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Biomedical Engineering / Biomedizinische Technik

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Volume 59, Issue 4 (Aug 2014)

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Volume 57 (2012)

Application of floating silicon-based linear multielectrode arrays for acute recording of single neuron activity in awake behaving monkeys

Luca Bonini
  • Corresponding author
  • Italian Institute of Technology (IIT), Brain Center for Social and Motor Cognition, Via Volturno 39, 40125 Parma, Italy
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Monica Maranesi
  • Italian Institute of Technology (IIT), Brain Center for Social and Motor Cognition, Via Volturno 39, 40125 Parma, Italy
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Alessandro Livi / Stefania Bruni / Leonardo Fogassi / Tobias Holzhammer
  • Department of Microsystems Engineering (IMTEK), Microsystem Materials Laboratory, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Oliver Paul
  • Department of Microsystems Engineering (IMTEK), Microsystem Materials Laboratory, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Patrick Ruther
  • Department of Microsystems Engineering (IMTEK), Microsystem Materials Laboratory, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2013-10-26 | DOI: https://doi.org/10.1515/bmt-2012-0099

Abstract

One of the fundamental challenges in behavioral neurophysiology in awake animals is the steady recording of action potentials of many single neurons for as long as possible. Here, we present single neuron data obtained during acute recordings mainly from premotor cortices of three macaque monkeys using a silicon-based linear multielectrode array. The most important aspect of these probes, compared with similar models commercially available, is that, once inserted into the brain using a dedicated insertion device providing an intermediate probe fixation by means of vacuum, they can be released and left floating in the brain. On the basis of our data, these features appear to provide (i) optimal physiological conditions for extracellular recordings, (ii) good or even excellent signal-to-noise ratio depending on the recorded brain area and cortical layer, and (iii) extreme stability of the signal over relatively long periods. The quality of the recorded signal did not change significantly after several penetrations into the same restricted cortical sector, suggesting limited tissue damage due to probe insertion. These results indicate that these probes offer several advantages for acute neurophysiological experiments in awake monkeys, and suggest the possibility to employ them for semichronic or even chronic studies.

Keywords: cerebral cortex; electrophysiology; macaque; recording stability; single unit

References

  • [1]

    Azemi E, Stauffer WR, Gostock MS, Lagenaur CF, Cui XT. Surface immobilization of neural adhesion molecule L1 for improving the biocompatibility of chronic neural probes: in vitro characterization. Acta Biomater 2008; 4: 1208–1217.Web of ScienceCrossrefPubMedGoogle Scholar

  • [2]

    Belmalih A, Borra E, Contini M, Gerbella M, Rozzi S, Luppino G. Multimodal architectonic subdivision of the rostral part (area F5) of the macaque ventral premotor cortex. J Comp Neurol 2009; 512: 183–217.Web of ScienceGoogle Scholar

  • [3]

    Bonini L, Rozzi S, Serventi FU, Simone L, Ferrari PF, Fogassi L. Ventral premotor and inferior parietal cortices make distinct contribution to action organization and intention understanding. Cereb Cortex 2010; 20: 1372–1385.CrossrefPubMedWeb of ScienceGoogle Scholar

  • [4]

    Bonini L, Maranesi M, Caselli L, et al. Functional characterization of monkey ventral premotor grasping neurons classes during execution and observation of a visuo-motor task. Soc Neurosci Abs 2012; 78.04/FF5.Google Scholar

  • [5]

    Bonini L, Ugolotti Serventi F, Bruni S, et al. Selectivity for grip type and action goal in macaque inferior parietal and ventral premotor grasping neurons. J Neurophysiol 2012; 108: 1607–1619.CrossrefWeb of ScienceGoogle Scholar

  • [6]

    Buzsáki G. Large-scale recording of neuronal ensembles. Nat Neurosci 2004; 7: 446–451.CrossrefPubMedGoogle Scholar

  • [7]

    Cho IJ, Baac HW, Yoon E. A 16-site neural probe integrated with a waveguide for optical stimulation. Proc IEEE 23rd Int Micro Electro Mech Syst (MEMS) Conf 2010: 995–998.Google Scholar

  • [8]

    Cohen MR, Kohn A. Measuring and interpreting neuronal correlations. Nat Neurosci 2011; 7: 811–819.Web of ScienceCrossrefGoogle Scholar

  • [9]

