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Biomedical Engineering / Biomedizinische Technik

Joint Journal of the German Society for Biomedical Engineering in VDE and the Austrian and Swiss Societies for Biomedical Engineering and the German Society of Biomaterials

Editor-in-Chief: Dössel, Olaf

Editorial Board: Augat, Peter / Haueisen, Jens / Jockenhoevel, Stefan / Knaup-Gregori, Petra / Lenarz, Thomas / Leonhardt, Steffen / Plank, Gernot / Radermacher, Klaus M. / Schkommodau, Erik / Stieglitz, Thomas / Boenick, Ulrich / Jaramaz, Branislav / Kraft, Marc / Lenthe, Harry / Lo, Benny / Mainardi, Luca / Micera, Silvestro / Penzel, Thomas / Robitzki, Andrea A. / Schaeffter, Tobias / Snedeker, Jess G. / Sörnmo, Leif / Sugano, Nobuhiko / Werner, Jürgen /

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Volume 59, Issue 2 (Apr 2014)

Issues

Volume 57 (2012)

Nondestructive monitoring of tissue-engineered constructs

Julia Frese
  • Department of Tissue Engineering and Textile Implants, Institute of Applied Medical Engineering, Helmholtz Institute of Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany
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/ Agnieszka Morgenroth
  • Department of Nuclear Medicine, University Hospital, RWTH Aachen University, 52074 Aachen, Germany
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/ Marianne E. Mertens
  • Department of Experimental Molecular Imaging, Helmholtz Institute of Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany
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/ Sabine Koch
  • Department of Tissue Engineering and Textile Implants, Institute of Applied Medical Engineering, Helmholtz Institute of Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany
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/ Lisanne Rongen
  • Department of Tissue Engineering and Textile Implants, Institute of Applied Medical Engineering, Helmholtz Institute of Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany
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/ Andreas T.J. Vogg
  • Department of Nuclear Medicine, University Hospital, RWTH Aachen University, 52074 Aachen, Germany
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/ Boris D. Zlatopolskiy
  • Department of Nuclear Medicine, University Hospital, RWTH Aachen University, 52074 Aachen, Germany
  • Max Planck Institute for Neurological Research, 50931 Cologne, Germany
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/ Bernd Neumaier / Valentine N. Gesche
  • Department of Tissue Engineering and Textile Implants, Institut für Textiltechnik, RWTH Aachen University, 52074 Aachen, Germany
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/ Twan Lammers
  • Department of Experimental Molecular Imaging, Helmholtz Institute of Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany
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/ Thomas Schmitz-Rode
  • Department of Tissue Engineering and Textile Implants, Institute of Applied Medical Engineering, Helmholtz Institute of Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany
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/ Petra Mela
  • Department of Tissue Engineering and Textile Implants, Institute of Applied Medical Engineering, Helmholtz Institute of Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany
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/ Stefan Jockenhoevel
  • Department of Tissue Engineering and Textile Implants, Institute of Applied Medical Engineering, Helmholtz Institute of Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany
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/ Felix M. Mottaghy
  • Department of Nuclear Medicine, University Hospital, RWTH Aachen University, 52074 Aachen, Germany
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/ Fabian Kiessling
  • Corresponding author
  • Department of Experimental Molecular Imaging, Helmholtz Institute of Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany
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Published Online: 2013-09-09 | DOI: https://doi.org/10.1515/bmt-2013-0029

Abstract

Tissue engineering as a multidisciplinary field enables the development of living substitutes to replace, maintain, or restore diseased tissue and organs. Since the term was introduced in medicine in 1987, tissue engineering strategies have experienced significant progress. However, up to now, only a few substitutes were able to overcome the gap from bench to bedside and have been successfully approved for clinical use. Substantial donor variability makes it difficult to predict the quality of tissue-engineered constructs. It is essential to collect sufficient data to ensure that poor or immature constructs are not implanted into patients. The fulfillment of certain quality requirements, such as mechanical and structural properties, is crucial for a successful implantation. There is a clear need for new nondestructive and real-time online monitoring and evaluation methods for tissue-engineered constructs, which are applicable on the biomaterial, tissue, cellular, and subcellular levels. This paper reviews current established nondestructive techniques for implant monitoring including biochemical methods and noninvasive imaging.

