
  • Other articles by this author:
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/ Marzena Dominiak
  • Department of Oral Surgery, Silesian Piast Medical University of Wroclaw, 50-424 Wroclaw, Poland
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/ Tomasz Gedrange
  • Department of Orthodontics, University Clinics, Technical University of Dresden, 01307 Dresden, Germany
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/ Christiane Kunert-Keil
  • Department of Orthodontics, Ernst-Moritz-Arndt University Greifswald, 17475 Greifswald, Germany
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Published Online: 2012-01-19 | DOI: https://doi.org/10.1515/bmt-2011-0035


The modification of flax fibers to create biologically active dressings is of undoubted scientific and practical interest. Flax fibers, derived from transgenic flax expressing three bacterial genes for the synthesis of poly-3-hydroxybutyric acid (PHB), have better mechanical properties than unmodified flax fibers; do not show any inflammation response after subcutaneous insertion; and have a good in vitro and in vivo biocompatibility. The aim of this study was to examine the applicability of composites containing flax fibers of genetically modified (M50) or non-modified (wt-Nike) flax within a polylactide (PLA) matrix for bone regeneration. For this, the mRNA expression of genes coding for growth factors (insulin-like growth factor IGF1, IGF2, vascular endothelial growth factor), for osteogenic differentiation (alkaline phosphatase, osteocalcin, Runx2, Phex, type 1 and type 2 collagen), and for bone resorption markers [matrix metalloproteinase 8 (MMP8), acid phosphatase type 5] were analyzed using quantitative real-time polymerase chain reaction. We found a significant elevated mRNA expression of IGF1 with PLA and PLA-wt-Nike composites. The mRNA amount of MMP8 and osteocalcin was significantly decreased in all biocomposite-treated cranial tissue samples compared to controls, whereas the expression of all other tested transcripts did not show any differences. It is assumed that both flax composites are able to stimulate bone regeneration, but composites from transgenic flax plants producing PHB showed faster bone regeneration than composites of non-transgenic flax plants. The application of these linen membranes for bone tissue engineering should be proved in further studies.

Keywords: biocomposite; bone regeneration; quantitative RT-PCR; transgenic flax fibers

About the article

Corresponding author: Dr. Tomasz Gredes, Department of Orthodontics, Ernst-Moritz-Arndt University Greifswald, Rotgerberstr. 8, D-17475 Greifswald, Germany Phone: +49-3834-867110 Fax: +49-3834-867113

Received: 2011-07-22

Accepted: 2011-11-28

Published Online: 2012-01-19

Published in Print: 2012-02-01

Citation Information: Biomedizinische Technik/Biomedical Engineering, Volume 57, Issue 1, Pages 53–58, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/bmt-2011-0035.

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