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Biomedical Engineering / Biomedizinische Technik

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering and the German Society of Biomaterials

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Haueisen, Jens / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Lenarz, Thomas / Leonhardt, Steffen / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Boenick, Ulrich / Jaramaz, Branislav / Kraft, Marc / Lenthe, Harry / Lo, Benny / Mainardi, Luca / Micera, Silvestro / Penzel, Thomas / Robitzki, Andrea A. / Schaeffter, Tobias / Snedeker, Jess G. / Sörnmo, Leif / Sugano, Nobuhiko / Werner, Jürgen /

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Volume 58, Issue 2 (Apr 2013)


Volume 57 (2012)

Simultaneous assessment of autonomic nervous and vascular endothelial functions in a rat model

Hsien-Tsai Wu / Cyuan-Cin Liu
  • Corresponding author
  • Department of Electrical Engineering, National Dong Hwa University, Hualien, Taiwan
  • Equal contribution to the corresponding author.
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Cheuk-Kwan Sun / An-Bang Liu
  • Department of Neurology, Buddhist Tzu Chi General Hospital and Buddhist Tzu Chi University, Hualien, Taiwan
  • Equal contribution to the corresponding author.
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ching-Shuen Chen / Chun-Cheng Yang / Chien-Hsing Wang
Published Online: 2013-02-14 | DOI: https://doi.org/10.1515/bmt-2012-0056


Despite the importance of translational medicine in cardiovascular research, rat vascular endothelial function and sympathetic activity assessment remains challenging. This study consisted of two parts: part A examined the consistency of reactive hyperemia-elicited dilatation index (DI) before (DIOriginal) and after (DIEEMD) ensemble empirical mode decomposition (EEMD) using Wistar-Kyoto (WKY) rats (n=7). Part B compared the endothelial function and heart rate variability of normal WKY rats (group 1, n=9) with those of spontaneously hypertensive rats (group 2, n=9) at two time points of development. The SD1/SD2 ratio (SSR) was obtained from Poincaré plot analysis to evaluate sympathetic activities. Using a Bland-Altman plot, part A of the study demonstrated a lower agreement of DIOriginal than that of DIEEMD. In part B, despite no significant difference in DIEEMD between the two groups at age 10 weeks, DIEEMD was higher in group 1 (2.25±0.63 vs. 1.43±0.41 for groups 1 and 2, respectively, p=0.004) at 20 weeks. Although no notable change in SSR existed in group 1 between the two time points, a significant difference existed in group 2 (p<0.001). In conclusion, using algorithms of Poincaré plot analysis and EEMD, the impact of age and hypertension on rat vascular endothelial function and heart rate variability can be reliably assessed.

Keywords: autonomic function; endothelial function; hypertension; Poincaré plot analysis; reactive hyperemia


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About the article

Corresponding author: Cyuan-Cin Liu, Department of Electrical Engineering, National Dong Hwa University, No. 1, Sec. 2, Da Hsueh Road, Shoufeng, Hualien 97401, Taiwan, R.O.C., Phone: +886-3-8634098, Fax: +886-3-8634060

Received: 2012-08-09

Accepted: 2013-01-15

Published Online: 2013-02-14

Published in Print: 2013-04-01

Citation Information: Biomedizinische Technik/Biomedical Engineering, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/bmt-2012-0056.

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