Keywords: extracellular matrix; imaging techniques; nondestructive analysis; quality management; tissue engineering

References

  • [1]

    Achenbach S, Moselewski F, Ropers D, et al. Detection of calcified and noncalcified coronary atherosclerotic plaque by contrast-enhanced, submillimeter multidetector spiral computed tomography: a segment-based comparison with intravascular ultrasound. Circulation 2004; 109: 14–17.PubMedGoogle Scholar

  • [2]

    Ansar MM, Esfandiariy E, Mardani M, Hashemibeni B, Zarkesh-Esfahani SH, Hatef M, Kabiri A. A comparative study of aggrecan synthesis between natural articular chondrocytes and differentiated chondrocytes from adipose derived stem cells in 3D culture. Adv Biomed Res 2012; 1: 24–31.Google Scholar

  • [3]

    Arima V, Pascali G, Lade O, et al. Radiochemistry on chip: towards dose-on-demand synthesis of PET radiopharmaceuticals. Lab Chip 2013; 13: 2328–2336.PubMedCrossrefGoogle Scholar

  • [4]

    Barrett JA, Coleman RE, Goldsmith SJ, et al. First-in-man evaluation of 2 high-affinity PSMA-avid small molecules for imaging prostate cancer. J Nucl Med 2013; 54: 380–387.CrossrefPubMedGoogle Scholar

  • [5]

    Beckers C, Jeukens X, Ribbens C, et al. 18F-FDG PET imaging of rheumatoid knee synovitis correlates with dynamic magnetic resonance and sonographic assessments as well as with the serum level of metalloproteinase-3. Eur J Nucl Med Mol Imaging 2006; 33: 275–280.CrossrefGoogle Scholar

  • [6]

    Boerboom RA, Krahn KN, Megens RT, van Zandvoort MA, Merkx M, Bouten CV. High resolution imaging of collagen organisation and synthesis using a versatile collagen specific probe. J Struct Biol 2007; 159: 392–399.PubMedCrossrefGoogle Scholar

  • [7]

    Bosman FT, Tamenkovic I. Functional structure and composition of the extracellular matrix. J Pathol 2003; 200: 423–428.CrossrefPubMedGoogle Scholar

  • [8]

    Brauchle E, Schenke-Layland K. Raman spectroscopy in biomedicine – non-invasive in vitro analysis of cells and extracellular matrix components in tissues. Biotechnol J 2013; 8: 288–297.CrossrefGoogle Scholar

  • [9]

    Cai W, Chen K, Mohamedali KA, et al. PET of vascular endothelial growth factor receptor expression. J Nucl Med 2006; 47: 2048–2056.PubMedGoogle Scholar

  • [10]

    Charrier-Hisamuddin L, Laboisse CL, Merlin D. ADAM-15: a metalloprotease that mediates inflammation. FASEB J 2008; 22: 641–653.Google Scholar

  • [11]

    Cheng HL, Loai Y, Farhat WA. Monitoring tissue development in acellular matrix-based regeneration for bladder tissue engineering: multiexponential diffusion and T2* for improved specificity. NMR Biomed 2012; 25: 418–426.Google Scholar

  • [12]

    Cho SY, Gage KL, Mease RC, et al. Biodistribution, tumor detection, and radiation dosimetry of 18F-DCFBC, a low-molecular-weight inhibitor of prostate-specific membrane antigen, in patients with metastatic prostate cancer. J Nucl Med 2012; 53: 1883–1891.CrossrefGoogle Scholar

  • [13]

    Crofford LJ. COX-1 and COX-2 tissue expression: implications and predictions. J Rheumatol Suppl 1997; 49: 15–19.Google Scholar