    Fekete Z, Hajnal Z, Márton G, Fürjes P, Pongrácz A. Fracture analysis of silicon microprobes designed for deep-brain stimulation. Microelectron Eng 2013; 103: 160–166.Google Scholar

  • [10]

    Gerbella M, Belmalih A, Borra E, Rozzi S, Luppino G. Multimodal architectonic subdivision of the caudal ventrolateral prefrontal cortex of the macaque monkey. Brain Struct Funct 2007; 212: 269–301.Web of ScienceGoogle Scholar

  • [11]

    Grand L, Wittner L, Herwik S, et al. Short and long term biocompatibility of NeuroProbes silicon probes. J Neurosci Methods 2010; 189: 216–229.Web of ScienceGoogle Scholar

  • [12]

    Herwik S, Paul O, Ruther P. Ultrathin silicon chips of arbitrary shape by etching before grinding. J Microelectromech Syst 2011; 20: 791–793.CrossrefWeb of ScienceGoogle Scholar

  • [13]

    Kisban S, Kenntner J, Janssen P, et al. A novel assembly method for silicon-based neural devices. In: World Congress on Medical Physics and Biomedical Engineering, September 7–12, 2009, Munich, Germany, vol. 25/9. Berlin: Springer 2009: 107–110.Google Scholar

  • [14]

    Maranesi M, Livi A, Bonini L, et al. Influence of the sight of monkey’s own acting hand on the motor discharge of mirror neurons. Soc Neurosci Abs 2012; 78.06/FF7.Google Scholar

  • [15]

    Maranesi M, Rodà F, Bonini L, et al. Anatomo-functional organization of the ventral primary motor and premotor cortex in the macaque monkey. Eur J Neurosci 2012; 36: 3376–3387.CrossrefGoogle Scholar

  • [16]

    Márton G, Fekete Z, Fiáth R, et al. In vivo measurements with robust silicon-based multielectrode arrays with extreme shaft lengths. IEEE Sens J 2013; 13: 3263–3269.Web of ScienceCrossrefGoogle Scholar

  • [17]

    Nicolelis MA, Ribeiro S. Multielectrode recordings: the next steps. Curr Opin Neurobiol 2002; 12: 602–606.PubMedCrossrefGoogle Scholar

  • [18]

    Royer S, Zemelman BV, Barbic M, Losonczy A, Buzsáki G, Magee JC. Multi-array silicon probes with integrated optical fibers: light-assisted perturbation and recording of local neural circuits in the behaving animal. Eur J Neurosci 2010; 31: 2279–2291.Web of SciencePubMedCrossrefGoogle Scholar

  • [19]

    Rozzi S, Calzavara R, Belmalih A, et al. Cortical connections of the inferior parietal cortical convexity of the macaque monkey. Cereb Cortex 2006; 16: 1389–1417.Google Scholar

  • [20]

    Sakata S, Harris KD. Laminar structure of spontaneous and sensory-evoked population activity in auditory cortex. Neuron 2009; 64: 404–418.Web of ScienceCrossrefPubMedGoogle Scholar

  • [21]

    Schwaerzle M, Seidl M, Schwarz U, Paul O, Ruther P. Ultracompact optrode with integrated laser diode chips and SU-8: waveguides for optogenetic applications. Tech Dig IEEE MEMS Conf 2013: 1029–1032.Google Scholar

  • [22]

    Super H, Roelfsema P. Chronic multiunit recordings in behaving animals: advantages and limitations. Prog Brain Res 2005; 147: 263–281.Google Scholar

  • [23]

    Ward MP, Rajdev P, Ellison C, Irazoqui PP. Toward a comparison of microelectrodes for acute and chronic recordings. Brain Res 2009; 1282: 183–200.Web of ScienceGoogle Scholar

About the article

Corresponding author: Luca Bonini, PhD, Istituto Italiano di Tecnologia (IIT), Brain Center for Social and Motor Cognition, Via Volturno 39, 43125 Parma, Italy, Phone: +39 521 903847, Fax: +39 521 903900, E-mail:


Received: 2013-04-10

Accepted: 2013-10-01

Published Online: 2013-10-26

Published in Print: 2014-08-01


Citation Information: Biomedical Engineering / Biomedizinische Technik, ISSN (Online) 1862-278X, ISSN (Print) 0013-5585, DOI: https://doi.org/10.1515/bmt-2012-0099.

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[3]
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