  • [14]

    Danhier F, Le Breton A, Preat V. RGD-based strategies to target alpha(v) beta(3) integrin in cancer therapy and diagnosis. Mol Pharm 2012; 9: 2961–2973.PubMedCrossrefGoogle Scholar

  • [15]

    Demartis S, Tarli L, Borsi L, Zardi L, Neri D. Selective targeting of tumour neovasculature by a radiohalogenated human antibody fragment specific for the ED-B domain of fibronectin. Eur J Nucl Med 2001; 28: 534–539.PubMedCrossrefGoogle Scholar

  • [16]

    Ferrara N. Vascular endothelial growth factor: basic science and clinical progress. Endocr Rev 2004; 25: 581–611.CrossrefPubMedGoogle Scholar

  • [17]

    Fite BZ, Decaris M, Sun Y, et al. Noninvasive multimodal evaluation of bioengineered cartilage constructs combining time-resolved fluorescence and ultrasound imaging. Tissue Eng Part C Methods 2011; 17: 495–504.CrossrefGoogle Scholar

  • [18]

    Flanagan TC, Pandit A. Living artificial heart valve alternatives: a review. Eur Cell Mater 2003; 6: 25–48.Google Scholar

  • [19]

    Flanagan TC, Sachweh JS, Frese J, et al. In vivo remodelling and structural characterisation of fibrin-based tissue-engineered heart valves in the adult sheep model. Tissue Eng Part A 2009; 15: 2965–2976.CrossrefGoogle Scholar

  • [20]

    Frese J, Hrdlicka L, Mertens ME, et al. Non-invasive imaging of tissue-engineered vascular endothelium with iron oxide nanoparticles. Biomed Technol Berl 2012; 57 (Suppl 1): 1065–1067.Google Scholar

  • [21]

    Frese J, Schuster P, Mertens ME, et al. Generation and imaging of patient customized implants. Biomed Technol (Berl). 2012; 57 (Suppl 1): 87–89.Google Scholar

  • [22]

    Furumoto S, Takashima K, Kubota K, Ido T, Iwata R, Fukuda H. Tumor detection using 18F-labeled matrix metalloproteinase-2 inhibitor. Nucl Med Biol 2003; 30: 119–125.CrossrefGoogle Scholar

  • [23]

    Gordon IO, Tretiakova MS, Noffsinger AE, Hart J, Reuter VE, Al-Ahmadie HA. Prostate-specific membrane antigen expression in regeneration and repair. Mod Pathol 2008; 21: 1421–1427.PubMedCrossrefGoogle Scholar

  • [24]

    Gremse F, Grouls C, Palmowski M, et al. Virtual elastic sphere processing enables reproducible quantification of vessel stenosis at CT and MR angiography. Radiology 2011; 260: 709–717.CrossrefGoogle Scholar

  • [25]

    Grizzle WE. Special Symposium: fixation and tissue processing models. Biotechnol Histochem 2009; 84: 185–193.Google Scholar

  • [26]

    Hagenmüller H, Hofmann S, Kohler T, et al. Non-invasive time-lapsed monitoring and quantification of engineered bone-like tissue. Ann Biomed Eng 2007; 35: 1657–1667.PubMedCrossrefGoogle Scholar

  • [27]

    Haller N, Hollweck T, Thierfelder N, et al. Noninvasive analysis of synthetic and decellularized scaffolds for heart valve tissue engineering. ASAIO J 2013; 59: 169–177.PubMedCrossrefGoogle Scholar

  • [28]

    Herzog H. In vivo functional imaging with SPECT and PET. Radiochim Acta 2001; 89: 203–214.Google Scholar

  • [29]

    Jayapaul J, Hodenius M, Arns S, et al. FMN-coated fluorescent iron oxide nanoparticles for RCP-mediated targeting and labeling of metabolically active cancer and endothelial cells. Biomaterials 2011; 32: 5863–5871.PubMedCrossrefGoogle Scholar

  • [30]

    Kalliokoski T, Simell O, Haaparanta M, et al. An autoradiographic study of [18F]FDG uptake to islets of Langerhans in NOD mouse. Diabetes Res Clin Pract 2005; 70: 217–224.CrossrefGoogle Scholar

  • [31]

    Keidar Z, Engel A, Hoffman A, Israel O, Nitecki S. Prosthetic vascular graft infection: the role of 18F-FDG PET/CT. J Nucl Med 2007; 48: 1230–1236.CrossrefGoogle Scholar

  • [32]

    Koch S, Flanagan TC, Sachweh JS, et al. Fibrin-polylactide-based tissue-engineered vascular graft in the arterial circulation. Biomaterials 2010; 31: 4731–4739.CrossrefPubMedGoogle Scholar

  • [33]

    König K, Schenke-Layland K, Riemann I, Stock UA. Multiphoton autofluorescence imaging of intratissue elastic fibers. Biomaterials 2005; 26: 495–500.CrossrefGoogle Scholar

  • [34]

    Krahn KN, Bouten CV, van Tuijl S, van Zandvoort MA, Merkx M. Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture. Anal Biochem 2006; 350: 177–185.PubMedCrossrefGoogle Scholar

  • [35]

    Krämer NA, Donker HC, Otto J, et al. A concept for magnetic resonance visualization of surgical textile implants. Invest Radiol 2010; 45: 477–483.CrossrefPubMedGoogle Scholar

  • [36]

    Kreitz S, Dohmen G, Hasken S, Schmitz-Rode T, Mela P, Jockenhoevel S. Nondestructive method to evaluate the collagen content of fibrin-based tissue engineered structures via ultrasound. Tissue Eng Part C Methods 2011; 17: 1021–1026.CrossrefGoogle Scholar

  • [37]

    Lees JG, Lim SA, Croll T, et al. Transplantation of 3D scaffolds seeded with human embryonic stem cells: biological features of surrogate tissue and teratoma-forming potential. Regen Med 2007; 2: 289–300.CrossrefGoogle Scholar

  • [38]

    Libby P, Li H. Vascular cell adhesion molecule-1 and smooth muscle cell activation during atherogenesis. J Clin Invest 1993; 92: 538–539.CrossrefGoogle Scholar

  • [39]

    McAfee JG, Thakur ML. Survey of radioactive agents for in vitro labeling of phagocytic leukocytes. I. Soluble agents. J Nucl Med 1976; 17: 480–487.PubMedGoogle Scholar

  • [40]

    Mees G, Dierckx R, Mertens K, et al. 99mTc-labeled tricarbonyl his-CNA35 as an imaging agent for the detection of tumor vasculature. J Nucl Med 2012; 53: 464–471.CrossrefGoogle Scholar

  • [41]

    Mertens, M.E. et al. Iron oxide-labeled collagen scaffolds for non-invasive MR imaging in tissue engineering. Adv Funct Mater 2013; in press.Google Scholar

  • [42]

    Meyer U. The history of tissue engineering and regenerative medicine in perspective. In: Meyer U, Meyer TH, Handschel J, Wiesmann HP, editors. Fundamentals of tissue engineering and regenerative medicine. Berlin Heidelberg: Springer 2009: 5–12.Google Scholar

  • [43]

    Miyata S, Numano T, Homma K, Tateishi T, Ushida T. Feasibility of noninvasive evaluation of biophysical properties of tissue-engineered cartilage by using quantitative MRI J Biomech 2007; 40: 2990–2998.CrossrefPubMedGoogle Scholar

  • [44]

    Nahrendorf M, Keliher E, Panizzi P, et al. 18F-4V for PET-CT imaging of VCAM-1 expression in atherosclerosis. JACC Cardiovasc Imaging 2009; 2: 1213–1222.Google Scholar

  • [45]

    Nakamura A, Dohi Y, Akahane M, et al. Osteocalcin secretion as an early marker of in vitro osteogenic differentiation of rat mesenchymal stem cells. Tissue Eng Part C 2009; 15: 169–180.CrossrefGoogle Scholar

  • [46]

    Nelson GN, Roh JD, Mirensky TL, et al. Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo. Faseb J 2008; 22: 3888–3895.PubMedCrossrefGoogle Scholar

  • [47]

    Papos M, Nagy F, Lang J, Csernay L. Leukocyte scintigraphy to assess disease activity in inflammatory bowel disease. J Nucl Med 1996; 37: 400.PubMedGoogle Scholar

  • [48]

    Pascali G, Watts P, Salvadori PA. Microfluidics in radiopharmaceutical chemistry. Nucl Med Biol 2013; 40: 776–787.CrossrefPubMedGoogle Scholar

  • [49]

    Perea H, Aigner J, Heverhagen JT, Hopfner U, Wintermantel E. Vascular tissue engineering with magnetic nanoparticles: seeing deeper. J Tissue Eng Regen Med 2007; 1: 318–321.CrossrefPubMedGoogle Scholar

  • [50]

    Popp JR, Robert JJ, Gallagher DV, Anseth KS, Bryan SJ, Quinn TP. An instrumented bioreactor for mechanical stimulation and real-time, nondestructive evaluation of engineered cartilage tissue. J Med Devices 2012; 6: 021006-1–021006-7.CrossrefGoogle Scholar

  • [51]

    Potter K, Sweet DE, Anderson P, et al. Non-destructive studies of tissue-engineered phalanges by magnetic resonance microscopy and X-ray microtomography. Bone 2006; 38: 350–358.CrossrefPubMedGoogle Scholar

  • [52]

    Rice MA, Waters KR, Anseth KS. Ultrasound monitoring of cartilaginous matrix evolution in degradable PEG hydrogels. Acta Biomater 2009; 5: 152–161.PubMedCrossrefGoogle Scholar

  • [53]

    Russel AJ, Bertram T. Moving into the clinic. In: Lanza R, Langer R, Vacanti J, editors. Principles of tissue engineering, 3rd edition. Burlington, San Diego, London: Elsevier, Inc. 2007: 15–32.Google Scholar

  • [54]

    Santimaria M, Moscatelli G, Viale GL, et al. Immunoscintigraphic detection of the ED-B domain of fibronectin, a marker of angiogenesis, in patients with cancer. Clin Cancer Res 2003; 9: 571–579.PubMedGoogle Scholar

  • [55]

    Schenke-Layland K, Riemann I, Damour O, Stock UA, König K. Two-photon microscopes and in vivo multiphoton tomographs – powerful diagnostic tools for tissue engineering and drug delivery. Adv Drug Deliv Rev 2006; 58: 878–896.CrossrefGoogle Scholar

  • [56]

    Schenke-Layland K, Riemann I, Stock UA, König K. Imaging of cardiovascular structures using near-infrared femtosecond multiphoton laser scanning microscopy. J Biomed Opt 2005; 10: 024017.CrossrefGoogle Scholar

  • [57]

    Seabold JE, Nepola JV, Conrad GR, et al. Detection of osteomyelitis at fracture nonunion sites: comparison of two scintigraphic methods. AJR Am J Roentgenol 1989; 152: 1021–1027.CrossrefPubMedGoogle Scholar

  • [58]

    Song G, Zhao X, Xu J, Song H. Increased expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in rat cardiac allografts. Transplant Proc 2008; 40: 2720–2723.CrossrefGoogle Scholar

  • [59]

    Spacek M, Belohlavek O, Votrubova J, Sebesta P, Stadler P. Diagnostics of "non-acute" vascular prosthesis infection using 18F-FDG PET/CT: our experience with 96 prostheses. Eur J Nucl Med Mol Imaging 2009; 36: 850–858.CrossrefGoogle Scholar

  • [60]

    Tuan HS, Hutmacher DW. Application of micro CT and computation modeling in bone tissue engineering. Comput Aided Des 2005; 37: 1151–1161.CrossrefGoogle Scholar

  • [61]

    Tuemen M, Nguyen DV, Raffius J, et al. Non-destructive analysis of extracellular matrix development in cardiovascular tissue-engineered constructs. Ann Biomed Eng 2013; 41: 883–893.PubMedCrossrefGoogle Scholar

  • [62]

    Uddin MJ, Crews BC, Blobaum AL, et al. Selective visualization of cyclooxygenase-2 in inflammation and cancer by targeted fluorescent imaging agents. Cancer Res 2010; 70: 3618–3627.Google Scholar

  • [63]

    Uddin MJ, Crews BC, Ghebreselasie K, Tantawy MN, Marnett LJ. [I]-Celecoxib Analogues as SPECT Tracers of Cyclooxygenase-2 in Inflammation. ACS Med Chem Lett 2011; 2: 160–164.Google Scholar

  • [64]

    Uddin MJ, Crews BC, Ghebreselasie K, et al. Fluorinated COX-2 inhibitors as agents in PET imaging of inflammation and cancer. Cancer Prev Res (Phila) 2011; 4: 1536–1545.Google Scholar

  • [65]

    van Hinsbergh VW, Collen A, Koolwijk P. Role of fibrin matrix in angiogenesis. Ann NY Acad Sci 2001; 936: 426–437.Google Scholar

  • [66]

    Wang M, Radjenovic A, Stapleton TW, et al. A novel and non-destructive method to examine meniscus architecture using 9.4 Tesla MRI. Osteoarthr Cartil 2010; 18: 1417–1420.CrossrefGoogle Scholar

  • [67]

    Weinbaum JS, Qi J, Tranquillo RT. Monitoring collagen transcription by vascular smooth muscle cells in fibrin-based tissue constructs. Tissue Eng C Methods 2010; 16: 459–467.CrossrefGoogle Scholar

  • [68]

    Wells PNT. Ultrasonics in medicine and biology. Phys Med Biol 1977; 22: 629–669.CrossrefPubMedGoogle Scholar

  • [69]

    Xu Y, Rivas JM, Brown EL, Liang X, Hook M. Virulence potential of the staphylococcal adhesin CNA in experimental arthritis is determined by its affinity for collagen. J Infect Dis 2004; 189: 2323–2333.CrossrefPubMedGoogle Scholar

  • [70]

    Yang W, Zhang Y, Fu Z, Sun X, Mu D, Yu J. Imaging proliferation of (1)(8)F-FLT PET/CT correlated with the expression of microvessel density of tumour tissue in non-small-cell lung cancer. Eur J Nucl Med Mol Imaging 2012; 39: 1289–1296.Google Scholar

  • [71]

    Zanzonico P. Principles of nuclear medicine imaging: planar, SPECT, PET, multi-modality, and autoradiography systems. Radiat Res 2012; 177: 349–364.CrossrefGoogle Scholar

  • [72]

    Zhang J, Razavian M, Tavakoli S, et al. Molecular imaging of vascular endothelial growth factor receptors in graft arteriosclerosis. Arterioscler Thromb Vasc Biol 2012; 32: 1849–1855.CrossrefGoogle Scholar

About the article

Corresponding author: Fabian Kiessling, Department of Experimental Molecular Imaging, Helmholtz Institute of Biomedical Engineering, RWTH Aachen University, Pauwelsstr. 30, 52074 Aachen, Germany, Phone: +49 241 80 80116, Fax: +49 241 80 33 80116, E-mail:

aThese authors contributed equally to the publication and, therefore, are considered as first authors.


Received: 2013-05-29

Accepted: 2013-08-13

Published Online: 2013-09-09

Published in Print: 2014-04-01


Citation Information: Biomedical Engineering / Biomedizinische Technik, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/bmt-2013-0029.

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